Allergic Responses Induced by a Fungal Biopesticide Metarhizium anisopliae and House Dust Mite Are Compared in a Mouse Model
Biopesticides can be effective in controlling their target pest. However, research regarding allergenicity and asthma development is limited. We compared the ability of fungal biopesticide Metarhizium anisopliae (MACA) and house dust mite (HDM) extracts to induce allergic responses in BALB/c mice....
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2011-01-01
|
| Series: | Journal of Toxicology |
| Online Access: | http://dx.doi.org/10.1155/2011/360805 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832566679926734848 |
|---|---|
| author | Marsha D. W. Ward Yong Joo Chung Lisa B. Copeland Donald L. Doerfler |
| author_facet | Marsha D. W. Ward Yong Joo Chung Lisa B. Copeland Donald L. Doerfler |
| author_sort | Marsha D. W. Ward |
| collection | DOAJ |
| description | Biopesticides can be effective in controlling their target pest. However, research regarding allergenicity and asthma development is limited. We compared the ability of fungal biopesticide Metarhizium anisopliae (MACA) and house dust mite (HDM) extracts to induce allergic responses in BALB/c mice. The extracts were administered by intratracheal aspiration at doubling doses (2.5–80 𝜇g protein) 4X over a four-week period. Three days after the last exposure, serum and bronchoalveolar lavage fluid (BALF) were collected. The extracts' relative allergenicity was evaluated based on response robustness (lowest significant dose response compared to control (0 𝜇g)). MACA induced a more robust serum total IgE response than HDM. However, in the antigen-specific IgE assay, a similar dose of both MACA and HDM was required to achieve the same response level. Our data suggest a threshold dose of MACA for allergy induction and that M. anisopliae may be similar to HDM in allergy induction potential. |
| format | Article |
| id | doaj-art-6295a6b217b5469bbcc82708706e82e6 |
| institution | Kabale University |
| issn | 1687-8191 1687-8205 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Toxicology |
| spelling | doaj-art-6295a6b217b5469bbcc82708706e82e62025-02-03T01:03:30ZengWileyJournal of Toxicology1687-81911687-82052011-01-01201110.1155/2011/360805360805Allergic Responses Induced by a Fungal Biopesticide Metarhizium anisopliae and House Dust Mite Are Compared in a Mouse ModelMarsha D. W. Ward0Yong Joo Chung1Lisa B. Copeland2Donald L. Doerfler3National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, 109 T. W. Alexander Drive, MD B105-02, Research Triangle Park, NC 27711, USANational Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, 109 T. W. Alexander Drive, MD B105-02, Research Triangle Park, NC 27711, USANational Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, 109 T. W. Alexander Drive, MD B105-02, Research Triangle Park, NC 27711, USANational Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, 109 T. W. Alexander Drive, MD B105-02, Research Triangle Park, NC 27711, USABiopesticides can be effective in controlling their target pest. However, research regarding allergenicity and asthma development is limited. We compared the ability of fungal biopesticide Metarhizium anisopliae (MACA) and house dust mite (HDM) extracts to induce allergic responses in BALB/c mice. The extracts were administered by intratracheal aspiration at doubling doses (2.5–80 𝜇g protein) 4X over a four-week period. Three days after the last exposure, serum and bronchoalveolar lavage fluid (BALF) were collected. The extracts' relative allergenicity was evaluated based on response robustness (lowest significant dose response compared to control (0 𝜇g)). MACA induced a more robust serum total IgE response than HDM. However, in the antigen-specific IgE assay, a similar dose of both MACA and HDM was required to achieve the same response level. Our data suggest a threshold dose of MACA for allergy induction and that M. anisopliae may be similar to HDM in allergy induction potential.http://dx.doi.org/10.1155/2011/360805 |
| spellingShingle | Marsha D. W. Ward Yong Joo Chung Lisa B. Copeland Donald L. Doerfler Allergic Responses Induced by a Fungal Biopesticide Metarhizium anisopliae and House Dust Mite Are Compared in a Mouse Model Journal of Toxicology |
| title | Allergic Responses Induced by a Fungal Biopesticide Metarhizium anisopliae and House Dust Mite Are Compared in a Mouse Model |
| title_full | Allergic Responses Induced by a Fungal Biopesticide Metarhizium anisopliae and House Dust Mite Are Compared in a Mouse Model |
| title_fullStr | Allergic Responses Induced by a Fungal Biopesticide Metarhizium anisopliae and House Dust Mite Are Compared in a Mouse Model |
| title_full_unstemmed | Allergic Responses Induced by a Fungal Biopesticide Metarhizium anisopliae and House Dust Mite Are Compared in a Mouse Model |
| title_short | Allergic Responses Induced by a Fungal Biopesticide Metarhizium anisopliae and House Dust Mite Are Compared in a Mouse Model |
| title_sort | allergic responses induced by a fungal biopesticide metarhizium anisopliae and house dust mite are compared in a mouse model |
| url | http://dx.doi.org/10.1155/2011/360805 |
| work_keys_str_mv | AT marshadwward allergicresponsesinducedbyafungalbiopesticidemetarhiziumanisopliaeandhousedustmitearecomparedinamousemodel AT yongjoochung allergicresponsesinducedbyafungalbiopesticidemetarhiziumanisopliaeandhousedustmitearecomparedinamousemodel AT lisabcopeland allergicresponsesinducedbyafungalbiopesticidemetarhiziumanisopliaeandhousedustmitearecomparedinamousemodel AT donaldldoerfler allergicresponsesinducedbyafungalbiopesticidemetarhiziumanisopliaeandhousedustmitearecomparedinamousemodel |