AGE/RAGE-Induced EMP Release via the NOX-Derived ROS Pathway
Objective. Diabetes is associated with accelerated formation of advanced glycation end products (AGEs) that are extensively found in circulating endothelial microparticles (EMPs). This study aimed to investigate whether AGEs have a direct effect on EMP formation and the possible underlying mechanism...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2018/6823058 |
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| _version_ | 1832549304489738240 |
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| author | Ying-Hua Chen Zhang-Wei Chen Hong-Mei Li Xin-Feng Yan Bo Feng |
| author_facet | Ying-Hua Chen Zhang-Wei Chen Hong-Mei Li Xin-Feng Yan Bo Feng |
| author_sort | Ying-Hua Chen |
| collection | DOAJ |
| description | Objective. Diabetes is associated with accelerated formation of advanced glycation end products (AGEs) that are extensively found in circulating endothelial microparticles (EMPs). This study aimed to investigate whether AGEs have a direct effect on EMP formation and the possible underlying mechanism. Methods. In vitro, cultured human umbilical vein endothelial cells (HUVECs) were incubated with AGEs (200 and 400 μg/ml) for 24 hours with or without pretreatment with anti-RAGE antibody, NOX inhibitor, or ROS scavenger. The number of CD31-positive EMPs was assessed by flow cytometry. Results. The number of EMPs was significantly increased in HUVECs stimulated by AGEs in a dose-dependent manner. In addition, receptors for AGEs (RAGE), NAD(P)H oxidase (NOX), and reactive oxygen species (ROS) were increased by AGEs as compared to the control group. These changes could be reversed when HUVECs were pretreated with anti-RAGE antibody. Moreover, inhibition of NOX as well as antioxidant treatment reduced the release of EMPs induced by AGEs. Conclusion. Our study suggested that AGEs increased EMP generation, which was mediated by RAGE signaling through NOX-derived ROS. |
| format | Article |
| id | doaj-art-61936d7b862b49279b459dc4125f9fd3 |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-61936d7b862b49279b459dc4125f9fd32025-02-03T06:11:39ZengWileyJournal of Diabetes Research2314-67452314-67532018-01-01201810.1155/2018/68230586823058AGE/RAGE-Induced EMP Release via the NOX-Derived ROS PathwayYing-Hua Chen0Zhang-Wei Chen1Hong-Mei Li2Xin-Feng Yan3Bo Feng4Department of Endocrinology, East Hospital, Tongji University, Shanghai 200120, ChinaDepartment of Cardiology, Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, ChinaDepartment of Endocrinology, East Hospital, Tongji University, Shanghai 200120, ChinaDepartment of Endocrinology, East Hospital, Tongji University, Shanghai 200120, ChinaDepartment of Endocrinology, East Hospital, Tongji University, Shanghai 200120, ChinaObjective. Diabetes is associated with accelerated formation of advanced glycation end products (AGEs) that are extensively found in circulating endothelial microparticles (EMPs). This study aimed to investigate whether AGEs have a direct effect on EMP formation and the possible underlying mechanism. Methods. In vitro, cultured human umbilical vein endothelial cells (HUVECs) were incubated with AGEs (200 and 400 μg/ml) for 24 hours with or without pretreatment with anti-RAGE antibody, NOX inhibitor, or ROS scavenger. The number of CD31-positive EMPs was assessed by flow cytometry. Results. The number of EMPs was significantly increased in HUVECs stimulated by AGEs in a dose-dependent manner. In addition, receptors for AGEs (RAGE), NAD(P)H oxidase (NOX), and reactive oxygen species (ROS) were increased by AGEs as compared to the control group. These changes could be reversed when HUVECs were pretreated with anti-RAGE antibody. Moreover, inhibition of NOX as well as antioxidant treatment reduced the release of EMPs induced by AGEs. Conclusion. Our study suggested that AGEs increased EMP generation, which was mediated by RAGE signaling through NOX-derived ROS.http://dx.doi.org/10.1155/2018/6823058 |
| spellingShingle | Ying-Hua Chen Zhang-Wei Chen Hong-Mei Li Xin-Feng Yan Bo Feng AGE/RAGE-Induced EMP Release via the NOX-Derived ROS Pathway Journal of Diabetes Research |
| title | AGE/RAGE-Induced EMP Release via the NOX-Derived ROS Pathway |
| title_full | AGE/RAGE-Induced EMP Release via the NOX-Derived ROS Pathway |
| title_fullStr | AGE/RAGE-Induced EMP Release via the NOX-Derived ROS Pathway |
| title_full_unstemmed | AGE/RAGE-Induced EMP Release via the NOX-Derived ROS Pathway |
| title_short | AGE/RAGE-Induced EMP Release via the NOX-Derived ROS Pathway |
| title_sort | age rage induced emp release via the nox derived ros pathway |
| url | http://dx.doi.org/10.1155/2018/6823058 |
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