Amniotic-Fluid Stem Cells: Growth Dynamics and Differentiation Potential after a CD-117-Based Selection Procedure
Amniotic fluid (AF) has become an interesting source of fetal stem cells. However, AF contains heterogeneous and multiple, partially differentiated cell types. After isolation from the amniotic fluid, cells were characterized regarding their morphology and growth dynamics. They were sorted by magnet...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.4061/2011/715341 |
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| author | S. Arnhold S. Glüer K. Hartmann O. Raabe K. Addicks S. Wenisch M. Hoopmann |
| author_facet | S. Arnhold S. Glüer K. Hartmann O. Raabe K. Addicks S. Wenisch M. Hoopmann |
| author_sort | S. Arnhold |
| collection | DOAJ |
| description | Amniotic fluid (AF) has become an interesting source of fetal stem cells. However, AF contains heterogeneous and multiple, partially differentiated cell types. After isolation from the amniotic fluid, cells were characterized regarding their morphology and growth dynamics. They were sorted by magnetic associated cell sorting using the surface marker CD 117. In order to show stem cell characteristics such as pluripotency and to evaluate a possible therapeutic application of these cells, AF fluid-derived stem cells were differentiated along the adipogenic, osteogenic, and chondrogenic as well as the neuronal lineage under hypoxic conditions. Our findings reveal that magnetic associated cell sorting (MACS) does not markedly influence growth characteristics as demonstrated by the generation doubling time. There was, however, an effect regarding an altered adipogenic, osteogenic, and chondrogenic differentiation capacity in the selected cell fraction. In contrast, in the unselected cell population neuronal differentiation is enhanced. |
| format | Article |
| id | doaj-art-60d11882b9204d599b3b82c80040e348 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-60d11882b9204d599b3b82c80040e3482025-02-03T01:02:52ZengWileyStem Cells International1687-966X1687-96782011-01-01201110.4061/2011/715341715341Amniotic-Fluid Stem Cells: Growth Dynamics and Differentiation Potential after a CD-117-Based Selection ProcedureS. Arnhold0S. Glüer1K. Hartmann2O. Raabe3K. Addicks4S. Wenisch5M. Hoopmann6Department of Veterinary Anatomy, Justus-Liebig-University Giessen, Frankfurter Straße 98, 35392 Giessen, GermanyDepartment of Anatomy I, University of Cologne, 50924 Cologne, GermanyDepartment of Veterinary Anatomy, Justus-Liebig-University Giessen, Frankfurter Straße 98, 35392 Giessen, GermanyDepartment of Veterinary Anatomy, Justus-Liebig-University Giessen, Frankfurter Straße 98, 35392 Giessen, GermanyDepartment of Anatomy I, University of Cologne, 50924 Cologne, GermanyDepartment of Veterinary Anatomy, Justus-Liebig-University Giessen, Frankfurter Straße 98, 35392 Giessen, GermanyClinic for Obstetrics and Gynaecology, University of Tuebingen, 72016 Tuebingen, GermanyAmniotic fluid (AF) has become an interesting source of fetal stem cells. However, AF contains heterogeneous and multiple, partially differentiated cell types. After isolation from the amniotic fluid, cells were characterized regarding their morphology and growth dynamics. They were sorted by magnetic associated cell sorting using the surface marker CD 117. In order to show stem cell characteristics such as pluripotency and to evaluate a possible therapeutic application of these cells, AF fluid-derived stem cells were differentiated along the adipogenic, osteogenic, and chondrogenic as well as the neuronal lineage under hypoxic conditions. Our findings reveal that magnetic associated cell sorting (MACS) does not markedly influence growth characteristics as demonstrated by the generation doubling time. There was, however, an effect regarding an altered adipogenic, osteogenic, and chondrogenic differentiation capacity in the selected cell fraction. In contrast, in the unselected cell population neuronal differentiation is enhanced.http://dx.doi.org/10.4061/2011/715341 |
| spellingShingle | S. Arnhold S. Glüer K. Hartmann O. Raabe K. Addicks S. Wenisch M. Hoopmann Amniotic-Fluid Stem Cells: Growth Dynamics and Differentiation Potential after a CD-117-Based Selection Procedure Stem Cells International |
| title | Amniotic-Fluid Stem Cells: Growth Dynamics and Differentiation Potential after a CD-117-Based Selection Procedure |
| title_full | Amniotic-Fluid Stem Cells: Growth Dynamics and Differentiation Potential after a CD-117-Based Selection Procedure |
| title_fullStr | Amniotic-Fluid Stem Cells: Growth Dynamics and Differentiation Potential after a CD-117-Based Selection Procedure |
| title_full_unstemmed | Amniotic-Fluid Stem Cells: Growth Dynamics and Differentiation Potential after a CD-117-Based Selection Procedure |
| title_short | Amniotic-Fluid Stem Cells: Growth Dynamics and Differentiation Potential after a CD-117-Based Selection Procedure |
| title_sort | amniotic fluid stem cells growth dynamics and differentiation potential after a cd 117 based selection procedure |
| url | http://dx.doi.org/10.4061/2011/715341 |
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