First Detection of <i>Alphacoronavirus</i> in Bats from the World’s Largest Wetland, the Pantanal, Brazil
Coronaviruses (CoV) infect a wide variety of hosts, causing epidemics in humans, birds, and mammals over the years. Bats (order Chiroptera) are one of the natural hosts of the Coronaviridae family. They represent 40% of the total number of mammal species in the Pantanal, a biodiversity hotspot in So...
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| author | Tayane B. S. Magalhães Amanda de O. Viana Thiago B. F. Semedo Juliane S. Saldanha Nicole A. dos Reis Nathalia de A. Pereira Rachel V. P. de Barros Hannah R. Miranda Gabriella C. Almeida Desyrée Y. S. R. Ozaki Giovana S. Caleiro Gustavo O. Fenner Fernanda P. Vizu Theo Kraiser Thais P. Carvalho Luciano M. Thomazelli Erick G. Dorlass Clarice W. Arns Helena L. Ferreira Erika Hingst-Zaher Rogério Vieira Rossi Guilherme S. T. Garbino Edison L. Durigon Jansen de Araujo Daniel M. de Aguiar |
| author_facet | Tayane B. S. Magalhães Amanda de O. Viana Thiago B. F. Semedo Juliane S. Saldanha Nicole A. dos Reis Nathalia de A. Pereira Rachel V. P. de Barros Hannah R. Miranda Gabriella C. Almeida Desyrée Y. S. R. Ozaki Giovana S. Caleiro Gustavo O. Fenner Fernanda P. Vizu Theo Kraiser Thais P. Carvalho Luciano M. Thomazelli Erick G. Dorlass Clarice W. Arns Helena L. Ferreira Erika Hingst-Zaher Rogério Vieira Rossi Guilherme S. T. Garbino Edison L. Durigon Jansen de Araujo Daniel M. de Aguiar |
| author_sort | Tayane B. S. Magalhães |
| collection | DOAJ |
| description | Coronaviruses (CoV) infect a wide variety of hosts, causing epidemics in humans, birds, and mammals over the years. Bats (order Chiroptera) are one of the natural hosts of the Coronaviridae family. They represent 40% of the total number of mammal species in the Pantanal, a biodiversity hotspot in South America. Given the recent SARS-CoV-2 pandemic, we investigated the presence of CoV in bats captured in the Brazilian Pantanal. Oral and rectal swabs collected in 2021 from 419 bats were analyzed using Pancoronavirus-nested PCR targeting the RNA-dependent RNA-polymerase (RdRp) gene. Orthocoronavirinae was detected in 16.7% (70/419) of the bats; nine samples were sequenced, confirming that <i>Carollia perspicillata</i> (4), <i>Phyllostomus hastatus</i> (2), <i>Desmodus rotundus</i> (1), <i>Molossus rufus</i> (1), and <i>Myotis</i> cf. <i>nigricans</i> (1) collected in buildings formally used by humans were infected by <i>Alphacoronavirus</i> genera. This is the first description of <i>Alphacoronavirus</i> in bats from the Pantanal. As they are natural reservoirs of CoVs, constant monitoring of bats is important to comprehend the epidemiology of emerging viruses, especially in the Pantanal biome. |
| format | Article |
| id | doaj-art-6172054cd84b45a6a281bc15dbfa60fe |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-6172054cd84b45a6a281bc15dbfa60fe2025-01-24T13:44:45ZengMDPI AGPathogens2076-08172025-01-011415810.3390/pathogens14010058First Detection of <i>Alphacoronavirus</i> in Bats from the World’s Largest Wetland, the Pantanal, BrazilTayane B. S. Magalhães0Amanda de O. Viana1Thiago B. F. Semedo2Juliane S. Saldanha3Nicole A. dos Reis4Nathalia de A. Pereira5Rachel V. P. de Barros6Hannah R. Miranda7Gabriella C. Almeida8Desyrée Y. S. R. Ozaki9Giovana S. Caleiro10Gustavo O. Fenner11Fernanda P. Vizu12Theo Kraiser13Thais P. Carvalho14Luciano M. Thomazelli15Erick G. Dorlass16Clarice W. Arns17Helena L. Ferreira18Erika Hingst-Zaher19Rogério Vieira Rossi20Guilherme S. T. Garbino21Edison L. Durigon22Jansen de Araujo23Daniel M. de Aguiar24Laboratório de Virologia e Rickettsioses, Faculdade de Medicina Veterinária, Universidade Federal de Mato Grosso, Fernando Correa da Costa, 2367, Cuiabá 78060-900, BrazilLaboratório de Virologia Clínica e Molecular, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo 05508-000, BrazilLaboratório de Virologia e Rickettsioses, Faculdade de Medicina Veterinária, Universidade Federal de Mato Grosso, Fernando Correa da Costa, 2367, Cuiabá 78060-900, BrazilLaboratório de Virologia e Rickettsioses, Faculdade de Medicina Veterinária, Universidade Federal de Mato Grosso, Fernando Correa da Costa, 2367, Cuiabá 78060-900, BrazilLaboratório de Pesquisa em Vírus Emergentes, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo 05508-000, BrazilLaboratório de Virologia e Rickettsioses, Faculdade de Medicina Veterinária, Universidade Federal de Mato Grosso, Fernando Correa da Costa, 2367, Cuiabá 78060-900, BrazilLaboratório de Virologia e Rickettsioses, Faculdade de Medicina Veterinária, Universidade Federal de Mato Grosso, Fernando Correa da Costa, 2367, Cuiabá 78060-900, BrazilLaboratório de Virologia e Rickettsioses, Faculdade de Medicina Veterinária, Universidade Federal de Mato Grosso, Fernando Correa da Costa, 2367, Cuiabá 78060-900, BrazilLaboratório de Virologia e Rickettsioses, Faculdade de Medicina