Comparison of Vaccination Regimens on Immune Responses Using PED Replicon Vaccine: A Field Trial in PED-Negative and PED-Positive Thai Swine Farms
Porcine epidemic diarrhea virus (PEDV) is an economically important pathogen of swine, causing severe diarrhea in neonates with high morbidity and mortality. Vaccination is a key strategy for PEDV control, but optimizing regimens based on herd status is essential for improving immunity and protectio...
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2025-01-01
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| author | Chaitawat Sirisereewan Thanh Che Nguyen Nanthiya Iampraphat Hongyao Lin Leonardo Ellerma Pisit Sirithanyakul Roongtham Kedkovid Roongroje Thanawongnuwech |
| author_facet | Chaitawat Sirisereewan Thanh Che Nguyen Nanthiya Iampraphat Hongyao Lin Leonardo Ellerma Pisit Sirithanyakul Roongtham Kedkovid Roongroje Thanawongnuwech |
| author_sort | Chaitawat Sirisereewan |
| collection | DOAJ |
| description | Porcine epidemic diarrhea virus (PEDV) is an economically important pathogen of swine, causing severe diarrhea in neonates with high morbidity and mortality. Vaccination is a key strategy for PEDV control, but optimizing regimens based on herd status is essential for improving immunity and protection. This study evaluated immune responses to different vaccination protocols using a PED replicon vaccine (PED-RP) in Thai swine farms with varying PED statuses. Two field trials were conducted: Experiment 1 (<i>n</i> = 120) on a PED-negative farm used a killed vaccine followed by PED-RP, while Experiment 2 (<i>n</i> = 42) on a PED-positive farm used a modified-live vaccine (MLV) followed by PED-RP. In Experiment 1, gilts boosted with PED-RP during gestation showed significantly higher colostrum IgA levels compared to those receiving two doses of the killed vaccine. In Experiment 2, priming with MLV followed by PED-RP resulted in increased neutralizing antibody titers and seropositivity, outperforming the MLV-only regimen after acclimatization. These findings suggest that using PED-RP as a booster can enhance serum and lactogenic immunity, providing a promising strategy for improving immune responses in both PED-negative and PED-positive herds. The study highlights the importance of tailored vaccination strategies to optimize herd immunity and protect piglets from PEDV. |
| format | Article |
| id | doaj-art-61e6e216c5424ba9afdd1f1f5ad33931 |
| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj-art-61e6e216c5424ba9afdd1f1f5ad339312025-01-24T13:18:23ZengMDPI AGAnimals2076-26152025-01-0115227310.3390/ani15020273Comparison of Vaccination Regimens on Immune Responses Using PED Replicon Vaccine: A Field Trial in PED-Negative and PED-Positive Thai Swine FarmsChaitawat Sirisereewan0Thanh Che Nguyen1Nanthiya Iampraphat2Hongyao Lin3Leonardo Ellerma4Pisit Sirithanyakul5Roongtham Kedkovid6Roongroje Thanawongnuwech7Department of Veterinary Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Veterinary Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandVeterinary Diagnostic Laboratory, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandMSD Animal Health Innovation Pte Ltd., Perahu Road, Singapore 718847, SingaporeMSD Animal Health (Phils) Inc., 8767 Paseo De Roxas, 26/F AIA Tower, Makati 1200, PhilippinesIntervet Thailand Ltd., 999/9 The Offices at Central World, 37th Rama 1 Road, Pathumwan, Bangkok 10330, ThailandCenter of Excellence for Emerging and Re-emerging Infectious Diseases in Animals and One Health Research Cluster, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Veterinary Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, ThailandPorcine epidemic diarrhea virus (PEDV) is an economically important pathogen of swine, causing severe diarrhea in neonates with high morbidity and mortality. Vaccination is a key strategy for PEDV control, but optimizing regimens based on herd status is essential for improving immunity and protection. This study evaluated immune responses to different vaccination protocols using a PED replicon vaccine (PED-RP) in Thai swine farms with varying PED statuses. Two field trials were conducted: Experiment 1 (<i>n</i> = 120) on a PED-negative farm used a killed vaccine followed by PED-RP, while Experiment 2 (<i>n</i> = 42) on a PED-positive farm used a modified-live vaccine (MLV) followed by PED-RP. In Experiment 1, gilts boosted with PED-RP during gestation showed significantly higher colostrum IgA levels compared to those receiving two doses of the killed vaccine. In Experiment 2, priming with MLV followed by PED-RP resulted in increased neutralizing antibody titers and seropositivity, outperforming the MLV-only regimen after acclimatization. These findings suggest that using PED-RP as a booster can enhance serum and lactogenic immunity, providing a promising strategy for improving immune responses in both PED-negative and PED-positive herds. The study highlights the importance of tailored vaccination strategies to optimize herd immunity and protect piglets from PEDV.https://www.mdpi.com/2076-2615/15/2/273porcine epidemic diarrheaporcine epidemic diarrhea virusreplicon vaccinepigvaccination |
| spellingShingle | Chaitawat Sirisereewan Thanh Che Nguyen Nanthiya Iampraphat Hongyao Lin Leonardo Ellerma Pisit Sirithanyakul Roongtham Kedkovid Roongroje Thanawongnuwech Comparison of Vaccination Regimens on Immune Responses Using PED Replicon Vaccine: A Field Trial in PED-Negative and PED-Positive Thai Swine Farms Animals porcine epidemic diarrhea porcine epidemic diarrhea virus replicon vaccine pig vaccination |
| title | Comparison of Vaccination Regimens on Immune Responses Using PED Replicon Vaccine: A Field Trial in PED-Negative and PED-Positive Thai Swine Farms |
| title_full | Comparison of Vaccination Regimens on Immune Responses Using PED Replicon Vaccine: A Field Trial in PED-Negative and PED-Positive Thai Swine Farms |
| title_fullStr | Comparison of Vaccination Regimens on Immune Responses Using PED Replicon Vaccine: A Field Trial in PED-Negative and PED-Positive Thai Swine Farms |
| title_full_unstemmed | Comparison of Vaccination Regimens on Immune Responses Using PED Replicon Vaccine: A Field Trial in PED-Negative and PED-Positive Thai Swine Farms |
| title_short | Comparison of Vaccination Regimens on Immune Responses Using PED Replicon Vaccine: A Field Trial in PED-Negative and PED-Positive Thai Swine Farms |
| title_sort | comparison of vaccination regimens on immune responses using ped replicon vaccine a field trial in ped negative and ped positive thai swine farms |
| topic | porcine epidemic diarrhea porcine epidemic diarrhea virus replicon vaccine pig vaccination |
| url | https://www.mdpi.com/2076-2615/15/2/273 |
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