The Effect of Mycobacterium avium Complex Infections on Routine Mycobacterium bovis Diagnostic Tests
Bovine tuberculosis (bTB) is diagnosed in naturally infected populations exposed to a wide variety of other pathogens. This study describes the cell-mediated immune responses of cattle exposed to Mycobacterium avium subspecies paratuberculosis (Map) and Mycobacterium avium subspecies avium with part...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2011-01-01
|
| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.4061/2011/145092 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832555469345914880 |
|---|---|
| author | Claire Barry David Corbett Douwe Bakker Peter Andersen Jim McNair Sam Strain |
| author_facet | Claire Barry David Corbett Douwe Bakker Peter Andersen Jim McNair Sam Strain |
| author_sort | Claire Barry |
| collection | DOAJ |
| description | Bovine tuberculosis (bTB) is diagnosed in naturally infected populations exposed to a wide variety of other pathogens. This study describes the cell-mediated immune responses of cattle exposed to Mycobacterium avium subspecies paratuberculosis (Map) and Mycobacterium avium subspecies avium with particular reference to routine antefmortem Mycobacterium bovis diagnostic tests. The IFN-γ released in response to stimulated blood was found to peak later in the Map-exposed group and was more sustained when compared to the Maa-exposed group. There was a very close correlation between the responses to the purified protein derivatives (PPD) used for stimulation (PPDa, PPDb, and PPDj) with PPDa and PPDj most closely correlated. On occasion, in the Map-infected cattle, PPDb-biased responses were seen compared to PPDa suggesting that some Map-infected cattle could be misclassified as M. bovis infected using this test with these reagents. This bias was not seen when PPDj was used. SICCT results were consistent with the respective infections and all calves would have been classed skin test negative. |
| format | Article |
| id | doaj-art-6260bd9f245f47b593e717c6caa28e7f |
| institution | Kabale University |
| issn | 2042-0048 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-6260bd9f245f47b593e717c6caa28e7f2025-02-03T05:48:08ZengWileyVeterinary Medicine International2042-00482011-01-01201110.4061/2011/145092145092The Effect of Mycobacterium avium Complex Infections on Routine Mycobacterium bovis Diagnostic TestsClaire Barry0David Corbett1Douwe Bakker2Peter Andersen3Jim McNair4Sam Strain5Veterinary Sciences Division, Agri-Food and Biosciences Institute, Stoney Road, Stormont, Belfast BT4 3SD, Northern IrelandVeterinary Sciences Division, Agri-Food and Biosciences Institute, Stoney Road, Stormont, Belfast BT4 3SD, Northern IrelandCentral Veterinary Institute, P.O. Box 65, Edelhertweg 15, 8200 AB Lelystad, The NetherlandsDepartment of Infectious Immunology, Statens Serum Institut, Artillerivej, 2300 Copenhagen, DenmarkVeterinary Sciences Division, Agri-Food and Biosciences Institute, Stoney Road, Stormont, Belfast BT4 3SD, Northern IrelandVeterinary Sciences Division, Agri-Food and Biosciences Institute, Stoney Road, Stormont, Belfast BT4 3SD, Northern IrelandBovine tuberculosis (bTB) is diagnosed in naturally infected populations exposed to a wide variety of other pathogens. This study describes the cell-mediated immune responses of cattle exposed to Mycobacterium avium subspecies paratuberculosis (Map) and Mycobacterium avium subspecies avium with particular reference to routine antefmortem Mycobacterium bovis diagnostic tests. The IFN-γ released in response to stimulated blood was found to peak later in the Map-exposed group and was more sustained when compared to the Maa-exposed group. There was a very close correlation between the responses to the purified protein derivatives (PPD) used for stimulation (PPDa, PPDb, and PPDj) with PPDa and PPDj most closely correlated. On occasion, in the Map-infected cattle, PPDb-biased responses were seen compared to PPDa suggesting that some Map-infected cattle could be misclassified as M. bovis infected using this test with these reagents. This bias was not seen when PPDj was used. SICCT results were consistent with the respective infections and all calves would have been classed skin test negative.http://dx.doi.org/10.4061/2011/145092 |
| spellingShingle | Claire Barry David Corbett Douwe Bakker Peter Andersen Jim McNair Sam Strain The Effect of Mycobacterium avium Complex Infections on Routine Mycobacterium bovis Diagnostic Tests Veterinary Medicine International |
| title | The Effect of Mycobacterium avium Complex Infections on Routine Mycobacterium bovis Diagnostic Tests |
| title_full | The Effect of Mycobacterium avium Complex Infections on Routine Mycobacterium bovis Diagnostic Tests |
| title_fullStr | The Effect of Mycobacterium avium Complex Infections on Routine Mycobacterium bovis Diagnostic Tests |
| title_full_unstemmed | The Effect of Mycobacterium avium Complex Infections on Routine Mycobacterium bovis Diagnostic Tests |
| title_short | The Effect of Mycobacterium avium Complex Infections on Routine Mycobacterium bovis Diagnostic Tests |
| title_sort | effect of mycobacterium avium complex infections on routine mycobacterium bovis diagnostic tests |
| url | http://dx.doi.org/10.4061/2011/145092 |
| work_keys_str_mv | AT clairebarry theeffectofmycobacteriumaviumcomplexinfectionsonroutinemycobacteriumbovisdiagnostictests AT davidcorbett theeffectofmycobacteriumaviumcomplexinfectionsonroutinemycobacteriumbovisdiagnostictests AT douwebakker theeffectofmycobacteriumaviumcomplexinfectionsonroutinemycobacteriumbovisdiagnostictests AT peterandersen theeffectofmycobacteriumaviumcomplexinfectionsonroutinemycobacteriumbovisdiagnostictests AT jimmcnair theeffectofmycobacteriumaviumcomplexinfectionsonroutinemycobacteriumbovisdiagnostictests AT samstrain theeffectofmycobacteriumaviumcomplexinfectionsonroutinemycobacteriumbovisdiagnostictests AT clairebarry effectofmycobacteriumaviumcomplexinfectionsonroutinemycobacteriumbovisdiagnostictests AT davidcorbett effectofmycobacteriumaviumcomplexinfectionsonroutinemycobacteriumbovisdiagnostictests AT douwebakker effectofmycobacteriumaviumcomplexinfectionsonroutinemycobacteriumbovisdiagnostictests AT peterandersen effectofmycobacteriumaviumcomplexinfectionsonroutinemycobacteriumbovisdiagnostictests AT jimmcnair effectofmycobacteriumaviumcomplexinfectionsonroutinemycobacteriumbovisdiagnostictests AT samstrain effectofmycobacteriumaviumcomplexinfectionsonroutinemycobacteriumbovisdiagnostictests |