Autoserum: An Optimal Supplement for Bone Marrow Mesenchymal Stem Cells of Liver-Injured Rats
Mesenchymal stem cells (MSCs) are an attractive source for the clinical cell therapy of liver injury. Although the use of adult serum, platelet lysate, or cord blood serum solves some of the problems caused by fetal bovine serum (FBS), the allogeneic immune response, contamination, and donor-to-dono...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2015/459580 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832556186481721344 |
|---|---|
| author | Qinglin Zhang Xun Sun Jianxun Ding Ping He Yujia Liu Hongjing Cheng Changlin Zhou Xiangwei Meng |
| author_facet | Qinglin Zhang Xun Sun Jianxun Ding Ping He Yujia Liu Hongjing Cheng Changlin Zhou Xiangwei Meng |
| author_sort | Qinglin Zhang |
| collection | DOAJ |
| description | Mesenchymal stem cells (MSCs) are an attractive source for the clinical cell therapy of liver injury. Although the use of adult serum, platelet lysate, or cord blood serum solves some of the problems caused by fetal bovine serum (FBS), the allogeneic immune response, contamination, and donor-to-donor and donor-to-receptor differences still obstruct the application of MSCs. In this study, the influences of autoserum from liver-injured rats (LIRs) and allogeneic serum from healthy rats on the isolation and culture of bone marrow MSCs (BMSCs) were examined and compared to FBS. The results showed that BMSCs cultured with autoserum or allogeneic serum exhibited better MSC-specific morphology, lower rate of cell senescent, and higher proliferation kinetics than those with FBS. In addition, autoserum promoted the osteogenic differentiation potential of BMSCs as allogeneic serum did. Although there were no significant differences in proliferation activity, immunophenotypic characterization, and differentiation potential between BMSCs cultured with autoserum and those with allogeneic serum, the potential adverse immunological reactions in patients with allogeneic material transplantation must be considered. We therefore believe that the autoserum from liver-injured patients may be a better choice for MSC expansion to meet the needs of liver injury therapy. |
| format | Article |
| id | doaj-art-6145a6fca1804cffa7efca872da44d03 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-6145a6fca1804cffa7efca872da44d032025-02-03T05:45:59ZengWileyStem Cells International1687-966X1687-96782015-01-01201510.1155/2015/459580459580Autoserum: An Optimal Supplement for Bone Marrow Mesenchymal Stem Cells of Liver-Injured RatsQinglin Zhang0Xun Sun1Jianxun Ding2Ping He3Yujia Liu4Hongjing Cheng5Changlin Zhou6Xiangwei Meng7Department of Gastroenterology, The First Hospital of Jilin University, Changchun 130021, ChinaDepartment of Pathology, The First Hospital of Jilin University, Changchun 130021, ChinaKey Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, ChinaDepartment of Gastroenterology, The First Hospital of Jilin University, Changchun 130021, ChinaDepartment of Endocrinology, The First Hospital of Jilin University, Changchun 130021, ChinaDepartment of Gastroenterology, The First Hospital of Jilin University, Changchun 130021, ChinaDepartment of Gastroenterology, The First Hospital of Jilin University, Changchun 130021, ChinaDepartment of Gastroenterology, The First Hospital of Jilin University, Changchun 130021, ChinaMesenchymal stem cells (MSCs) are an attractive source for the clinical cell therapy of liver injury. Although the use of adult serum, platelet lysate, or cord blood serum solves some of the problems caused by fetal bovine serum (FBS), the allogeneic immune response, contamination, and donor-to-donor and donor-to-receptor differences still obstruct the application of MSCs. In this study, the influences of autoserum from liver-injured rats (LIRs) and allogeneic serum from healthy rats on the isolation and culture of bone marrow MSCs (BMSCs) were examined and compared to FBS. The results showed that BMSCs cultured with autoserum or allogeneic serum exhibited better MSC-specific morphology, lower rate of cell senescent, and higher proliferation kinetics than those with FBS. In addition, autoserum promoted the osteogenic differentiation potential of BMSCs as allogeneic serum did. Although there were no significant differences in proliferation activity, immunophenotypic characterization, and differentiation potential between BMSCs cultured with autoserum and those with allogeneic serum, the potential adverse immunological reactions in patients with allogeneic material transplantation must be considered. We therefore believe that the autoserum from liver-injured patients may be a better choice for MSC expansion to meet the needs of liver injury therapy.http://dx.doi.org/10.1155/2015/459580 |
| spellingShingle | Qinglin Zhang Xun Sun Jianxun Ding Ping He Yujia Liu Hongjing Cheng Changlin Zhou Xiangwei Meng Autoserum: An Optimal Supplement for Bone Marrow Mesenchymal Stem Cells of Liver-Injured Rats Stem Cells International |
| title | Autoserum: An Optimal Supplement for Bone Marrow Mesenchymal Stem Cells of Liver-Injured Rats |
| title_full | Autoserum: An Optimal Supplement for Bone Marrow Mesenchymal Stem Cells of Liver-Injured Rats |
| title_fullStr | Autoserum: An Optimal Supplement for Bone Marrow Mesenchymal Stem Cells of Liver-Injured Rats |
| title_full_unstemmed | Autoserum: An Optimal Supplement for Bone Marrow Mesenchymal Stem Cells of Liver-Injured Rats |
| title_short | Autoserum: An Optimal Supplement for Bone Marrow Mesenchymal Stem Cells of Liver-Injured Rats |
| title_sort | autoserum an optimal supplement for bone marrow mesenchymal stem cells of liver injured rats |
| url | http://dx.doi.org/10.1155/2015/459580 |
| work_keys_str_mv | AT qinglinzhang autoserumanoptimalsupplementforbonemarrowmesenchymalstemcellsofliverinjuredrats AT xunsun autoserumanoptimalsupplementforbonemarrowmesenchymalstemcellsofliverinjuredrats AT jianxunding autoserumanoptimalsupplementforbonemarrowmesenchymalstemcellsofliverinjuredrats AT pinghe autoserumanoptimalsupplementforbonemarrowmesenchymalstemcellsofliverinjuredrats AT yujialiu autoserumanoptimalsupplementforbonemarrowmesenchymalstemcellsofliverinjuredrats AT hongjingcheng autoserumanoptimalsupplementforbonemarrowmesenchymalstemcellsofliverinjuredrats AT changlinzhou autoserumanoptimalsupplementforbonemarrowmesenchymalstemcellsofliverinjuredrats AT xiangweimeng autoserumanoptimalsupplementforbonemarrowmesenchymalstemcellsofliverinjuredrats |