Comparative miRNome analysis of colostrum- and mature milk–derived extracellular vesicles from Holstein and Jersey cows
MicroRNAs (miRNAs) are small noncoding RNAs that play a pivotal role in the regulation of gene expression. Analysis of miRNAs is important for understanding a variety of biological processes. Sequencing of miRNAs within milk-derived extracellular vesicles (EVs) provides valuable insights into the mo...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Korean Society of Animal Sciences and Technology
2025-01-01
|
| Series: | Journal of Animal Science and Technology |
| Subjects: | |
| Online Access: | http://www.ejast.org/archive/view_article?doi=10.5187/jast.2024.e84 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850024349813178368 |
|---|---|
| author | Daye Mun Sangdon Ryu Dong-Hyun Lim Sangnam Oh Younghoon Kim |
| author_facet | Daye Mun Sangdon Ryu Dong-Hyun Lim Sangnam Oh Younghoon Kim |
| author_sort | Daye Mun |
| collection | DOAJ |
| description | MicroRNAs (miRNAs) are small noncoding RNAs that play a pivotal role in the regulation of gene expression. Analysis of miRNAs is important for understanding a variety of biological processes. Sequencing of miRNAs within milk-derived extracellular vesicles (EVs) provides valuable insights into the molecular mechanisms through which these EVs influence recipient cells. Comparative miRNA sequencing of colostrum and mature milk from different cow breeds can demonstrate breed-specific differences and improve the understanding of potential therapeutic applications in immune regulation and gut health. Therefore, this study was conducted to compare the miRNA profiles and characteristics of colostrum- and mature milk–derived EVs from Holstein and Jersey breeds and determine their effects on intestinal epithelial cells. The miRNA profiles of EVs isolated from the colostrum and mature milk of Holstein and Jersey cows were analyzed via small RNA sequencing. Holstein colostrum–derived EVs exhibited the most diverse miRNA profile with 421 identified miRNAs compared with 259 in mature milk–derived EVs. Jersey colostrum EVs had 198 miRNAs, whereas mature milk EVs had 282. Differential expression analysis revealed considerable miRNA differences between colostrum and mature milk, particularly in Holstein cows. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses revealed that miRNAs from colostrum EVs predominantly regulated immune-related pathways. Transcriptomic analysis of human colon cell line HT-29 treated with Holstein colostrum EVs confirmed the modulation of genes associated with immune responses. These findings indicate that colostrum-derived EVs, particularly from Holstein cows, play a pivotal role in immune regulation and could be potential candidates for therapeutic applications. |
| format | Article |
| id | doaj-art-629e5dc16cfa4e4bbc583d3ff82efecc |
| institution | DOAJ |
| issn | 2672-0191 2055-0391 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Korean Society of Animal Sciences and Technology |
| record_format | Article |
| series | Journal of Animal Science and Technology |
| spelling | doaj-art-629e5dc16cfa4e4bbc583d3ff82efecc2025-08-20T03:01:07ZengKorean Society of Animal Sciences and TechnologyJournal of Animal Science and Technology2672-01912055-03912025-01-0167119320710.5187/jast.2024.e84Comparative miRNome analysis of colostrum- and mature milk–derived extracellular vesicles from Holstein and Jersey cowsDaye Mun0Sangdon Ryu1Dong-Hyun Lim2Sangnam Oh3Younghoon Kim4Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, KoreaHonam National Institute of Biological Resources, Mokpo 58762, KoreaDairy Science Division, National Institute of Animal Science, Rural Development Administration, Cheonan 31000, KoreaDepartment of Functional Food and Biotechnology, Jeonju University, Jeonju 55069, KoreaDepartment of Agricultural Biotechnology and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, KoreaMicroRNAs (miRNAs) are small noncoding RNAs that play a pivotal role in the regulation of gene expression. Analysis of miRNAs is important for understanding a variety of biological processes. Sequencing of miRNAs within milk-derived extracellular vesicles (EVs) provides valuable insights into the molecular mechanisms through which these EVs influence recipient cells. Comparative miRNA sequencing of colostrum and mature milk from different cow breeds can demonstrate breed-specific differences and improve the understanding of potential therapeutic applications in immune regulation and gut health. Therefore, this study was conducted to compare the miRNA profiles and characteristics of colostrum- and mature milk–derived EVs from Holstein and Jersey breeds and determine their effects on intestinal epithelial cells. The miRNA profiles of EVs isolated from the colostrum and mature milk of Holstein and Jersey cows were analyzed via small RNA sequencing. Holstein colostrum–derived EVs exhibited the most diverse miRNA profile with 421 identified miRNAs compared with 259 in mature milk–derived EVs. Jersey colostrum EVs had 198 miRNAs, whereas mature milk EVs had 282. Differential expression analysis revealed considerable miRNA differences between colostrum and mature milk, particularly in Holstein cows. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses revealed that miRNAs from colostrum EVs predominantly regulated immune-related pathways. Transcriptomic analysis of human colon cell line HT-29 treated with Holstein colostrum EVs confirmed the modulation of genes associated with immune responses. These findings indicate that colostrum-derived EVs, particularly from Holstein cows, play a pivotal role in immune regulation and could be potential candidates for therapeutic applications. http://www.ejast.org/archive/view_article?doi=10.5187/jast.2024.e84MicroRNAsExtracellular vesiclesHolsteinJerseyColostrumMature milk |
| spellingShingle | Daye Mun Sangdon Ryu Dong-Hyun Lim Sangnam Oh Younghoon Kim Comparative miRNome analysis of colostrum- and mature milk–derived extracellular vesicles from Holstein and Jersey cows Journal of Animal Science and Technology MicroRNAs Extracellular vesicles Holstein Jersey Colostrum Mature milk |
| title | Comparative miRNome analysis of colostrum- and mature milk–derived extracellular vesicles from Holstein and Jersey cows |
| title_full | Comparative miRNome analysis of colostrum- and mature milk–derived extracellular vesicles from Holstein and Jersey cows |
| title_fullStr | Comparative miRNome analysis of colostrum- and mature milk–derived extracellular vesicles from Holstein and Jersey cows |
| title_full_unstemmed | Comparative miRNome analysis of colostrum- and mature milk–derived extracellular vesicles from Holstein and Jersey cows |
| title_short | Comparative miRNome analysis of colostrum- and mature milk–derived extracellular vesicles from Holstein and Jersey cows |
| title_sort | comparative mirnome analysis of colostrum and mature milk derived extracellular vesicles from holstein and jersey cows |
| topic | MicroRNAs Extracellular vesicles Holstein Jersey Colostrum Mature milk |
| url | http://www.ejast.org/archive/view_article?doi=10.5187/jast.2024.e84 |
| work_keys_str_mv | AT dayemun comparativemirnomeanalysisofcolostrumandmaturemilkderivedextracellularvesiclesfromholsteinandjerseycows AT sangdonryu comparativemirnomeanalysisofcolostrumandmaturemilkderivedextracellularvesiclesfromholsteinandjerseycows AT donghyunlim comparativemirnomeanalysisofcolostrumandmaturemilkderivedextracellularvesiclesfromholsteinandjerseycows AT sangnamoh comparativemirnomeanalysisofcolostrumandmaturemilkderivedextracellularvesiclesfromholsteinandjerseycows AT younghoonkim comparativemirnomeanalysisofcolostrumandmaturemilkderivedextracellularvesiclesfromholsteinandjerseycows |