Purification and Characterization of a Low Molecular Weight Neutral Non-Starch Polysaccharide from Panax ginsen g by Enzymatic Hydrolysis
In this study, a novel neutral non-starch polysaccharide (GP1A) was extracted using hot water from ginseng roots. Chemical characteristics, monosaccharide compositions and structure of GP1A were investigated using scanning electron microscopy, gel permeation chromatography, X-ray diffraction, Fourie...
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Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences
2023-02-01
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| Series: | Polish Journal of Food and Nutrition Sciences |
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| Online Access: | http://journal.pan.olsztyn.pl/Purification-and-Characterization-of-a-Low-Molecular-Weight-Neutral-Non-Starch-Polysaccharide,160179,0,2.html |
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| author | Ying Ying Chao Ma Yajie Zhang Xiaoping Li Hongxin Wu |
| author_facet | Ying Ying Chao Ma Yajie Zhang Xiaoping Li Hongxin Wu |
| author_sort | Ying Ying |
| collection | DOAJ |
| description | In this study, a novel neutral non-starch polysaccharide (GP1A) was extracted using hot water from ginseng roots. Chemical characteristics, monosaccharide compositions and structure of GP1A were investigated using scanning electron microscopy, gel permeation chromatography, X-ray diffraction, Fourier transform infrared spectroscopy, gas chromatography with mass spectrometer, one-dimensional and two-dimensional nuclear magnetic resonance. The results indicated that the lyophilized GP1A was a homogeneous polysaccharide with a low molecular weight of 1.03 kDa. The dominating monosaccharides of GP1A were D-glucose, D-galactose, D-mannose, D-xylose and D-arabinose. The structure analysis indicated the main chain residues sequence of GP1A was α-D-Glc p -(6→3)-α-D-Gal p -(1→1)-α-D-Glc p -(3→4)-β-D-Man p -(2→2)-β-D-Ara p with branch chains of α-D-Glc p substituted at α-D-Gal p and β-D-Xyl p substituted at β-D-Manp. |
| format | Article |
| id | doaj-art-62dce85b87f040f9abfa004afcf710b7 |
| institution | Kabale University |
| issn | 2083-6007 |
| language | English |
| publishDate | 2023-02-01 |
| publisher | Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences |
| record_format | Article |
| series | Polish Journal of Food and Nutrition Sciences |
| spelling | doaj-art-62dce85b87f040f9abfa004afcf710b72025-02-02T23:55:06ZengInstitute of Animal Reproduction and Food Research of the Polish Academy of SciencesPolish Journal of Food and Nutrition Sciences2083-60072023-02-01731707910.31883/pjfns/160179160179Purification and Characterization of a Low Molecular Weight Neutral Non-Starch Polysaccharide from Panax ginsen g by Enzymatic HydrolysisYing Ying0https://orcid.org/0000-0002-2926-6051Chao Ma1https://orcid.org/0000-0001-6728-4114Yajie Zhang2https://orcid.org/0000-0003-2278-5905Xiaoping Li3https://orcid.org/0000-0001-5451-9404Hongxin Wu4https://orcid.org/0000-0003-4809-5419Institute of Grassland Research, Chinese Academy of Agricultural Science, Huhhot, Inner Mongolia, 010010, P.R. ChinaBeijing Key Laboratory of Forest Food Processing and Safety, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, P.R. ChinaBeijing Key Laboratory of Forest Food Processing and Safety, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, P.R. ChinaBeijing Key Laboratory of Forest Food Processing and Safety, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, P.R. ChinaInstitute of Grassland Research, Chinese Academy of Agricultural Science, Huhhot, Inner Mongolia, 010010, P.R. ChinaIn this study, a novel neutral non-starch polysaccharide (GP1A) was extracted using hot water from ginseng roots. Chemical characteristics, monosaccharide compositions and structure of GP1A were investigated using scanning electron microscopy, gel permeation chromatography, X-ray diffraction, Fourier transform infrared spectroscopy, gas chromatography with mass spectrometer, one-dimensional and two-dimensional nuclear magnetic resonance. The results indicated that the lyophilized GP1A was a homogeneous polysaccharide with a low molecular weight of 1.03 kDa. The dominating monosaccharides of GP1A were D-glucose, D-galactose, D-mannose, D-xylose and D-arabinose. The structure analysis indicated the main chain residues sequence of GP1A was α-D-Glc p -(6→3)-α-D-Gal p -(1→1)-α-D-Glc p -(3→4)-β-D-Man p -(2→2)-β-D-Ara p with branch chains of α-D-Glc p substituted at α-D-Gal p and β-D-Xyl p substituted at β-D-Manp.http://journal.pan.olsztyn.pl/Purification-and-Characterization-of-a-Low-Molecular-Weight-Neutral-Non-Starch-Polysaccharide,160179,0,2.htmlginseng polysaccharidechromatographic separationmonosaccharide compositionstructure analysis |
| spellingShingle | Ying Ying Chao Ma Yajie Zhang Xiaoping Li Hongxin Wu Purification and Characterization of a Low Molecular Weight Neutral Non-Starch Polysaccharide from Panax ginsen g by Enzymatic Hydrolysis Polish Journal of Food and Nutrition Sciences ginseng polysaccharide chromatographic separation monosaccharide composition structure analysis |
| title | Purification and Characterization of a Low Molecular Weight Neutral Non-Starch Polysaccharide from Panax ginsen g by Enzymatic Hydrolysis |
| title_full | Purification and Characterization of a Low Molecular Weight Neutral Non-Starch Polysaccharide from Panax ginsen g by Enzymatic Hydrolysis |
| title_fullStr | Purification and Characterization of a Low Molecular Weight Neutral Non-Starch Polysaccharide from Panax ginsen g by Enzymatic Hydrolysis |
| title_full_unstemmed | Purification and Characterization of a Low Molecular Weight Neutral Non-Starch Polysaccharide from Panax ginsen g by Enzymatic Hydrolysis |
| title_short | Purification and Characterization of a Low Molecular Weight Neutral Non-Starch Polysaccharide from Panax ginsen g by Enzymatic Hydrolysis |
| title_sort | purification and characterization of a low molecular weight neutral non starch polysaccharide from panax ginsen g by enzymatic hydrolysis |
| topic | ginseng polysaccharide chromatographic separation monosaccharide composition structure analysis |
| url | http://journal.pan.olsztyn.pl/Purification-and-Characterization-of-a-Low-Molecular-Weight-Neutral-Non-Starch-Polysaccharide,160179,0,2.html |
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