Dietary Erucic Acid Induces Fat Accumulation, Hepatic Oxidative Damage, and Abnormal Lipid Metabolism in Nile Tilapia (Oreochromis niloticus)

Erucic acid (EA) in rapeseed oil has adverse effects on terrestrial animal and fish health. However, its antinutritional role in fish remains unclear due to the limited information on EA. Therefore, this study was conducted to assess the impact of EA on growth performance, antioxidative capacity, fa...

Full description

Saved in:
Bibliographic Details
Main Authors: Dingfei Ma, Qiangwei Li, Yuanyuan Xie, Youqin Kong, Zhili Ding, Jinyun Ye, Chenglong Wu, Yan Liu
Format: Article
Language:English
Published: Wiley 2024-01-01
Series:Aquaculture Nutrition
Online Access:http://dx.doi.org/10.1155/2024/6670740
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850120432264413184
author Dingfei Ma
Qiangwei Li
Yuanyuan Xie
Youqin Kong
Zhili Ding
Jinyun Ye
Chenglong Wu
Yan Liu
author_facet Dingfei Ma
Qiangwei Li
Yuanyuan Xie
Youqin Kong
Zhili Ding
Jinyun Ye
Chenglong Wu
Yan Liu
author_sort Dingfei Ma
collection DOAJ
description Erucic acid (EA) in rapeseed oil has adverse effects on terrestrial animal and fish health. However, its antinutritional role in fish remains unclear due to the limited information on EA. Therefore, this study was conducted to assess the impact of EA on growth performance, antioxidative capacity, fatty acid profile, and lipid metabolism in tilapia. Six diets containing different amounts of EA (0, 3, 6, 12, 20, and 27 g/kg diet) were fed to tilapia (initial weight: 3.01 ± 0.01 g) for 8 weeks. The results exhibited that dietary EA did not affect growth performance but remarkedly increased the crude lipid contents (in the whole body, liver, and muscle). It also markedly increased the levels of low-density lipoprotein cholesterol, total cholesterol, nonesterified fatty acids, and triglyceride in the liver and serum in a dose-dependent manner. The EA groups had lower values of total superoxide dismutase, total antioxidant capacity, catalase, and higher activities of aspartate aminotransferase and alanine aminotransferase, as dietary EA levels increased. Feeding fish with diets containing EA (20 and 27 g/kg diet) significantly increased the malondialdehyde content. Moreover, dietary EA greatly altered the fatty acid profile in the liver and muscle. It especially elevated the percentages of C18 : 2n-6, C20 : 1n-9, and C22 : 1n-9 while decreasing the C18 : 0 and C16 : 0 levels. When the levels of EA in diets were 12, 20, and 27 g/kg, genes correlated with lipophagy, lipolysis, and β-oxidation were significantly reduced. Meanwhile, genes concerned in triglyceride synthesis were largely increased in the liver and muscle. In summary, high-dose EA (20 g/kg diet) in the diets significantly induced fat accumulation, hepatic oxidative damage, and abnormal lipid metabolism in tilapia. The current findings expand our understanding on the antinutritional role of EA in lipid homeostasis and fish health.
format Article
id doaj-art-6152c4b3c80e412fbfdc8b80175cae41
institution OA Journals
issn 1365-2095
language English
publishDate 2024-01-01
publisher Wiley
record_format Article
series Aquaculture Nutrition
spelling doaj-art-6152c4b3c80e412fbfdc8b80175cae412025-08-20T02:35:21ZengWileyAquaculture Nutrition1365-20952024-01-01202410.1155/2024/6670740Dietary Erucic Acid Induces Fat Accumulation, Hepatic Oxidative Damage, and Abnormal Lipid Metabolism in Nile Tilapia (Oreochromis niloticus)Dingfei Ma0Qiangwei Li1Yuanyuan Xie2Youqin Kong3Zhili Ding4Jinyun Ye5Chenglong Wu6Yan Liu7National-Local Joint Engineering Laboratory of Aquatic Animal Genetic Breeding and Nutrition (Zhejiang)National-Local Joint Engineering Laboratory of Aquatic Animal Genetic Breeding and Nutrition (Zhejiang)National-Local Joint Engineering Laboratory of Aquatic Animal Genetic Breeding and Nutrition (Zhejiang)National-Local Joint Engineering Laboratory of Aquatic Animal Genetic Breeding and Nutrition (Zhejiang)National-Local Joint Engineering Laboratory of Aquatic Animal Genetic Breeding and Nutrition (Zhejiang)National-Local Joint Engineering Laboratory of Aquatic Animal Genetic Breeding and Nutrition (Zhejiang)National-Local Joint Engineering Laboratory of Aquatic Animal Genetic Breeding and Nutrition (Zhejiang)National-Local Joint Engineering Laboratory of