Evaluation of the Cytotoxicity of Satureja spicigera and Its Main Compounds
Satureja spicigera (Lamiaceae) grows wildly in Northwest of Iran. In this study, bioassay-guided isolation and identification of the main compounds has been reported using various chromatographic methods and comparison of their spectral data with those reported in the literature. Brine shrimp lethal...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/203861 |
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| author | Ahmad Reza Gohari Seyed Nasser Ostad Fahimeh Moradi-Afrapoli Maryam Malmir Shohreh Tavajohi Hassan Akbari Soodabeh Saeidnia |
| author_facet | Ahmad Reza Gohari Seyed Nasser Ostad Fahimeh Moradi-Afrapoli Maryam Malmir Shohreh Tavajohi Hassan Akbari Soodabeh Saeidnia |
| author_sort | Ahmad Reza Gohari |
| collection | DOAJ |
| description | Satureja spicigera (Lamiaceae) grows wildly in Northwest of Iran. In this study, bioassay-guided isolation and identification of the main compounds has been reported using various chromatographic methods and comparison of their spectral data with those reported in the literature. Brine shrimp lethality and four cancerous cell lines HT29/219, Caco2, NIH-3T3, and T47D were used for cytotoxicity evaluations. From the aerial parts of S. spicigera, nine known compounds including two flavanones, 5,7,3,5-tetrahydroxy flavanone (8) and 5,4-dihydroxy-3-methoxyflavanone-7-(6-O-α-L-rhamnopyranosyl)-β-D-glucopyranoside (9), one dihydrochalcone, nubigenol (7), together with thymoquinone (1), thymol (2), carvacrol (3), β-sitosterol (4), ursolic acid (5) and oleanolic acid (6) were identified. Among the isolated chalcone and flavanones, compound 8 was effective against Artemia salina larva (LC50= 2 μg/mL) and only the compound 9 demonstrated IC50 value of 98.7 μg/mL on the T47D (human, breast, ductal carcinoma). Other compounds did not show significant inhibition of the cell growth. |
| format | Article |
| id | doaj-art-62af152bab30406b80d2147ee5aecd2c |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-62af152bab30406b80d2147ee5aecd2c2025-02-03T06:00:20ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/203861203861Evaluation of the Cytotoxicity of Satureja spicigera and Its Main CompoundsAhmad Reza Gohari0Seyed Nasser Ostad1Fahimeh Moradi-Afrapoli2Maryam Malmir3Shohreh Tavajohi4Hassan Akbari5Soodabeh Saeidnia6Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran 14176 14411, IranDepartment of Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 14176 14411, IranMedicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran 14176 14411, IranMedicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran 14176 14411, IranDepartment of Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 14176 14411, IranDepartment of Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 14176 14411, IranMedicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran 14176 14411, IranSatureja spicigera (Lamiaceae) grows wildly in Northwest of Iran. In this study, bioassay-guided isolation and identification of the main compounds has been reported using various chromatographic methods and comparison of their spectral data with those reported in the literature. Brine shrimp lethality and four cancerous cell lines HT29/219, Caco2, NIH-3T3, and T47D were used for cytotoxicity evaluations. From the aerial parts of S. spicigera, nine known compounds including two flavanones, 5,7,3,5-tetrahydroxy flavanone (8) and 5,4-dihydroxy-3-methoxyflavanone-7-(6-O-α-L-rhamnopyranosyl)-β-D-glucopyranoside (9), one dihydrochalcone, nubigenol (7), together with thymoquinone (1), thymol (2), carvacrol (3), β-sitosterol (4), ursolic acid (5) and oleanolic acid (6) were identified. Among the isolated chalcone and flavanones, compound 8 was effective against Artemia salina larva (LC50= 2 μg/mL) and only the compound 9 demonstrated IC50 value of 98.7 μg/mL on the T47D (human, breast, ductal carcinoma). Other compounds did not show significant inhibition of the cell growth.http://dx.doi.org/10.1100/2012/203861 |
| spellingShingle | Ahmad Reza Gohari Seyed Nasser Ostad Fahimeh Moradi-Afrapoli Maryam Malmir Shohreh Tavajohi Hassan Akbari Soodabeh Saeidnia Evaluation of the Cytotoxicity of Satureja spicigera and Its Main Compounds The Scientific World Journal |
| title | Evaluation of the Cytotoxicity of Satureja spicigera and Its Main Compounds |
| title_full | Evaluation of the Cytotoxicity of Satureja spicigera and Its Main Compounds |
| title_fullStr | Evaluation of the Cytotoxicity of Satureja spicigera and Its Main Compounds |
| title_full_unstemmed | Evaluation of the Cytotoxicity of Satureja spicigera and Its Main Compounds |
| title_short | Evaluation of the Cytotoxicity of Satureja spicigera and Its Main Compounds |
| title_sort | evaluation of the cytotoxicity of satureja spicigera and its main compounds |
| url | http://dx.doi.org/10.1100/2012/203861 |
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