The effect of interferon alpha administration on cytokine levels in sheep
<b>Aim: </b>The primary aim of this study was to determine the effect of parenteral recombinant human interferon (rHuIFN)-?2a administration on serum tumor necrosis factor (TNF)-?, interleukin (IL)-6 and IL-10 levels in healthy sheep. In addition, the main physiological parameters (recta...
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| Format: | Article |
| Language: | English |
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Selcuk University Press
2020-12-01
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| Series: | Eurasian Journal of Veterinary Sciences |
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| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1336 |
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| author | Devran Coşkun Merve İder Mustafa Sedat Arslan Rahmi Canbar Enver Yazar |
| author_facet | Devran Coşkun Merve İder Mustafa Sedat Arslan Rahmi Canbar Enver Yazar |
| author_sort | Devran Coşkun |
| collection | DOAJ |
| description | <b>Aim: </b>The primary aim of this study was to determine the effect of
parenteral recombinant human interferon (rHuIFN)-?2a administration
on serum tumor necrosis factor (TNF)-?, interleukin (IL)-6
and IL-10 levels in healthy sheep. In addition, the main physiological
parameters (rectal temperature, pulse rate, respiratory rate), hemogram
and blood gas parameters were determined, as well.<p>
<b>Materials and Methods: </b>In this study, 9.000.000 IU rHuIFN-?2a
was administered subcutaneously in 10 Merinos sheep. Blood samples
were taken before (0 hours) and after at 4, 8, 12, 24, 48, 72, 96
and 120 hours. Rectal temperature, pulse and respiratory rate were
also determined at the same sampling times. Sheep-specific TNF-?,
IL-6 and IL-10 levels were determined from serum samples by the
ELISA reader. Hemogram and blood gas parameters were measured
by complete blood cell counter and blood gas analyzer, respectively.<p>
<b>Results: </b>After rHuIFN-?2a treatment, TNF-? and IL-10 concentrations
reached peak levels (p<0.05) at the 96 hours, whereas the IL-6
level did not change in a statistically significant (p>0.05). On the other
hand, a temporary increase in rectal temperature and pO2 levels
(p<0.05) were determined, while decreased potassium and ionized
calcium levels (p<0.05) were measured. Statistically significantly
(p<0.05) fluctuations were determined in pulse rate, white blood
cell counts, pH, base(ecf) and sodium values.<p>
<b>Conclusion:</b> It may be stated that administration of rHuIFN-?2a
showed immunological effects in sheep, as it is generally accepted as
safe and can be considered for use in treatment. |
| format | Article |
| id | doaj-art-628418b65fb14c60a0fcda28f33da5a8 |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publishDate | 2020-12-01 |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-628418b65fb14c60a0fcda28f33da5a82025-02-03T11:25:38ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19532020-12-013643243301336The effect of interferon alpha administration on cytokine levels in sheepDevran Coşkun0Merve İder1Mustafa Sedat Arslan2Rahmi Canbar3Enver Yazar4Selcuk University, Veterinary Faculty, Department of Pharmacology and Toxicology, Konya, TurkeySelcuk University, Veterinary Faculty, Department of Internal Medicine, Konya, TurkeySelcuk University, Veterinary Faculty, Prof. Dr. Hümeyra Özgen Research and Application Farm, Konya, TurkeySelcuk University, Veterinary Faculty, Department of Pharmacology and Toxicology, Konya, TurkeySelcuk University, Veterinary Faculty, Department of Pharmacology and Toxicology, Konya, Turkey<b>Aim: </b>The primary aim of this study was to determine the effect of parenteral recombinant human interferon (rHuIFN)-?2a administration on serum tumor necrosis factor (TNF)-?, interleukin (IL)-6 and IL-10 levels in healthy sheep. In addition, the main physiological parameters (rectal temperature, pulse rate, respiratory rate), hemogram and blood gas parameters were determined, as well.<p> <b>Materials and Methods: </b>In this study, 9.000.000 IU rHuIFN-?2a was administered subcutaneously in 10 Merinos sheep. Blood samples were taken before (0 hours) and after at 4, 8, 12, 24, 48, 72, 96 and 120 hours. Rectal temperature, pulse and respiratory rate were also determined at the same sampling times. Sheep-specific TNF-?, IL-6 and IL-10 levels were determined from serum samples by the ELISA reader. Hemogram and blood gas parameters were measured by complete blood cell counter and blood gas analyzer, respectively.<p> <b>Results: </b>After rHuIFN-?2a treatment, TNF-? and IL-10 concentrations reached peak levels (p<0.05) at the 96 hours, whereas the IL-6 level did not change in a statistically significant (p>0.05). On the other hand, a temporary increase in rectal temperature and pO2 levels (p<0.05) were determined, while decreased potassium and ionized calcium levels (p<0.05) were measured. Statistically significantly (p<0.05) fluctuations were determined in pulse rate, white blood cell counts, pH, base(ecf) and sodium values.<p> <b>Conclusion:</b> It may be stated that administration of rHuIFN-?2a showed immunological effects in sheep, as it is generally accepted as safe and can be considered for use in treatment.http://eurasianjvetsci.org/pdf.php3?id=1336sheepinterferoncytokineshemogramblood gases |
| spellingShingle | Devran Coşkun Merve İder Mustafa Sedat Arslan Rahmi Canbar Enver Yazar The effect of interferon alpha administration on cytokine levels in sheep Eurasian Journal of Veterinary Sciences sheep interferon cytokines hemogram blood gases |
| title | The effect of interferon alpha administration on cytokine levels in sheep |
| title_full | The effect of interferon alpha administration on cytokine levels in sheep |
| title_fullStr | The effect of interferon alpha administration on cytokine levels in sheep |
| title_full_unstemmed | The effect of interferon alpha administration on cytokine levels in sheep |
| title_short | The effect of interferon alpha administration on cytokine levels in sheep |
| title_sort | effect of interferon alpha administration on cytokine levels in sheep |
| topic | sheep interferon cytokines hemogram blood gases |
| url | http://eurasianjvetsci.org/pdf.php3?id=1336 |
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