Pharmacokinetic Analysis of Diosgenin in Rat Plasma by a UPLC-MS/MS Approach
Diosgenin, a steroidal sapogenin, has attracted attention worldwide owing to its pharmacological properties, including antitumor, cardiovascular protective, hypolipidemic, and anti-inflammatory effects. The current diosgenin analysis methods have the disadvantages of long analysis time and low sensi...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2022/5607347 |
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| author | Pei Liu Lin Xu Jing-han Guo Jin-hua Chang Xi-gang Liu He-fei Xue Ru-xing Wang Zhong-si Li Guang-xin Miao Cui-zhe Liu Jian-yu Zhou |
| author_facet | Pei Liu Lin Xu Jing-han Guo Jin-hua Chang Xi-gang Liu He-fei Xue Ru-xing Wang Zhong-si Li Guang-xin Miao Cui-zhe Liu Jian-yu Zhou |
| author_sort | Pei Liu |
| collection | DOAJ |
| description | Diosgenin, a steroidal sapogenin, has attracted attention worldwide owing to its pharmacological properties, including antitumor, cardiovascular protective, hypolipidemic, and anti-inflammatory effects. The current diosgenin analysis methods have the disadvantages of long analysis time and low sensitivity. The aim of the present study was to establish an efficient, sensitive ultrahigh-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) approach for pharmacokinetic analysis of diosgenin amorphous solid dispersion (ASD) using tanshinone IIA as an internal standard (IS). Male Sprague-Dawley rats were orally administered diosgenin ASD, and orbital blood samples were collected for analysis. Protein precipitation was performed with methanol-acetonitrile (50 : 50, v/v), and the analytes were separated under isocratic elution by applying acetonitrile and 0.03% formic acid aqueous solution at a ratio of 80 : 20 as the mobile phase. MS with positive electron spray ionization in multiple reaction monitoring modes was applied to determine diosgenin and IS with m/z 415.2⟶271.2 and m/z 295.2⟶277.1, respectively. This approach showed a low limit of quantification of 0.5 ng/ml for diosgenin and could detect this molecule at a concentration range of 0.5 to 1,500 ng/ml (r = 0.99725). The approach was found to have intra- and inter-day precision values ranging from 1.42% to 6.91% and from 1.25% to 3.68%, respectively. Additionally, the method showed an accuracy of -6.54 to 4.71%. The recoveries of diosgenin and tanshinone IIA were 85.81–100.27% and 98.29%, respectively, with negligible matrix effects. Diosgenin and IS were stable under multiple storage conditions. Pharmacokinetic analysis showed that the Cmax and AUC0⟶t of diosgenin ASD were significantly higher than those of the bulk drug. A sensitive, simple, UPLC-MS/MS analysis approach was established and used for the pharmacokinetic analysis of diosgenin ASD in rats after oral administration. |
| format | Article |
| id | doaj-art-61acfc08632a4537b8fd75009156ef40 |
| institution | Kabale University |
| issn | 2090-8873 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-61acfc08632a4537b8fd75009156ef402025-02-03T06:13:01ZengWileyJournal of Analytical Methods in Chemistry2090-88732022-01-01202210.1155/2022/5607347Pharmacokinetic Analysis of Diosgenin in Rat Plasma by a UPLC-MS/MS ApproachPei Liu0Lin Xu1Jing-han Guo2Jin-hua Chang3Xi-gang Liu4He-fei Xue5Ru-xing Wang6Zhong-si Li7Guang-xin Miao8Cui-zhe Liu9Jian-yu Zhou10Hebei Province Key Laboratory of Nerve Injury and RepairHebei Province Key Laboratory of Nerve Injury and RepairBeijing North Institute of BiotechnologyHebei Province Key Laboratory of Nerve Injury and RepairHebei Province Key Laboratory of Nerve Injury and RepairHebei Province Key Laboratory of Nerve Injury and RepairHebei Province Key Laboratory of Nerve Injury and RepairHebei Province Key Laboratory of Nerve Injury and RepairHebei Province Key Laboratory of Nerve Injury and RepairHebei Province Key Laboratory of Nerve Injury and RepairHebei Province Key Laboratory of Nerve Injury and RepairDiosgenin, a steroidal sapogenin, has attracted attention worldwide owing to its pharmacological properties, including antitumor, cardiovascular protective, hypolipidemic, and anti-inflammatory effects. The current diosgenin analysis methods have the disadvantages of long analysis time and low sensitivity. The aim of the present study was to establish an efficient, sensitive ultrahigh-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) approach for pharmacokinetic analysis of diosgenin amorphous solid dispersion (ASD) using tanshinone IIA as an internal standard (IS). Male Sprague-Dawley rats were orally administered diosgenin ASD, and orbital blood samples were collected for analysis. Protein precipitation was performed with methanol-acetonitrile (50 : 50, v/v), and the analytes were separated under isocratic elution by applying acetonitrile and 0.03% formic acid aqueous solution at a ratio of 80 : 20 as the mobile phase. MS with positive electron spray ionization in multiple reaction monitoring modes was applied to determine diosgenin and IS with m/z 415.2⟶271.2 and m/z 295.2⟶277.1, respectively. This approach showed a low limit of quantification of 0.5 ng/ml for diosgenin and could detect this molecule at a concentration range of 0.5 to 1,500 ng/ml (r = 0.99725). The approach was found to have intra- and inter-day precision values ranging from 1.42% to 6.91% and from 1.25% to 3.68%, respectively. Additionally, the method showed an accuracy of -6.54 to 4.71%. The recoveries of diosgenin and tanshinone IIA were 85.81–100.27% and 98.29%, respectively, with negligible matrix effects. Diosgenin and IS were stable under multiple storage conditions. Pharmacokinetic analysis showed that the Cmax and AUC0⟶t of diosgenin ASD were significantly higher than those of the bulk drug. A sensitive, simple, UPLC-MS/MS analysis approach was established and used for the pharmacokinetic analysis of diosgenin ASD in rats after oral administration.http://dx.doi.org/10.1155/2022/5607347 |
| spellingShingle | Pei Liu Lin Xu Jing-han Guo Jin-hua Chang Xi-gang Liu He-fei Xue Ru-xing Wang Zhong-si Li Guang-xin Miao Cui-zhe Liu Jian-yu Zhou Pharmacokinetic Analysis of Diosgenin in Rat Plasma by a UPLC-MS/MS Approach Journal of Analytical Methods in Chemistry |
| title | Pharmacokinetic Analysis of Diosgenin in Rat Plasma by a UPLC-MS/MS Approach |
| title_full | Pharmacokinetic Analysis of Diosgenin in Rat Plasma by a UPLC-MS/MS Approach |
| title_fullStr | Pharmacokinetic Analysis of Diosgenin in Rat Plasma by a UPLC-MS/MS Approach |
| title_full_unstemmed | Pharmacokinetic Analysis of Diosgenin in Rat Plasma by a UPLC-MS/MS Approach |
| title_short | Pharmacokinetic Analysis of Diosgenin in Rat Plasma by a UPLC-MS/MS Approach |
| title_sort | pharmacokinetic analysis of diosgenin in rat plasma by a uplc ms ms approach |
| url | http://dx.doi.org/10.1155/2022/5607347 |
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