The Effects of BMP-2, miR-31, miR-106a, and miR-148a on Osteogenic Differentiation of MSCs Derived from Amnion in Comparison with MSCs Derived from the Bone Marrow
Mesenchymal stromal cells (MSCs) offering valuable anticipations for the treatment of degenerative diseases. They can be found in many tissues including amnion. MSCs from amnion (AM-MSCs) can differentiate into osteoblast similar to that of bone marrow-derived MSCs (BM-MSCs). However, the ability is...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/7257628 |
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| author | Sirikul Manochantr Kulisara Marupanthorn Chairat Tantrawatpan Pakpoom Kheolamai Duangrat Tantikanlayaporn Prakasit Sanguanjit |
| author_facet | Sirikul Manochantr Kulisara Marupanthorn Chairat Tantrawatpan Pakpoom Kheolamai Duangrat Tantikanlayaporn Prakasit Sanguanjit |
| author_sort | Sirikul Manochantr |
| collection | DOAJ |
| description | Mesenchymal stromal cells (MSCs) offering valuable anticipations for the treatment of degenerative diseases. They can be found in many tissues including amnion. MSCs from amnion (AM-MSCs) can differentiate into osteoblast similar to that of bone marrow-derived MSCs (BM-MSCs). However, the ability is not much efficient compared to BM-MSCs. This study aimed to examine the effects of BMP-2 and miRNAs on osteogenic differentiation of AM-MSCs compared to those of BM-MSCs. The osteogenic differentiation capacity after miRNA treatment was assessed by ALP expression, ALP activity, and osteogenic marker gene expression. The results showed that the osteogenic differentiation capacity increased after BMP-2 treatment both in AM-MSCs and BM-MSCs. MiR-31, miR-106a, and miR-148a were downregulated during the osteogenic differentiation. After transfection with anti-miRNAs, ALP activity and osteogenic genes were increased over the time of differentiation. The data lead to the potential for using AM-MSCs as an alternative source for bone regeneration. Moreover, the information of miRNA expression and function during osteogenic differentiation may be useful for the development of new therapeutics or enhanced an in vitro culture technique required for stem cell-based therapies in the bone regeneration. |
| format | Article |
| id | doaj-art-60b5deb9e2b244c48ea6808f9ebcf8c0 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-60b5deb9e2b244c48ea6808f9ebcf8c02025-02-03T01:03:00ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/72576287257628The Effects of BMP-2, miR-31, miR-106a, and miR-148a on Osteogenic Differentiation of MSCs Derived from Amnion in Comparison with MSCs Derived from the Bone MarrowSirikul Manochantr0Kulisara Marupanthorn1Chairat Tantrawatpan2Pakpoom Kheolamai3Duangrat Tantikanlayaporn4Prakasit Sanguanjit5Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathumthani 12120, ThailandDivision of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathumthani 12120, ThailandDivision of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathumthani 12120, ThailandDivision of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathumthani 12120, ThailandDivision of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathumthani 12120, ThailandDepartment of Orthopedics, Faculty of Medicine, Thammasat University, Pathumthani 12120, ThailandMesenchymal stromal cells (MSCs) offering valuable anticipations for the treatment of degenerative diseases. They can be found in many tissues including amnion. MSCs from amnion (AM-MSCs) can differentiate into osteoblast similar to that of bone marrow-derived MSCs (BM-MSCs). However, the ability is not much efficient compared to BM-MSCs. This study aimed to examine the effects of BMP-2 and miRNAs on osteogenic differentiation of AM-MSCs compared to those of BM-MSCs. The osteogenic differentiation capacity after miRNA treatment was assessed by ALP expression, ALP activity, and osteogenic marker gene expression. The results showed that the osteogenic differentiation capacity increased after BMP-2 treatment both in AM-MSCs and BM-MSCs. MiR-31, miR-106a, and miR-148a were downregulated during the osteogenic differentiation. After transfection with anti-miRNAs, ALP activity and osteogenic genes were increased over the time of differentiation. The data lead to the potential for using AM-MSCs as an alternative source for bone regeneration. Moreover, the information of miRNA expression and function during osteogenic differentiation may be useful for the development of new therapeutics or enhanced an in vitro culture technique required for stem cell-based therapies in the bone regeneration.http://dx.doi.org/10.1155/2017/7257628 |
| spellingShingle | Sirikul Manochantr Kulisara Marupanthorn Chairat Tantrawatpan Pakpoom Kheolamai Duangrat Tantikanlayaporn Prakasit Sanguanjit The Effects of BMP-2, miR-31, miR-106a, and miR-148a on Osteogenic Differentiation of MSCs Derived from Amnion in Comparison with MSCs Derived from the Bone Marrow Stem Cells International |
| title | The Effects of BMP-2, miR-31, miR-106a, and miR-148a on Osteogenic Differentiation of MSCs Derived from Amnion in Comparison with MSCs Derived from the Bone Marrow |
| title_full | The Effects of BMP-2, miR-31, miR-106a, and miR-148a on Osteogenic Differentiation of MSCs Derived from Amnion in Comparison with MSCs Derived from the Bone Marrow |
| title_fullStr | The Effects of BMP-2, miR-31, miR-106a, and miR-148a on Osteogenic Differentiation of MSCs Derived from Amnion in Comparison with MSCs Derived from the Bone Marrow |
| title_full_unstemmed | The Effects of BMP-2, miR-31, miR-106a, and miR-148a on Osteogenic Differentiation of MSCs Derived from Amnion in Comparison with MSCs Derived from the Bone Marrow |
| title_short | The Effects of BMP-2, miR-31, miR-106a, and miR-148a on Osteogenic Differentiation of MSCs Derived from Amnion in Comparison with MSCs Derived from the Bone Marrow |
| title_sort | effects of bmp 2 mir 31 mir 106a and mir 148a on osteogenic differentiation of mscs derived from amnion in comparison with mscs derived from the bone marrow |
| url | http://dx.doi.org/10.1155/2017/7257628 |
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