Veterinária, Universidade Federal de Mato Grosso, Fernando Correa da Costa, 2367, Cuiabá 78060-900, BrazilLaboratório de Pesquisa em Vírus Emergentes, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo 05508-000, BrazilLaboratório de Pesquisa em Vírus Emergentes, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo 05508-000, BrazilLaboratório de Pesquisa em Vírus Emergentes, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo 05508-000, BrazilLaboratório de Pesquisa em Vírus Emergentes, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo 05508-000, BrazilLaboratório de Pesquisa em Vírus Emergentes, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo 05508-000, BrazilLaboratório de Pesquisa em Vírus Emergentes, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo 05508-000, BrazilLaboratório de Virologia Clínica e Molecular, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo 05508-000, BrazilLaboratório de Virologia Clínica e Molecular, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo 05508-000, BrazilDepartamento de Genética, Evolução, Microbiologia e Imunologia, Instituto de Biociências, Universidade Estadual de Campinas, Campinas 13083-862, BrazilFaculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga 13635-900, BrazilMuseu Biológico, Instituto Butantan, São Paulo 05585-000, BrazilLaboratorio de Mastozoologia, Instituto de Biociencias, Universidade Federal de Mato Grosso, Cuiabá 78060-900, BrazilMuseu de Zoologia João Moojen, Departamento de Biologia Animal, Universidade Federal de Viçosa, Viçosa 36570-900, BrazilLaboratório de Virologia Clínica e Molecular, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo 05508-000, BrazilLaboratório de Pesquisa em Vírus Emergentes, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo 05508-000, BrazilLaboratório de Virologia e Rickettsioses, Faculdade de Medicina Veterinária, Universidade Federal de Mato Grosso, Fernando Correa da Costa, 2367, Cuiabá 78060-900, BrazilCoronaviruses (CoV) infect a wide variety of hosts, causing epidemics in humans, birds, and mammals over the years. Bats (order Chiroptera) are one of the natural hosts of the Coronaviridae family. They represent 40% of the total number of mammal species in the Pantanal, a biodiversity hotspot in South America. Given the recent SARS-CoV-2 pandemic, we investigated the presence of CoV in bats captured in the Brazilian Pantanal. Oral and rectal swabs collected in 2021 from 419 bats were analyzed using Pancoronavirus-nested PCR targeting the RNA-dependent RNA-polymerase (RdRp) gene. Orthocoronavirinae was detected in 16.7% (70/419) of the bats; nine samples were sequenced, confirming that <i>Carollia perspicillata</i> (4), <i>Phyllostomus hastatus</i> (2), <i>Desmodus rotundus</i> (1), <i>Molossus rufus</i> (1), and <i>Myotis</i> cf. <i>nigricans</i> (1) collected in buildings formally used by humans were infected by <i>Alphacoronavirus</i> genera. This is the first description of <i>Alphacoronavirus</i> in bats from the Pantanal. As they are natural reservoirs of CoVs, constant monitoring of bats is important to comprehend the epidemiology of emerging viruses, especially in the Pantanal biome.https://www.mdpi.com/2076-0817/14/1/58OrthocoronavirusChiropteramolecular detectionPancoronavirus RT-PCRwestern Brazil |
| spellingShingle | Tayane B. S. Magalhães Amanda de O. Viana Thiago B. F. Semedo Juliane S. Saldanha Nicole A. dos Reis Nathalia de A. Pereira Rachel V. P. de Barros Hannah R. Miranda Gabriella C. Almeida Desyrée Y. S. R. Ozaki Giovana S. Caleiro Gustavo O. Fenner Fernanda P. Vizu Theo Kraiser Thais P. Carvalho Luciano M. Thomazelli Erick G. Dorlass Clarice W. Arns Helena L. Ferreira Erika Hingst-Zaher Rogério Vieira Rossi Guilherme S. T. Garbino Edison L. Durigon Jansen de Araujo Daniel M. de Aguiar First Detection of <i>Alphacoronavirus</i> in Bats from the World’s Largest Wetland, the Pantanal, Brazil Pathogens Orthocoronavirus Chiroptera molecular detection Pancoronavirus RT-PCR western Brazil |
| title | First Detection of <i>Alphacoronavirus</i> in Bats from the World’s Largest Wetland, the Pantanal, Brazil |
| title_full | First Detection of <i>Alphacoronavirus</i> in Bats from the World’s Largest Wetland, the Pantanal, Brazil |
| title_fullStr | First Detection of <i>Alphacoronavirus</i> in Bats from the World’s Largest Wetland, the Pantanal, Brazil |
| title_full_unstemmed | First Detection of <i>Alphacoronavirus</i> in Bats from the World’s Largest Wetland, the Pantanal, Brazil |
| title_short | First Detection of <i>Alphacoronavirus</i> in Bats from the World’s Largest Wetland, the Pantanal, Brazil |
| title_sort | first detection of i alphacoronavirus i in bats from the world s largest wetland the pantanal brazil |
| topic | Orthocoronavirus Chiroptera molecular detection Pancoronavirus RT-PCR western Brazil |
| url | https://www.mdpi.com/2076-0817/14/1/58 |
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