Aqp 9 and Brain Tumour Stem Cells
Several studies have implicated the aquaporins (aqp) 1, 4, and 9 in the pathogenesis of malignant brain tumours, suggesting that they contribute to motility, invasiveness, and oedema formation and facilitate metabolism in tumour cells under hypoxic conditions. We have studied the expression of aqp1,...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/915176 |
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| author | Guri Fossdal Einar O. Vik-Mo Cecilie Sandberg Mercy Varghese Mari Kaarbø Emily Telmo Iver A. Langmoen Wayne Murrell |
| author_facet | Guri Fossdal Einar O. Vik-Mo Cecilie Sandberg Mercy Varghese Mari Kaarbø Emily Telmo Iver A. Langmoen Wayne Murrell |
| author_sort | Guri Fossdal |
| collection | DOAJ |
| description | Several studies have implicated the aquaporins (aqp) 1, 4, and 9 in the pathogenesis of malignant brain tumours, suggesting that they contribute to motility, invasiveness, and oedema formation and facilitate metabolism in tumour cells under hypoxic conditions. We have studied the expression of aqp1, 4, and 9 in biopsies from glioblastomas, isolated tumour stem cells grown in a tumoursphere assay and analyzed the progenitor and differentiated cells from these cultures. We have compared these to the situation in normal rat brain, its stem cells, and differentiated cells derived thereof. In short, qPCR in tumour tissue showed presence of aqp1, 4, and 9. In the tumour progenitor population, aqp9 was markedly more highly expressed, whilst in tumour-derived differentiated cells, aqp4 was downregulated. However, immunostaining did not reveal increased protein expression of aqp9 in the tumourspheres containing progenitor cells; in contrast, its expression (both mRNA and protein) was high in differentiated cultures. We, therefore, propose that aquaporin 9 may have a central role in the tumorigenesis of glioblastoma. |
| format | Article |
| id | doaj-art-617895b143784d7a80ae30952bfbd5ec |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-617895b143784d7a80ae30952bfbd5ec2025-02-03T06:13:36ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/915176915176Aqp 9 and Brain Tumour Stem CellsGuri Fossdal0Einar O. Vik-Mo1Cecilie Sandberg2Mercy Varghese3Mari Kaarbø4Emily Telmo5Iver A. Langmoen6Wayne Murrell7Vilhelm Magnus laboratory, Institute for Surgical Research, Norwegian National Hospital, Oslo University Hospital, 0450 Oslo, NorwayVilhelm Magnus laboratory, Institute for Surgical Research, Norwegian National Hospital, Oslo University Hospital, 0450 Oslo, NorwayVilhelm Magnus laboratory, Institute for Surgical Research, Norwegian National Hospital, Oslo University Hospital, 0450 Oslo, NorwayVilhelm Magnus laboratory, Institute for Surgical Research, Norwegian National Hospital, Oslo University Hospital, 0450 Oslo, NorwayVilhelm Magnus laboratory, Institute for Surgical Research, Norwegian National Hospital, Oslo University Hospital, 0450 Oslo, NorwayVilhelm Magnus laboratory, Institute for Surgical Research, Norwegian National Hospital, Oslo University Hospital, 0450 Oslo, NorwayVilhelm Magnus laboratory, Institute for Surgical Research, Norwegian National Hospital, Oslo University Hospital, 0450 Oslo, NorwayVilhelm Magnus laboratory, Institute for Surgical Research, Norwegian National Hospital, Oslo University Hospital, 0450 Oslo, NorwaySeveral studies have implicated the aquaporins (aqp) 1, 4, and 9 in the pathogenesis of malignant brain tumours, suggesting that they contribute to motility, invasiveness, and oedema formation and facilitate metabolism in tumour cells under hypoxic conditions. We have studied the expression of aqp1, 4, and 9 in biopsies from glioblastomas, isolated tumour stem cells grown in a tumoursphere assay and analyzed the progenitor and differentiated cells from these cultures. We have compared these to the situation in normal rat brain, its stem cells, and differentiated cells derived thereof. In short, qPCR in tumour tissue showed presence of aqp1, 4, and 9. In the tumour progenitor population, aqp9 was markedly more highly expressed, whilst in tumour-derived differentiated cells, aqp4 was downregulated. However, immunostaining did not reveal increased protein expression of aqp9 in the tumourspheres containing progenitor cells; in contrast, its expression (both mRNA and protein) was high in differentiated cultures. We, therefore, propose that aquaporin 9 may have a central role in the tumorigenesis of glioblastoma.http://dx.doi.org/10.1100/2012/915176 |
| spellingShingle | Guri Fossdal Einar O. Vik-Mo Cecilie Sandberg Mercy Varghese Mari Kaarbø Emily Telmo Iver A. Langmoen Wayne Murrell Aqp 9 and Brain Tumour Stem Cells The Scientific World Journal |
| title | Aqp 9 and Brain Tumour Stem Cells |
| title_full | Aqp 9 and Brain Tumour Stem Cells |
| title_fullStr | Aqp 9 and Brain Tumour Stem Cells |
| title_full_unstemmed | Aqp 9 and Brain Tumour Stem Cells |
| title_short | Aqp 9 and Brain Tumour Stem Cells |
| title_sort | aqp 9 and brain tumour stem cells |
| url | http://dx.doi.org/10.1100/2012/915176 |
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