Lawsone encapsulated polylactic‐co‐glycolic acid nanoparticles modified with chitosan‐folic acid successfully inhibited cell growth and triggered apoptosis in Panc‐1 cancer cells
Abstract The present research aims to encapsulate lawsone in polylactic‐co‐glycolic acid (PLGA) nanoparticles modified with folic acid (FA) and chitosan (CS) to study its anticancer effects against Panc‐1 cells. The nanoparticles were analysed in means of shape/size and zeta potential index using sc...
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| Format: | Article |
| Language: | English |
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Wiley
2023-07-01
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| Series: | IET Nanobiotechnology |
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| Online Access: | https://doi.org/10.1049/nbt2.12139 |
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| author | Helia Ghafaripour Masoud Homayouni Tabrizi Ehsan Karimi Niloofar Barati Naeeni |
| author_facet | Helia Ghafaripour Masoud Homayouni Tabrizi Ehsan Karimi Niloofar Barati Naeeni |
| author_sort | Helia Ghafaripour |
| collection | DOAJ |
| description | Abstract The present research aims to encapsulate lawsone in polylactic‐co‐glycolic acid (PLGA) nanoparticles modified with folic acid (FA) and chitosan (CS) to study its anticancer effects against Panc‐1 cells. The nanoparticles were analysed in means of shape/size and zeta potential index using scanning electron microscope and dynamic light scattering. High‐performance liquid chromatography was applied to evaluate the lawsone entrapment efficacy. The authors performed acridine orange/propidium iodide staining and flow cytometry to monitor apoptosis induction and cell cycle arrest. The expressions of apoptosis‐related genes (BAX and BCL‐2) were assessed by real time PCR. Nanoparticle antioxidative and antibacterial activities were examined by DPPH/ABTS scavenging assay, disk diffusion method, and minimum inhibitory concentration and minimum bactericidal concentration evaluation. The NPs were 229.65 nm, the encapsulation efficiency was 81%. The concentration of lawsone that exerts 50% cell growth inhibition (IC50) against Panc‐1 cells was calculated 118.4 μL. Apoptosis induction was evidenced by the increased number of orange cells and increased proportion of cells in G1‐Sub phase respectively. Moreover, lawsone‐loaded nanoparticle upregulated BAX gene expression, while downregulated BCL2expression, suggesting the activation of apoptotic pathway. The observed cytotoxic/apoptotic properties suggest that Lawson‐loaded PLGA‐FA‐CS‐NPs hold a great potential in pancreatic cancer treatment. |
| format | Article |
| id | doaj-art-61b68258c6aa428182b81f5760ca3ea1 |
| institution | Kabale University |
| issn | 1751-8741 1751-875X |
| language | English |
| publishDate | 2023-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | IET Nanobiotechnology |
| spelling | doaj-art-61b68258c6aa428182b81f5760ca3ea12025-02-03T06:45:05ZengWileyIET Nanobiotechnology1751-87411751-875X2023-07-0117542543710.1049/nbt2.12139Lawsone encapsulated polylactic‐co‐glycolic acid nanoparticles modified with chitosan‐folic acid successfully inhibited cell growth and triggered apoptosis in Panc‐1 cancer cellsHelia Ghafaripour0Masoud Homayouni Tabrizi1Ehsan Karimi2Niloofar Barati Naeeni3Department of Biology Mashhad Branch Islamic Azad University Mashhad IranDepartment of Biology Mashhad Branch Islamic Azad University Mashhad IranDepartment of Biology Mashhad Branch Islamic Azad University Mashhad IranDepartment of Biology Mashhad Branch Islamic Azad University Mashhad IranAbstract The present research aims to encapsulate lawsone in polylactic‐co‐glycolic acid (PLGA) nanoparticles modified with folic acid (FA) and chitosan (CS) to study its anticancer effects against Panc‐1 cells. The nanoparticles were analysed in means of shape/size and zeta potential index using scanning electron microscope and dynamic light scattering. High‐performance liquid chromatography was applied to evaluate the lawsone entrapment efficacy. The authors performed acridine orange/propidium iodide staining and flow cytometry to monitor apoptosis induction and cell cycle arrest. The expressions of apoptosis‐related genes (BAX and BCL‐2) were assessed by real time PCR. Nanoparticle antioxidative and antibacterial activities were examined by DPPH/ABTS scavenging assay, disk diffusion method, and minimum inhibitory concentration and minimum bactericidal concentration evaluation. The NPs were 229.65 nm, the encapsulation efficiency was 81%. The concentration of lawsone that exerts 50% cell growth inhibition (IC50) against Panc‐1 cells was calculated 118.4 μL. Apoptosis induction was evidenced by the increased number of orange cells and increased proportion of cells in G1‐Sub phase respectively. Moreover, lawsone‐loaded nanoparticle upregulated BAX gene expression, while downregulated BCL2expression, suggesting the activation of apoptotic pathway. The observed cytotoxic/apoptotic properties suggest that Lawson‐loaded PLGA‐FA‐CS‐NPs hold a great potential in pancreatic cancer treatment.https://doi.org/10.1049/nbt2.12139drug delivery systemsnanobiotechnologynanomedicinenanotechnologypolymer structure |
| spellingShingle | Helia Ghafaripour Masoud Homayouni Tabrizi Ehsan Karimi Niloofar Barati Naeeni Lawsone encapsulated polylactic‐co‐glycolic acid nanoparticles modified with chitosan‐folic acid successfully inhibited cell growth and triggered apoptosis in Panc‐1 cancer cells IET Nanobiotechnology drug delivery systems nanobiotechnology nanomedicine nanotechnology polymer structure |
| title | Lawsone encapsulated polylactic‐co‐glycolic acid nanoparticles modified with chitosan‐folic acid successfully inhibited cell growth and triggered apoptosis in Panc‐1 cancer cells |
| title_full | Lawsone encapsulated polylactic‐co‐glycolic acid nanoparticles modified with chitosan‐folic acid successfully inhibited cell growth and triggered apoptosis in Panc‐1 cancer cells |
| title_fullStr | Lawsone encapsulated polylactic‐co‐glycolic acid nanoparticles modified with chitosan‐folic acid successfully inhibited cell growth and triggered apoptosis in Panc‐1 cancer cells |
| title_full_unstemmed | Lawsone encapsulated polylactic‐co‐glycolic acid nanoparticles modified with chitosan‐folic acid successfully inhibited cell growth and triggered apoptosis in Panc‐1 cancer cells |
| title_short | Lawsone encapsulated polylactic‐co‐glycolic acid nanoparticles modified with chitosan‐folic acid successfully inhibited cell growth and triggered apoptosis in Panc‐1 cancer cells |
| title_sort | lawsone encapsulated polylactic co glycolic acid nanoparticles modified with chitosan folic acid successfully inhibited cell growth and triggered apoptosis in panc 1 cancer cells |
| topic | drug delivery systems nanobiotechnology nanomedicine nanotechnology polymer structure |
| url | https://doi.org/10.1049/nbt2.12139 |
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