lncRNA HHIP-AS1 Promotes the Osteogenic Differentiation Potential and Inhibits the Migration Ability of Periodontal Ligament Stem Cells
Alveolar bone remodeling under orthodontic force is achieved by periodontal ligament stem cells (PDLSCs), which are sensitive to mechanical loading. How to regulate functions of PDLSCs is a key issue in bone remodeling during orthodontic tooth movement. This study is aimed at investigating the roles...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2021/5595580 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832561641076555776 |
|---|---|
| author | Qianyi Qin Haoqing Yang Chen Zhang Xiao Han Jing Guo Zhipeng Fan Jie Guo |
| author_facet | Qianyi Qin Haoqing Yang Chen Zhang Xiao Han Jing Guo Zhipeng Fan Jie Guo |
| author_sort | Qianyi Qin |
| collection | DOAJ |
| description | Alveolar bone remodeling under orthodontic force is achieved by periodontal ligament stem cells (PDLSCs), which are sensitive to mechanical loading. How to regulate functions of PDLSCs is a key issue in bone remodeling during orthodontic tooth movement. This study is aimed at investigating the roles of lncRNA Hedgehog-interacting protein antisense RNA 1 (HHIP-AS1) in the functional regulation of PDLSCs. First, HHIP-AS1 expression was downregulated in PDLSCs under continuous compressive pressure. Then, we found that the alkaline phosphatase activity, in vitro mineralization, and expression levels of bone sialoprotein, osteocalcin, and osterix were increased in PDLSCs by HHIP-AS1. The results of scratch migration and transwell chemotaxis assays revealed that HHIP-AS1 inhibited the migration and chemotaxis abilities of PDLSCs. In addition, the RNA sequencing data showed that 356 mRNAs and 14 lncRNAs were upregulated, including receptor tyrosine kinase-like orphan receptor 2 and nuclear-enriched abundant transcript 1, while 185 mRNAs and 6 lncRNAs were downregulated, including fibroblast growth factor 5 and LINC00973, in HHIP-AS1-depleted PDLSCs. Bioinformatic analysis revealed several biological processes and signaling pathways related to HHIP-AS1 functions, including the PI3K-Akt signaling pathway and JAK-STAT signaling pathway. In conclusion, our findings indicated that HHIP-AS1 was downregulated in PDLSCs under compressive pressure, and it promoted the osteogenic differentiation potential and inhibited the migration and chemotaxis abilities of PDLSCs. Thus, HHIP-AS1 may be a potential target for accelerating tooth movement during orthodontic treatment. |
| format | Article |
| id | doaj-art-625eef6f60f945dfa965781a079a0d4e |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-625eef6f60f945dfa965781a079a0d4e2025-02-03T01:24:31ZengWileyStem Cells International1687-966X1687-96782021-01-01202110.1155/2021/55955805595580lncRNA HHIP-AS1 Promotes the Osteogenic Differentiation Potential and Inhibits the Migration Ability of Periodontal Ligament Stem CellsQianyi Qin0Haoqing Yang1Chen Zhang2Xiao Han3Jing Guo4Zhipeng Fan5Jie Guo6Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University, Shandong Key Laboratory of Oral Tissue Regeneration, and Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, 250012, ChinaLaboratory of Molecular Signaling and Stem Cells Therapy, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical University, 100050, ChinaLaboratory of Molecular Signaling and Stem Cells Therapy, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical University, 100050, ChinaLaboratory of Molecular Signaling and Stem Cells Therapy, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical University, 100050, ChinaThe Affiliated Stomatological Hospital of Nanchang University and the Key Laboratory of Oral Biomedicine, Jiangxi Province 330006, ChinaLaboratory of Molecular Signaling and Stem Cells Therapy, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical University, 100050, ChinaDepartment of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University, Shandong Key Laboratory of Oral