The Effects of Subchronic Exposure to Metribuzin on Danio rerio
The aim of this study was to assess the impact of metribuzin in surface waters on fish under experimental conditions. The effects of subchronic exposure to metribuzin on fish growth and the development of histopathological changes in selected organs (gill, kidney, liver) and on activity of some bioc...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/728189 |
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| author | Lucie Plhalova Stanislava Stepanova Eva Praskova Lucie Chromcova Lenka Zelnickova Lenka Divisova Misa Skoric Vladimira Pistekova Iveta Bedanova Zdenka Svobodova |
| author_facet | Lucie Plhalova Stanislava Stepanova Eva Praskova Lucie Chromcova Lenka Zelnickova Lenka Divisova Misa Skoric Vladimira Pistekova Iveta Bedanova Zdenka Svobodova |
| author_sort | Lucie Plhalova |
| collection | DOAJ |
| description | The aim of this study was to assess the impact of metribuzin in surface waters on fish under experimental conditions. The effects of subchronic exposure to metribuzin on fish growth and the development of histopathological changes in selected organs (gill, kidney, liver) and on activity of some biochemical markers (CYP450, EROD) in Danio rerio were investigated during a 28-day toxicity test. Juvenile growth tests were performed on D. rerio according to OECD method number 215. Fish at an initial age of 30 days were exposed to a range of metribuzin concentrations (1.5, 5, 16, 33, and 53 mg L−1). Exposure to metribuzin at 53 mg L−1 was associated with increased mortality. Negative effects with regard to total body weight, length, and the inhibition of specific growth rate were induced at concentrations of 33 and 53 mg L−1. Histopathological examination revealed pathological lesions in the liver in pesticide-exposed fish only at the highest concentration of 53 mg L−1 of metribuzin. Based on the results of growth rate, biochemical markers (CYP450, EROD), and histopathological examination, the lowest observed effect concentration (LOEC) value was 33 mg L−1 and the no observed effect concentration (NOEC) value was 16 mg L−1. |
| format | Article |
| id | doaj-art-611c57d714124dc1964e76b11fd085b9 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-611c57d714124dc1964e76b11fd085b92025-02-03T01:31:26ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/728189728189The Effects of Subchronic Exposure to Metribuzin on Danio rerioLucie Plhalova0Stanislava Stepanova1Eva Praskova2Lucie Chromcova3Lenka Zelnickova4Lenka Divisova5Misa Skoric6Vladimira Pistekova7Iveta Bedanova8Zdenka Svobodova9Department of Veterinary Public Health and Toxicology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1/3, 612 42 Brno, Czech RepublicDepartment of Veterinary Public Health and Toxicology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1/3, 612 42 Brno, Czech RepublicDepartment of Veterinary Public Health and Toxicology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1/3, 612 42 Brno, Czech RepublicDepartment of Veterinary Public Health and Toxicology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1/3, 612 42 Brno, Czech RepublicDepartment of Veterinary Public Health and Toxicology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1/3, 612 42 Brno, Czech RepublicDepartment of Veterinary Public Health and Toxicology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1/3, 612 42 Brno, Czech RepublicDepartment of Pathological Morphology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Czech RepublicDepartment of Veterinary Public Health and Toxicology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1/3, 612 42 Brno, Czech RepublicDepartment of Veterinary Public Health and Toxicology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1/3, 612 42 Brno, Czech RepublicDepartment of Veterinary Public Health and Toxicology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1/3, 612 42 Brno, Czech RepublicThe aim of this study was to assess the impact of metribuzin in surface waters on fish under experimental conditions. The effects of subchronic exposure to metribuzin on fish growth and the development of histopathological changes in selected organs (gill, kidney, liver) and on activity of some biochemical markers (CYP450, EROD) in Danio rerio were investigated during a 28-day toxicity test. Juvenile growth tests were performed on D. rerio according to OECD method number 215. Fish at an initial age of 30 days were exposed to a range of metribuzin concentrations (1.5, 5, 16, 33, and 53 mg L−1). Exposure to metribuzin at 53 mg L−1 was associated with increased mortality. Negative effects with regard to total body weight, length, and the inhibition of specific growth rate were induced at concentrations of 33 and 53 mg L−1. Histopathological examination revealed pathological lesions in the liver in pesticide-exposed fish only at the highest concentration of 53 mg L−1 of metribuzin. Based on the results of growth rate, biochemical markers (CYP450, EROD), and histopathological examination, the lowest observed effect concentration (LOEC) value was 33 mg L−1 and the no observed effect concentration (NOEC) value was 16 mg L−1.http://dx.doi.org/10.1100/2012/728189 |
| spellingShingle | Lucie Plhalova Stanislava Stepanova Eva Praskova Lucie Chromcova Lenka Zelnickova Lenka Divisova Misa Skoric Vladimira Pistekova Iveta Bedanova Zdenka Svobodova The Effects of Subchronic Exposure to Metribuzin on Danio rerio The Scientific World Journal |
| title | The Effects of Subchronic Exposure to Metribuzin on Danio rerio |
| title_full | The Effects of Subchronic Exposure to Metribuzin on Danio rerio |
| title_fullStr | The Effects of Subchronic Exposure to Metribuzin on Danio rerio |
| title_full_unstemmed | The Effects of Subchronic Exposure to Metribuzin on Danio rerio |
| title_short | The Effects of Subchronic Exposure to Metribuzin on Danio rerio |
| title_sort | effects of subchronic exposure to metribuzin on danio rerio |
| url | http://dx.doi.org/10.1100/2012/728189 |
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