lncRNA IGHCγ1 Acts as a ceRNA to Regulate Macrophage Inflammation via the miR-6891-3p/TLR4 Axis in Osteoarthritis
Accumulating data have implicated that long noncoding RNA (lncRNA) plays an important role in osteoarthritis (OA), which may function as a competitive endogenous RNA (ceRNA) of microRNAs (miRNAs). lncRNA IGHCγ1 has been demonstrated to regulate inflammation and autoimmunity. Nonetheless, the alterin...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2020/9743037 |
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| author | Pengjun Zhang Jianmei Sun Caihong Liang Bingjie Gu Yang Xu Hongying Lu Bo Cao Hao Xu |
| author_facet | Pengjun Zhang Jianmei Sun Caihong Liang Bingjie Gu Yang Xu Hongying Lu Bo Cao Hao Xu |
| author_sort | Pengjun Zhang |
| collection | DOAJ |
| description | Accumulating data have implicated that long noncoding RNA (lncRNA) plays an important role in osteoarthritis (OA), which may function as a competitive endogenous RNA (ceRNA) of microRNAs (miRNAs). lncRNA IGHCγ1 has been demonstrated to regulate inflammation and autoimmunity. Nonetheless, the altering effect of IGHCγ1 in OA remains unclear. This study is aimed at investigating the mechanism and function of lncRNA IGHCγ1 in OA. CCK-8, EdU, and transwell assays were used to estimate macrophage proliferation and migration. Fluorescence in situ hybridization (FISH) was performed to estimate the local expression of lncRNA IGHCγ1 in macrophages. Luciferase reporter assay was adopted to validate the ceRNA role of IGHCγ1 as miRNA sponge. lncRNA IGHCγ1 was primarily localized in macrophage cytoplasm and upregulated in OA. miR-6891-3p inhibited macrophage proliferation, migration, and inflammatory response by targeting TLR4, while lncRNA IGHCγ1 promoted TLR4 expression by functioning as a ceRNA for miR-6891-3p through the NF-κB signal in macrophages. This study strongly supports that lncRNA IGHCγ1 regulates inflammatory response via regulating the miR-6891-3p/TLR4/NF-κB axis in macrophages. |
| format | Article |
| id | doaj-art-6352e3191d074b668da14758a49bfdfd |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-6352e3191d074b668da14758a49bfdfd2025-02-03T01:05:06ZengWileyMediators of Inflammation0962-93511466-18612020-01-01202010.1155/2020/97430379743037lncRNA IGHCγ1 Acts as a ceRNA to Regulate Macrophage Inflammation via the miR-6891-3p/TLR4 Axis in OsteoarthritisPengjun Zhang0Jianmei Sun1Caihong Liang2Bingjie Gu3Yang Xu4Hongying Lu5Bo Cao6Hao Xu7Department of Joint Surgery, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao 266100, ChinaDepartment of Chemistry, School of Applied Chemistry, Food and Drug, Weifang Engineering Vocational College, Qingzhou 262500, ChinaDepartment of Cardiovasology, The Affiliated Jiangning Hospital of Nanjing Medical University, Nanjing 210000, ChinaDepartment of Rheumatology and Immunology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, ChinaNational Center for Occupational Safety and Health, NHC, Beijing 100023, ChinaFunctional Laboratory, Weifang Medical University, Weifang 261000, ChinaDepartment of Emergency Medicine, Affiliated Hospital of Weifang Medical University, Weifang 261000, ChinaDepartment of Joint Surgery, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao 266100, ChinaAccumulating data have implicated that long noncoding RNA (lncRNA) plays an important role in osteoarthritis (OA), which may function as a competitive endogenous RNA (ceRNA) of microRNAs (miRNAs). lncRNA IGHCγ1 has been demonstrated to regulate inflammation and autoimmunity. Nonetheless, the altering effect of IGHCγ1 in OA remains unclear. This study is aimed at investigating the mechanism and function of lncRNA IGHCγ1 in OA. CCK-8, EdU, and transwell assays were used to estimate macrophage proliferation and migration. Fluorescence in situ hybridization (FISH) was performed to estimate the local expression of lncRNA IGHCγ1 in macrophages. Luciferase reporter assay was adopted to validate the ceRNA role of IGHCγ1 as miRNA sponge. lncRNA IGHCγ1 was primarily localized in macrophage cytoplasm and upregulated in OA. miR-6891-3p inhibited macrophage proliferation, migration, and inflammatory response by targeting TLR4, while lncRNA IGHCγ1 promoted TLR4 expression by functioning as a ceRNA for miR-6891-3p through the NF-κB signal in macrophages. This study strongly supports that lncRNA IGHCγ1 regulates inflammatory response via regulating the miR-6891-3p/TLR4/NF-κB axis in macrophages.http://dx.doi.org/10.1155/2020/9743037 |
| spellingShingle | Pengjun Zhang Jianmei Sun Caihong Liang Bingjie Gu Yang Xu Hongying Lu Bo Cao Hao Xu lncRNA IGHCγ1 Acts as a ceRNA to Regulate Macrophage Inflammation via the miR-6891-3p/TLR4 Axis in Osteoarthritis Mediators of Inflammation |
| title | lncRNA IGHCγ1 Acts as a ceRNA to Regulate Macrophage Inflammation via the miR-6891-3p/TLR4 Axis in Osteoarthritis |
| title_full | lncRNA IGHCγ1 Acts as a ceRNA to Regulate Macrophage Inflammation via the miR-6891-3p/TLR4 Axis in Osteoarthritis |
| title_fullStr | lncRNA IGHCγ1 Acts as a ceRNA to Regulate Macrophage Inflammation via the miR-6891-3p/TLR4 Axis in Osteoarthritis |
| title_full_unstemmed | lncRNA IGHCγ1 Acts as a ceRNA to Regulate Macrophage Inflammation via the miR-6891-3p/TLR4 Axis in Osteoarthritis |
| title_short | lncRNA IGHCγ1 Acts as a ceRNA to Regulate Macrophage Inflammation via the miR-6891-3p/TLR4 Axis in Osteoarthritis |
| title_sort | lncrna ighcγ1 acts as a cerna to regulate macrophage inflammation via the mir 6891 3p tlr4 axis in osteoarthritis |
| url | http://dx.doi.org/10.1155/2020/9743037 |
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