The Intoxication Effects of Methanol and Formic Acid on Rat Retina Function
Objective. To explore the potential effects of methanol and its metabolite, formic acid, on rat retina function. Methods. Sprague-Dawley rats were divided into 3- and 7-day groups and a control. Experimental groups were given methanol and the control group were provided saline by gavage. Retinal fun...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2016/4087096 |
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| _version_ | 1832565384068202496 |
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| author | Dong-Mei Liu Shu Zhou Jie-Min Chen Shu-Ya Peng Wen-Tao Xia |
| author_facet | Dong-Mei Liu Shu Zhou Jie-Min Chen Shu-Ya Peng Wen-Tao Xia |
| author_sort | Dong-Mei Liu |
| collection | DOAJ |
| description | Objective. To explore the potential effects of methanol and its metabolite, formic acid, on rat retina function. Methods. Sprague-Dawley rats were divided into 3- and 7-day groups and a control. Experimental groups were given methanol and the control group were provided saline by gavage. Retinal function of each group was assessed by electroretinogram. Concentrations of methanol and formic acid were detected by GC/HS and HPLC, respectively. Results. The a and b amplitudes of methanol treated groups decreased and latent periods delayed in scotopic and photopic ERG recordings. The summed amplitudes of oscillatory potentials (OPs) of groups B and C decreased and the elapsed time delayed. The amplitudes of OS1, OS3, OS4, and OS5 of group B and OS3, OS4, and OS5 of group C decreased compared with the control group. The IPI1 of group B and IPI1-4 of group C were broader compared with the control group and the IPI1-4 and ET of group B were broader than group C. Conclusions. Both of scotopic and photopic retinal functions were impaired by methanol poisoning, and impairment was more serious in the 7-day than in the 3-day group. OPs, especially later OPs and IPI2, were more sensitive to methanol intoxication than other eletroretinogram subcomponents. |
| format | Article |
| id | doaj-art-61c4448e4d9040cf859eaa9e5fa4b7a2 |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-61c4448e4d9040cf859eaa9e5fa4b7a22025-02-03T01:07:54ZengWileyJournal of Ophthalmology2090-004X2090-00582016-01-01201610.1155/2016/40870964087096The Intoxication Effects of Methanol and Formic Acid on Rat Retina FunctionDong-Mei Liu0Shu Zhou1Jie-Min Chen2Shu-Ya Peng3Wen-Tao Xia4Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Institute of Forensic Sciences, Ministry of Justice, Shanghai 200063, ChinaShanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Institute of Forensic Sciences, Ministry of Justice, Shanghai 200063, ChinaShanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Institute of Forensic Sciences, Ministry of Justice, Shanghai 200063, ChinaXinguang Institute of Judicial Expertise, Linyi 276017, ChinaShanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Institute of Forensic Sciences, Ministry of Justice, Shanghai 200063, ChinaObjective. To explore the potential effects of methanol and its metabolite, formic acid, on rat retina function. Methods. Sprague-Dawley rats were divided into 3- and 7-day groups and a control. Experimental groups were given methanol and the control group were provided saline by gavage. Retinal function of each group was assessed by electroretinogram. Concentrations of methanol and formic acid were detected by GC/HS and HPLC, respectively. Results. The a and b amplitudes of methanol treated groups decreased and latent periods delayed in scotopic and photopic ERG recordings. The summed amplitudes of oscillatory potentials (OPs) of groups B and C decreased and the elapsed time delayed. The amplitudes of OS1, OS3, OS4, and OS5 of group B and OS3, OS4, and OS5 of group C decreased compared with the control group. The IPI1 of group B and IPI1-4 of group C were broader compared with the control group and the IPI1-4 and ET of group B were broader than group C. Conclusions. Both of scotopic and photopic retinal functions were impaired by methanol poisoning, and impairment was more serious in the 7-day than in the 3-day group. OPs, especially later OPs and IPI2, were more sensitive to methanol intoxication than other eletroretinogram subcomponents.http://dx.doi.org/10.1155/2016/4087096 |
| spellingShingle | Dong-Mei Liu Shu Zhou Jie-Min Chen Shu-Ya Peng Wen-Tao Xia The Intoxication Effects of Methanol and Formic Acid on Rat Retina Function Journal of Ophthalmology |
| title | The Intoxication Effects of Methanol and Formic Acid on Rat Retina Function |
| title_full | The Intoxication Effects of Methanol and Formic Acid on Rat Retina Function |
| title_fullStr | The Intoxication Effects of Methanol and Formic Acid on Rat Retina Function |
| title_full_unstemmed | The Intoxication Effects of Methanol and Formic Acid on Rat Retina Function |
| title_short | The Intoxication Effects of Methanol and Formic Acid on Rat Retina Function |
| title_sort | intoxication effects of methanol and formic acid on rat retina function |
| url | http://dx.doi.org/10.1155/2016/4087096 |
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