Tissue Regeneration, and Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, 250012, ChinaAlveolar bone remodeling under orthodontic force is achieved by periodontal ligament stem cells (PDLSCs), which are sensitive to mechanical loading. How to regulate functions of PDLSCs is a key issue in bone remodeling during orthodontic tooth movement. This study is aimed at investigating the roles of lncRNA Hedgehog-interacting protein antisense RNA 1 (HHIP-AS1) in the functional regulation of PDLSCs. First, HHIP-AS1 expression was downregulated in PDLSCs under continuous compressive pressure. Then, we found that the alkaline phosphatase activity, in vitro mineralization, and expression levels of bone sialoprotein, osteocalcin, and osterix were increased in PDLSCs by HHIP-AS1. The results of scratch migration and transwell chemotaxis assays revealed that HHIP-AS1 inhibited the migration and chemotaxis abilities of PDLSCs. In addition, the RNA sequencing data showed that 356 mRNAs and 14 lncRNAs were upregulated, including receptor tyrosine kinase-like orphan receptor 2 and nuclear-enriched abundant transcript 1, while 185 mRNAs and 6 lncRNAs were downregulated, including fibroblast growth factor 5 and LINC00973, in HHIP-AS1-depleted PDLSCs. Bioinformatic analysis revealed several biological processes and signaling pathways related to HHIP-AS1 functions, including the PI3K-Akt signaling pathway and JAK-STAT signaling pathway. In conclusion, our findings indicated that HHIP-AS1 was downregulated in PDLSCs under compressive pressure, and it promoted the osteogenic differentiation potential and inhibited the migration and chemotaxis abilities of PDLSCs. Thus, HHIP-AS1 may be a potential target for accelerating tooth movement during orthodontic treatment.http://dx.doi.org/10.1155/2021/5595580 |
| spellingShingle | Qianyi Qin Haoqing Yang Chen Zhang Xiao Han Jing Guo Zhipeng Fan Jie Guo lncRNA HHIP-AS1 Promotes the Osteogenic Differentiation Potential and Inhibits the Migration Ability of Periodontal Ligament Stem Cells Stem Cells International |
| title | lncRNA HHIP-AS1 Promotes the Osteogenic Differentiation Potential and Inhibits the Migration Ability of Periodontal Ligament Stem Cells |
| title_full | lncRNA HHIP-AS1 Promotes the Osteogenic Differentiation Potential and Inhibits the Migration Ability of Periodontal Ligament Stem Cells |
| title_fullStr | lncRNA HHIP-AS1 Promotes the Osteogenic Differentiation Potential and Inhibits the Migration Ability of Periodontal Ligament Stem Cells |
| title_full_unstemmed | lncRNA HHIP-AS1 Promotes the Osteogenic Differentiation Potential and Inhibits the Migration Ability of Periodontal Ligament Stem Cells |
| title_short | lncRNA HHIP-AS1 Promotes the Osteogenic Differentiation Potential and Inhibits the Migration Ability of Periodontal Ligament Stem Cells |
| title_sort | lncrna hhip as1 promotes the osteogenic differentiation potential and inhibits the migration ability of periodontal ligament stem cells |
| url | http://dx.doi.org/10.1155/2021/5595580 |
| work_keys_str_mv | AT qianyiqin lncrnahhipas1promotestheosteogenicdifferentiationpotentialandinhibitsthemigrationabilityofperiodontalligamentstemcells AT haoqingyang lncrnahhipas1promotestheosteogenicdifferentiationpotentialandinhibitsthemigrationabilityofperiodontalligamentstemcells AT chenzhang lncrnahhipas1promotestheosteogenicdifferentiationpotentialandinhibitsthemigrationabilityofperiodontalligamentstemcells AT xiaohan lncrnahhipas1promotestheosteogenicdifferentiationpotentialandinhibitsthemigrationabilityofperiodontalligamentstemcells AT jingguo lncrnahhipas1promotestheosteogenicdifferentiationpotentialandinhibitsthemigrationabilityofperiodontalligamentstemcells AT zhipengfan lncrnahhipas1promotestheosteogenicdifferentiationpotentialandinhibitsthemigrationabilityofperiodontalligamentstemcells AT jieguo lncrnahhipas1promotestheosteogenicdifferentiationpotentialandinhibitsthemigrationabilityofperiodontalligamentstemcells |