Immunophenotypic Characterization of Human Bone Marrow Mast Cells. A Flow Cytometric Study of Normal and Pathological Bone Marrow Samples
The goal of the present paper was to define the immunophenotype of bone marrow mast cells (BMMC) from healthy controls and patients with hematologic malignancies (HM) based on the use of multiple stainings with monoclonal antibodies analyzed by flow cytometry. Our results show that BMMC from both gr...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
1998-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/1998/341340 |
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| author | Luis Escribano Alberto Orfao Jesús Villarrubia Beatriz Díaz-Agustín Carlos Cerveró Agustín Rios José L. Velasco Juana Ciudad José L. Navarro Jesús F. San Miguel |
| author_facet | Luis Escribano Alberto Orfao Jesús Villarrubia Beatriz Díaz-Agustín Carlos Cerveró Agustín Rios José L. Velasco Juana Ciudad José L. Navarro Jesús F. San Miguel |
| author_sort | Luis Escribano |
| collection | DOAJ |
| description | The goal of the present paper was to define the immunophenotype of bone marrow mast cells (BMMC) from healthy controls and patients with hematologic malignancies (HM) based on the use of multiple stainings with monoclonal antibodies analyzed by flow cytometry. Our results show that BMMC from both groups of individuals display a similar but heterogenous immunophenotype. The overall numbers of BMMC are higher in the HM group of individuals (p = 0.08). Three patterns of antigen expression were detected: (1) markers constantly positive in all cases analyzed (CD9, CD29, CD33, CD43, CD44, CD49d, CD49e, CD51, CD71, CD117, and FcεRI), (2) antigens that were constantly negative (CD1a, CD2, CD3, CD5, CD6, CD11a, CD14, CD15, CD16, CD19, CD20, CD21, CD23, CD25, CD30, CD34, CD38, CD41a, CD42b, CD65, CD66b, HLA-DR, and CD138), and (3) markers that were positive in a variable proportion of cases – CD11b (50%), CD11c (77%), CD13 (40%), CD18 (20%), CD22 (68%), CD35 (27%), CD40 (67%), CD54 (88%) and CD61 (40%). In addition, BMMC from all cases explored were CD45+, and this antigen was expressed at an intensity similar to that of mature granulocytes. |
| format | Article |
| id | doaj-art-611e66496ca0487b8ab783330b767bae |
| institution | Kabale University |
| issn | 0921-8912 1878-3651 |
| language | English |
| publishDate | 1998-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-611e66496ca0487b8ab783330b767bae2025-02-03T01:01:45ZengWileyAnalytical Cellular Pathology0921-89121878-36511998-01-0116315115910.1155/1998/341340Immunophenotypic Characterization of Human Bone Marrow Mast Cells. A Flow Cytometric Study of Normal and Pathological Bone Marrow SamplesLuis Escribano0Alberto Orfao1Jesús Villarrubia2Beatriz Díaz-Agustín3Carlos Cerveró4Agustín Rios5José L. Velasco6Juana Ciudad7José L. Navarro8Jesús F. San Miguel9Servicio de Hematología, Hospital Ramón y Cajal, Universidad de Alcalá de Henares, Madrid, SpainServicio Central de Citometría, Universidad de Salamanca, SpainServicio de Hematología, Hospital Ramón y Cajal, Universidad de Alcalá de Henares, Madrid, SpainServicio de Hematología, Hospital Ramón y Cajal, Universidad de Alcalá de Henares, Madrid, SpainServicio de Hematología, Hospital Ramón y Cajal, Universidad de Alcalá de Henares, Madrid, SpainDepartamento de Medicina, Servicio de Hematología, Universidad de Salamanca, SpainServicio de Hematología, Hospital Ramón y Cajal, Universidad de Alcalá de Henares, Madrid, SpainServicio Central de Citometría, Universidad de Salamanca, SpainServicio de Hematología, Hospital Ramón y Cajal, Universidad de Alcalá de Henares, Madrid, SpainDepartamento de Medicina, Servicio de Hematología, Universidad de Salamanca, SpainThe goal of the present paper was to define the immunophenotype of bone marrow mast cells (BMMC) from healthy controls and patients with hematologic malignancies (HM) based on the use of multiple stainings with monoclonal antibodies analyzed by flow cytometry. Our results show that BMMC from both groups of individuals display a similar but heterogenous immunophenotype. The overall numbers of BMMC are higher in the HM group of individuals (p = 0.08). Three patterns of antigen expression were detected: (1) markers constantly positive in all cases analyzed (CD9, CD29, CD33, CD43, CD44, CD49d, CD49e, CD51, CD71, CD117, and FcεRI), (2) antigens that were constantly negative (CD1a, CD2, CD3, CD5, CD6, CD11a, CD14, CD15, CD16, CD19, CD20, CD21, CD23, CD25, CD30, CD34, CD38, CD41a, CD42b, CD65, CD66b, HLA-DR, and CD138), and (3) markers that were positive in a variable proportion of cases – CD11b (50%), CD11c (77%), CD13 (40%), CD18 (20%), CD22 (68%), CD35 (27%), CD40 (67%), CD54 (88%) and CD61 (40%). In addition, BMMC from all cases explored were CD45+, and this antigen was expressed at an intensity similar to that of mature granulocytes.http://dx.doi.org/10.1155/1998/341340 |
| spellingShingle | Luis Escribano Alberto Orfao Jesús Villarrubia Beatriz Díaz-Agustín Carlos Cerveró Agustín Rios José L. Velasco Juana Ciudad José L. Navarro Jesús F. San Miguel Immunophenotypic Characterization of Human Bone Marrow Mast Cells. A Flow Cytometric Study of Normal and Pathological Bone Marrow Samples Analytical Cellular Pathology |
| title | Immunophenotypic Characterization of Human Bone Marrow Mast Cells. A Flow Cytometric Study of Normal and Pathological Bone Marrow Samples |
| title_full | Immunophenotypic Characterization of Human Bone Marrow Mast Cells. A Flow Cytometric Study of Normal and Pathological Bone Marrow Samples |
| title_fullStr | Immunophenotypic Characterization of Human Bone Marrow Mast Cells. A Flow Cytometric Study of Normal and Pathological Bone Marrow Samples |
| title_full_unstemmed | Immunophenotypic Characterization of Human Bone Marrow Mast Cells. A Flow Cytometric Study of Normal and Pathological Bone Marrow Samples |
| title_short | Immunophenotypic Characterization of Human Bone Marrow Mast Cells. A Flow Cytometric Study of Normal and Pathological Bone Marrow Samples |
| title_sort | immunophenotypic characterization of human bone marrow mast cells a flow cytometric study of normal and pathological bone marrow samples |
| url | http://dx.doi.org/10.1155/1998/341340 |
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