S100A8/A9 Molecular Complexes Promote Cancer Migration and Invasion via the p38 MAPK Pathway in Nasopharyngeal Carcinoma
Nasopharyngeal carcinoma (NPC) is one type of malignancy associated with migration and invasion through a currently unclear mechanism. We previously discovered S100A8/A9 levels were roughly elevated in the plasma of NPC patients as the promising biomarkers. However, their expressions and underlying...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Bioinorganic Chemistry and Applications |
| Online Access: | http://dx.doi.org/10.1155/2021/9913794 |
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| author | Ning Xu Bei-Bei Zhang Xia-Ning Huang Xiang Yi Xue-Min Yan Yan Cai Qin He Zi-Jian Han Yuan-Jiao Huang Wei Liu Ai-Jun Jiao |
| author_facet | Ning Xu Bei-Bei Zhang Xia-Ning Huang Xiang Yi Xue-Min Yan Yan Cai Qin He Zi-Jian Han Yuan-Jiao Huang Wei Liu Ai-Jun Jiao |
| author_sort | Ning Xu |
| collection | DOAJ |
| description | Nasopharyngeal carcinoma (NPC) is one type of malignancy associated with migration and invasion through a currently unclear mechanism. We previously discovered S100A8/A9 levels were roughly elevated in the plasma of NPC patients as the promising biomarkers. However, their expressions and underlying functions in NPC tissues are still unknown. In the present study, we analyzed 49 NPC tissues and 20 chronic pharyngitis (CP) tissues. Immunohistochemical staining was performed in different tissues and analyzed by the Mann–Whitney U test statistically. Transwell migration and invasion experiments were further performed to determine S100A8/A9 effects on NPC. Our results showed that S100A8/A9 in NPC tissues were significantly higher than those in CP tissues, closely associated with NPC clinical stages. Intriguingly, exogenous S100A8/A9 protein stimulation could dramatically enhance NPC migration and invasion abilities. In addition, p38 MAPK pathway blockade could diminish the migration and invasion of NPC cells stimulated by S100A8/A9 proteins. The downstream tumor invasion and migration associated proteins (e.g., MMP7) were also elevated in NPC tissues, consistent with S100A8/A9 overexpression. Taken together, our present findings suggest that the secreted soluble inflammatory factors S100A8/A9 might promote cancer migration and invasion via the p38 MAPK signaling pathway along with invasion/migration associated proteins overexpression in the tumor microenvironment of NPC. This may shed light on the mechanism understanding of NPC prognosis and provide more novel clues for NPC diagnosis and therapy. |
| format | Article |
| id | doaj-art-62b1a8c1977241ac81657e8cb3f8efba |
| institution | Kabale University |
| issn | 1565-3633 1687-479X |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioinorganic Chemistry and Applications |
| spelling | doaj-art-62b1a8c1977241ac81657e8cb3f8efba2025-02-03T07:24:02ZengWileyBioinorganic Chemistry and Applications1565-36331687-479X2021-01-01202110.1155/2021/99137949913794S100A8/A9 Molecular Complexes Promote Cancer Migration and Invasion via the p38 MAPK Pathway in Nasopharyngeal CarcinomaNing Xu0Bei-Bei Zhang1Xia-Ning Huang2Xiang Yi3Xue-Min Yan4Yan Cai5Qin He6Zi-Jian Han7Yuan-Jiao Huang8Wei Liu9Ai-Jun Jiao10The Fifth Affiliated Hospital of Guangxi Medical University, Nanning, ChinaInstitute of Biomedical Research, Yunnan University, Kunming, ChinaWuming Hospital of Guangxi Mediacal University, Nanning, ChinaDepartment of Otolaryngology-Head and Neck Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, ChinaGraduate School of Guangxi Medical University, Nanning, ChinaGraduate School of Guangxi Medical University, Nanning, ChinaGraduate School of Guangxi Medical University, Nanning, ChinaGraduate School of Guangxi Medical University, Nanning, ChinaLife Science Institute, Guangxi Medical University, Nanning, ChinaPharmaceutical College, Guangxi Medical University, Nanning, ChinaPharmaceutical College, Guangxi Medical University, Nanning, ChinaNasopharyngeal carcinoma (NPC) is one type of malignancy associated with migration and invasion through a currently unclear mechanism. We previously discovered S100A8/A9 levels were roughly elevated in the plasma of NPC patients as the promising biomarkers. However, their expressions and underlying functions in NPC tissues are still unknown. In the present study, we analyzed 49 NPC tissues and 20 chronic pharyngitis (CP) tissues. Immunohistochemical staining was performed in different tissues and analyzed by the Mann–Whitney U test statistically. Transwell migration and invasion experiments were further performed to determine S100A8/A9 effects on NPC. Our results showed that S100A8/A9 in NPC tissues were significantly higher than those in CP tissues, closely associated with NPC clinical stages. Intriguingly, exogenous S100A8/A9 protein stimulation could dramatically enhance NPC migration and invasion abilities. In addition, p38 MAPK pathway blockade could diminish the migration and invasion of NPC cells stimulated by S100A8/A9 proteins. The downstream tumor invasion and migration associated proteins (e.g., MMP7) were also elevated in NPC tissues, consistent with S100A8/A9 overexpression. Taken together, our present findings suggest that the secreted soluble inflammatory factors S100A8/A9 might promote cancer migration and invasion via the p38 MAPK signaling pathway along with invasion/migration associated proteins overexpression in the tumor microenvironment of NPC. This may shed light on the mechanism understanding of NPC prognosis and provide more novel clues for NPC diagnosis and therapy.http://dx.doi.org/10.1155/2021/9913794 |
| spellingShingle | Ning Xu Bei-Bei Zhang Xia-Ning Huang Xiang Yi Xue-Min Yan Yan Cai Qin He Zi-Jian Han Yuan-Jiao Huang Wei Liu Ai-Jun Jiao S100A8/A9 Molecular Complexes Promote Cancer Migration and Invasion via the p38 MAPK Pathway in Nasopharyngeal Carcinoma Bioinorganic Chemistry and Applications |
| title | S100A8/A9 Molecular Complexes Promote Cancer Migration and Invasion via the p38 MAPK Pathway in Nasopharyngeal Carcinoma |
| title_full | S100A8/A9 Molecular Complexes Promote Cancer Migration and Invasion via the p38 MAPK Pathway in Nasopharyngeal Carcinoma |
| title_fullStr | S100A8/A9 Molecular Complexes Promote Cancer Migration and Invasion via the p38 MAPK Pathway in Nasopharyngeal Carcinoma |
| title_full_unstemmed | S100A8/A9 Molecular Complexes Promote Cancer Migration and Invasion via the p38 MAPK Pathway in Nasopharyngeal Carcinoma |
| title_short | S100A8/A9 Molecular Complexes Promote Cancer Migration and Invasion via the p38 MAPK Pathway in Nasopharyngeal Carcinoma |
| title_sort | s100a8 a9 molecular complexes promote cancer migration and invasion via the p38 mapk pathway in nasopharyngeal carcinoma |
| url | http://dx.doi.org/10.1155/2021/9913794 |
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