Coagulase gene polymorphism of Staphylococcus aureus isolates: A study on dairy food products and other foods in Tehran, Iran
Coagulase is considered as a major determinant factor for the identification of Staphylococcus aureus strains. The 3′-end coding region of the coagulase (coa) gene contains a series of 81-bp tandem repeats, which differ in the number and location of enzymatic restriction sites among different isolat...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2016-12-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453016300349 |
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| author | Mohammad Mehdi Soltan Dallal Mohammad Reza Khoramizadeh Solmaz Agha Amiri Ali Akbar Saboor Yaraghi Ramin Mazaheri Nezhad Fard |
| author_facet | Mohammad Mehdi Soltan Dallal Mohammad Reza Khoramizadeh Solmaz Agha Amiri Ali Akbar Saboor Yaraghi Ramin Mazaheri Nezhad Fard |
| author_sort | Mohammad Mehdi Soltan Dallal |
| collection | DOAJ |
| description | Coagulase is considered as a major determinant factor for the identification of Staphylococcus aureus strains. The 3′-end coding region of the coagulase (coa) gene contains a series of 81-bp tandem repeats, which differ in the number and location of enzymatic restriction sites among different isolates. coa PCR-RFLP has been used widely to type S. aureus isolates in epidemiological studies. The current study was conducted to investigate the coagulase gene polymorphisms in S. aureus isolated from various food samples using an in house PCR-RFLP method. A total of 100 strains of S. aureus were isolated from food samples. Isolates were typed by PCR-RFLP analysis using NdeI restriction digestion of the coagulase gene PCR products. Results showed that amplification of coagulase genes from S. aureus produced different PCR products. The isolates were grouped into 18 genotypes using RFLP analysis results of the genes. In this study, the S. aureus isolates have been shown to include more than one coagulase genotype, but only had a few coa genotypes predominated. |
| format | Article |
| id | doaj-art-62da6b3569d04249988f49c4e97c5f5e |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2016-12-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-62da6b3569d04249988f49c4e97c5f5e2025-02-03T11:07:58ZengTsinghua University PressFood Science and Human Wellness2213-45302016-12-015418619010.1016/j.fshw.2016.09.004Coagulase gene polymorphism of Staphylococcus aureus isolates: A study on dairy food products and other foods in Tehran, IranMohammad Mehdi Soltan Dallal0Mohammad Reza Khoramizadeh1Solmaz Agha Amiri2Ali Akbar Saboor Yaraghi3Ramin Mazaheri Nezhad Fard4Division of Food Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Medical Biotechnology, School of Advanced Technologies in Medicine, IranFood Microbiology Research Center, Tehran University of Medical Sciences, Tehran, IranFood Microbiology Research Center, Tehran University of Medical Sciences, Tehran, IranDivision of Food Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranCoagulase is considered as a major determinant factor for the identification of Staphylococcus aureus strains. The 3′-end coding region of the coagulase (coa) gene contains a series of 81-bp tandem repeats, which differ in the number and location of enzymatic restriction sites among different isolates. coa PCR-RFLP has been used widely to type S. aureus isolates in epidemiological studies. The current study was conducted to investigate the coagulase gene polymorphisms in S. aureus isolated from various food samples using an in house PCR-RFLP method. A total of 100 strains of S. aureus were isolated from food samples. Isolates were typed by PCR-RFLP analysis using NdeI restriction digestion of the coagulase gene PCR products. Results showed that amplification of coagulase genes from S. aureus produced different PCR products. The isolates were grouped into 18 genotypes using RFLP analysis results of the genes. In this study, the S. aureus isolates have been shown to include more than one coagulase genotype, but only had a few coa genotypes predominated.http://www.sciencedirect.com/science/article/pii/S2213453016300349Staphylococcus aureusPCR-RFLPCoagulase geneFood samples |
| spellingShingle | Mohammad Mehdi Soltan Dallal Mohammad Reza Khoramizadeh Solmaz Agha Amiri Ali Akbar Saboor Yaraghi Ramin Mazaheri Nezhad Fard Coagulase gene polymorphism of Staphylococcus aureus isolates: A study on dairy food products and other foods in Tehran, Iran Food Science and Human Wellness Staphylococcus aureus PCR-RFLP Coagulase gene Food samples |
| title | Coagulase gene polymorphism of Staphylococcus aureus isolates: A study on dairy food products and other foods in Tehran, Iran |
| title_full | Coagulase gene polymorphism of Staphylococcus aureus isolates: A study on dairy food products and other foods in Tehran, Iran |
| title_fullStr | Coagulase gene polymorphism of Staphylococcus aureus isolates: A study on dairy food products and other foods in Tehran, Iran |
| title_full_unstemmed | Coagulase gene polymorphism of Staphylococcus aureus isolates: A study on dairy food products and other foods in Tehran, Iran |
| title_short | Coagulase gene polymorphism of Staphylococcus aureus isolates: A study on dairy food products and other foods in Tehran, Iran |
| title_sort | coagulase gene polymorphism of staphylococcus aureus isolates a study on dairy food products and other foods in tehran iran |
| topic | Staphylococcus aureus PCR-RFLP Coagulase gene Food samples |
| url | http://www.sciencedirect.com/science/article/pii/S2213453016300349 |
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