Spatiotemporal Expression and Molecular Characterization of miR-344b and miR-344c in the Developing Mouse Brain
MicroRNAs (miRNAs) are small noncoding RNA known to regulate brain development. The expression of two novel miRNAs, namely, miR-344b and miR-344c, was characterized during mouse brain developmental stages in this study. In situ hybridization analysis showed that miR-344b and miR-344c were expressed...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2016/1951250 |
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| author | Jia-Wen Leong Syahril Abdullah King-Hwa Ling Pike-See Cheah |
| author_facet | Jia-Wen Leong Syahril Abdullah King-Hwa Ling Pike-See Cheah |
| author_sort | Jia-Wen Leong |
| collection | DOAJ |
| description | MicroRNAs (miRNAs) are small noncoding RNA known to regulate brain development. The expression of two novel miRNAs, namely, miR-344b and miR-344c, was characterized during mouse brain developmental stages in this study. In situ hybridization analysis showed that miR-344b and miR-344c were expressed in the germinal layer during embryonic brain developmental stages. In contrast, miR-344b was not detectable in the adult brain while miR-344c was expressed exclusively in the adult olfactory bulb and cerebellar granular layer. Stem-loop RT-qPCR analysis of whole brain RNAs showed that expression of the miR-344b and miR-344c was increased as brain developed throughout the embryonic stage and maintained at adulthood. Further investigation showed that these miRNAs were expressed in adult organs, where miR-344b and miR-344c were highly expressed in pancreas and brain, respectively. Bioinformatics analysis suggested miR-344b and miR-344c targeted Olig2 and Otx2 mRNAs, respectively. However, luciferase experiments demonstrated that these miRNAs did not target Olig2 and Otx2 mRNAs. Further investigation on the locality of miR-344b and miR-344c showed that both miRNAs were localized in nuclei of immature neurons. In conclusion, miR-344b and miR-344c were expressed spatiotemporally during mouse brain developmental stages. |
| format | Article |
| id | doaj-art-632d5d59d5394c97abe04497eef46711 |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-632d5d59d5394c97abe04497eef467112025-02-03T05:58:40ZengWileyNeural Plasticity2090-59041687-54432016-01-01201610.1155/2016/19512501951250Spatiotemporal Expression and Molecular Characterization of miR-344b and miR-344c in the Developing Mouse BrainJia-Wen Leong0Syahril Abdullah1King-Hwa Ling2Pike-See Cheah3Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, MalaysiaGenetics and Regenerative Medicine Research Centre (GRMRC), Faculty of Medicine and Health Sciences, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, MalaysiaGenetics and Regenerative Medicine Research Centre (GRMRC), Faculty of Medicine and Health Sciences, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, MalaysiaDepartment of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, MalaysiaMicroRNAs (miRNAs) are small noncoding RNA known to regulate brain development. The expression of two novel miRNAs, namely, miR-344b and miR-344c, was characterized during mouse brain developmental stages in this study. In situ hybridization analysis showed that miR-344b and miR-344c were expressed in the germinal layer during embryonic brain developmental stages. In contrast, miR-344b was not detectable in the adult brain while miR-344c was expressed exclusively in the adult olfactory bulb and cerebellar granular layer. Stem-loop RT-qPCR analysis of whole brain RNAs showed that expression of the miR-344b and miR-344c was increased as brain developed throughout the embryonic stage and maintained at adulthood. Further investigation showed that these miRNAs were expressed in adult organs, where miR-344b and miR-344c were highly expressed in pancreas and brain, respectively. Bioinformatics analysis suggested miR-344b and miR-344c targeted Olig2 and Otx2 mRNAs, respectively. However, luciferase experiments demonstrated that these miRNAs did not target Olig2 and Otx2 mRNAs. Further investigation on the locality of miR-344b and miR-344c showed that both miRNAs were localized in nuclei of immature neurons. In conclusion, miR-344b and miR-344c were expressed spatiotemporally during mouse brain developmental stages.http://dx.doi.org/10.1155/2016/1951250 |
| spellingShingle | Jia-Wen Leong Syahril Abdullah King-Hwa Ling Pike-See Cheah Spatiotemporal Expression and Molecular Characterization of miR-344b and miR-344c in the Developing Mouse Brain Neural Plasticity |
| title | Spatiotemporal Expression and Molecular Characterization of miR-344b and miR-344c in the Developing Mouse Brain |
| title_full | Spatiotemporal Expression and Molecular Characterization of miR-344b and miR-344c in the Developing Mouse Brain |
| title_fullStr | Spatiotemporal Expression and Molecular Characterization of miR-344b and miR-344c in the Developing Mouse Brain |
| title_full_unstemmed | Spatiotemporal Expression and Molecular Characterization of miR-344b and miR-344c in the Developing Mouse Brain |
| title_short | Spatiotemporal Expression and Molecular Characterization of miR-344b and miR-344c in the Developing Mouse Brain |
| title_sort | spatiotemporal expression and molecular characterization of mir 344b and mir 344c in the developing mouse brain |
| url | http://dx.doi.org/10.1155/2016/1951250 |
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