Alleviating effects and mechanisms of action of large-leaf yellow tea drinking on diabetes and diabetic nephropathy in mice
Our previous study found that large-leaf yellow tea (LYT) had interesting hypoglycemic activity in high-fat diet-induced obese mice and highly safety in healthy mice. To study the anti-diabetic potential of LYT, the present study further investigated the preventive effects and mechanisms of action o...
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Tsinghua University Press
2023-09-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S221345302300023X |
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| author | Guangshan Zhao Jianyuan Teng Ruixia Dong Qiuyan Ban Lian Yang Kang Du Yifei Wang Hanlin Pu Chung S. Yang Zhe Ren |
| author_facet | Guangshan Zhao Jianyuan Teng Ruixia Dong Qiuyan Ban Lian Yang Kang Du Yifei Wang Hanlin Pu Chung S. Yang Zhe Ren |
| author_sort | Guangshan Zhao |
| collection | DOAJ |
| description | Our previous study found that large-leaf yellow tea (LYT) had interesting hypoglycemic activity in high-fat diet-induced obese mice and highly safety in healthy mice. To study the anti-diabetic potential of LYT, the present study further investigated the preventive effects and mechanisms of action of LYT administration on diabetes and diabetic nephropathy in high-fat diet plus streptozotocin-induced diabetic mice. Results showed that LYT infusions (1/100 and 1/50, m/V) as drinking fluid for 4 weeks reduced diabetic polydipsia and polyuria, enhanced glucose tolerance and insulin sensitivity, and lowered fasting blood glucose level. The underlying mechanisms involve downregulation of gluconeogenesis (lower protein levels of TXNIP and FBP and enzyme activity of FBP), upregulation of lipid catabolism (higher protein levels of CPT-1α and PPARα), downregulation of lipogenesis (lower protein level of SREBP-1), and modification of the structure and abundance of gut microbiota to modulate metabolic homeostasis. Moreover, LYT administration prevented diabetic nephropathy, possibly due to reduced glucose-caused osmotic diuresis and lowered levels of renal PKC-β2, NLRP3 as well as membrane PKC-α, AQP2 and glycosylated AQP2 proteins. Taken together, LYT exhibits the activities in alleviating diabetic symptoms, ameliorating glucose and lipid dysmetabolism and fatty liver, and preventing diabetic nephropathy in diabetic mice. These activities may be explored for the prevention and treatment of diabetes in humans. |
| format | Article |
| id | doaj-art-630e3b815cac4762b04eb98acc597fd4 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-630e3b815cac4762b04eb98acc597fd42025-02-03T00:27:37ZengTsinghua University PressFood Science and Human Wellness2213-45302023-09-0112516601673Alleviating effects and mechanisms of action of large-leaf yellow tea drinking on diabetes and diabetic nephropathy in miceGuangshan Zhao0Jianyuan Teng1Ruixia Dong2Qiuyan Ban3Lian Yang4Kang Du5Yifei Wang6Hanlin Pu7Chung S. Yang8Zhe Ren9Innovation Team of Food Nutrition and Safety Control, College of Food Science & Technology, Henan Agricultural University, Zhengzhou 450002, China; Biology Postdoctoral Research Station, Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou 510632, ChinaBiology Postdoctoral Research Station, Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou 510632, ChinaCollege of Horticulture, Jinling Institute of Technology, Nanjing 211169, China; Department of Forestry and Technology, Lishui Vocational and Technical College, Lishui 323000, ChinaDepartment of Tea Science, College of Horticulture, Henan Agricultural University, Zhengzhou 450002, China; Corresponding authors.Guangdong Provincial Engineering Center of Topical Precise Drug Delivery System, School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaGuangdong Provincial Engineering Center of Topical Precise Drug Delivery System, School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaBiology Postdoctoral Research Station, Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou 510632, ChinaBiology Postdoctoral Research Station, Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China; Corresponding authors.Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway 08901, USA; International Joint Research Laboratory of Tea Chemistry and Health Effects, Anhui Agricultural University, Hefei 230036, China; Corresponding authors.Biology Postdoctoral Research Station, Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China; Corresponding authors.Our previous study found that large-leaf yellow tea (LYT) had interesting hypoglycemic activity in high-fat diet-induced obese mice and highly safety in healthy mice. To study the anti-diabetic potential of LYT, the present study further investigated the preventive effects and mechanisms of action of LYT administration on diabetes and diabetic nephropathy in high-fat diet plus streptozotocin-induced diabetic mice. Results showed that LYT infusions (1/100 and 1/50, m/V) as drinking fluid for 4 weeks reduced diabetic polydipsia and polyuria, enhanced glucose tolerance and insulin sensitivity, and lowered fasting blood glucose level. The underlying mechanisms involve downregulation of gluconeogenesis (lower protein levels of TXNIP and FBP and enzyme activity of FBP), upregulation of lipid catabolism (higher protein levels of CPT-1α and PPARα), downregulation of lipogenesis (lower protein level of SREBP-1), and modification of the structure and abundance of gut microbiota to modulate metabolic homeostasis. Moreover, LYT administration prevented diabetic nephropathy, possibly due to reduced glucose-caused osmotic diuresis and lowered levels of renal PKC-β2, NLRP3 as well as membrane PKC-α, AQP2 and glycosylated AQP2 proteins. Taken together, LYT exhibits the activities in alleviating diabetic symptoms, ameliorating glucose and lipid dysmetabolism and fatty liver, and preventing diabetic nephropathy in diabetic mice. These activities may be explored for the prevention and treatment of diabetes in humans.http://www.sciencedirect.com/science/article/pii/S221345302300023XDiabetesGlucose and lipid metabolismGut microbiotaDiabetic nephropathyLarge-leaf yellow tea |
| spellingShingle | Guangshan Zhao Jianyuan Teng Ruixia Dong Qiuyan Ban Lian Yang Kang Du Yifei Wang Hanlin Pu Chung S. Yang Zhe Ren Alleviating effects and mechanisms of action of large-leaf yellow tea drinking on diabetes and diabetic nephropathy in mice Food Science and Human Wellness Diabetes Glucose and lipid metabolism Gut microbiota Diabetic nephropathy Large-leaf yellow tea |
| title | Alleviating effects and mechanisms of action of large-leaf yellow tea drinking on diabetes and diabetic nephropathy in mice |
| title_full | Alleviating effects and mechanisms of action of large-leaf yellow tea drinking on diabetes and diabetic nephropathy in mice |
| title_fullStr | Alleviating effects and mechanisms of action of large-leaf yellow tea drinking on diabetes and diabetic nephropathy in mice |
| title_full_unstemmed | Alleviating effects and mechanisms of action of large-leaf yellow tea drinking on diabetes and diabetic nephropathy in mice |
| title_short | Alleviating effects and mechanisms of action of large-leaf yellow tea drinking on diabetes and diabetic nephropathy in mice |
| title_sort | alleviating effects and mechanisms of action of large leaf yellow tea drinking on diabetes and diabetic nephropathy in mice |
| topic | Diabetes Glucose and lipid metabolism Gut microbiota Diabetic nephropathy Large-leaf yellow tea |
| url | http://www.sciencedirect.com/science/article/pii/S221345302300023X |
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