Aquatic Animal Genetic Breeding and Nutrition (Zhejiang)Erucic acid (EA) in rapeseed oil has adverse effects on terrestrial animal and fish health. However, its antinutritional role in fish remains unclear due to the limited information on EA. Therefore, this study was conducted to assess the impact of EA on growth performance, antioxidative capacity, fatty acid profile, and lipid metabolism in tilapia. Six diets containing different amounts of EA (0, 3, 6, 12, 20, and 27 g/kg diet) were fed to tilapia (initial weight: 3.01 ± 0.01 g) for 8 weeks. The results exhibited that dietary EA did not affect growth performance but remarkedly increased the crude lipid contents (in the whole body, liver, and muscle). It also markedly increased the levels of low-density lipoprotein cholesterol, total cholesterol, nonesterified fatty acids, and triglyceride in the liver and serum in a dose-dependent manner. The EA groups had lower values of total superoxide dismutase, total antioxidant capacity, catalase, and higher activities of aspartate aminotransferase and alanine aminotransferase, as dietary EA levels increased. Feeding fish with diets containing EA (20 and 27 g/kg diet) significantly increased the malondialdehyde content. Moreover, dietary EA greatly altered the fatty acid profile in the liver and muscle. It especially elevated the percentages of C18 : 2n-6, C20 : 1n-9, and C22 : 1n-9 while decreasing the C18 : 0 and C16 : 0 levels. When the levels of EA in diets were 12, 20, and 27 g/kg, genes correlated with lipophagy, lipolysis, and β-oxidation were significantly reduced. Meanwhile, genes concerned in triglyceride synthesis were largely increased in the liver and muscle. In summary, high-dose EA (20 g/kg diet) in the diets significantly induced fat accumulation, hepatic oxidative damage, and abnormal lipid metabolism in tilapia. The current findings expand our understanding on the antinutritional role of EA in lipid homeostasis and fish health.http://dx.doi.org/10.1155/2024/6670740
spellingShingle Dingfei Ma
Qiangwei Li
Yuanyuan Xie
Youqin Kong
Zhili Ding
Jinyun Ye
Chenglong Wu
Yan Liu
Dietary Erucic Acid Induces Fat Accumulation, Hepatic Oxidative Damage, and Abnormal Lipid Metabolism in Nile Tilapia (Oreochromis niloticus)
Aquaculture Nutrition
title Dietary Erucic Acid Induces Fat Accumulation, Hepatic Oxidative Damage, and Abnormal Lipid Metabolism in Nile Tilapia (Oreochromis niloticus)
title_full Dietary Erucic Acid Induces Fat Accumulation, Hepatic Oxidative Damage, and Abnormal Lipid Metabolism in Nile Tilapia (Oreochromis niloticus)
title_fullStr Dietary Erucic Acid Induces Fat Accumulation, Hepatic Oxidative Damage, and Abnormal Lipid Metabolism in Nile Tilapia (Oreochromis niloticus)
title_full_unstemmed Dietary Erucic Acid Induces Fat Accumulation, Hepatic Oxidative Damage, and Abnormal Lipid Metabolism in Nile Tilapia (Oreochromis niloticus)
title_short Dietary Erucic Acid Induces Fat Accumulation, Hepatic Oxidative Damage, and Abnormal Lipid Metabolism in Nile Tilapia (Oreochromis niloticus)
title_sort dietary erucic acid induces fat accumulation hepatic oxidative damage and abnormal lipid metabolism in nile tilapia oreochromis niloticus
url http://dx.doi.org/10.1155/2024/6670740
work_keys_str_mv AT dingfeima dietaryerucicacidinducesfataccumulationhepaticoxidativedamageandabnormallipidmetabolisminniletilapiaoreochromisniloticus
AT qiangweili dietaryerucicacidinducesfataccumulationhepaticoxidativedamageandabnormallipidmetabolisminniletilapiaoreochromisniloticus
AT yuanyuanxie dietaryerucicacidinducesfataccumulationhepaticoxidativedamageandabnormallipidmetabolisminniletilapiaoreochromisniloticus
AT youqinkong dietaryerucicacidinducesfataccumulationhepaticoxidativedamageandabnormallipidmetabolisminniletilapiaoreochromisniloticus
AT zhiliding dietaryerucicacidinducesfataccumulationhepaticoxidativedamageandabnormallipidmetabolisminniletilapiaoreochromisniloticus
AT jinyunye dietaryerucicacidinducesfataccumulationhepaticoxidativedamageandabnormallipidmetabolisminniletilapiaoreochromisniloticus
AT chenglongwu dietaryerucicacidinducesfataccumulationhepaticoxidativedamageandabnormallipidmetabolisminniletilapiaoreochromisniloticus
AT yanliu dietaryerucicacidinducesfataccumulationhepaticoxidativedamageandabnormallipidmetabolisminniletilapiaoreochromisniloticus