Evaluation of Apoptosis-Inducing Coumarins Isolated from <i>Peucedanum japonicum</i> Roots: The Potential for Leukemia Treatment via the Mitochondria-Mediated Pathway
Inducing programmed cell death in tumors is a fundamental approach in cancer therapy, prompting extensive efforts to discover bioactive compounds with anticancer properties. <i>Peucedanum japonicum</i>, a plant used in traditional medicine across East Asia, has been reported to exhibit v...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
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| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/13/23/1982 |
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| Summary: | Inducing programmed cell death in tumors is a fundamental approach in cancer therapy, prompting extensive efforts to discover bioactive compounds with anticancer properties. <i>Peucedanum japonicum</i>, a plant used in traditional medicine across East Asia, has been reported to exhibit various biological activities, including anticancer effects. This study aimed to evaluate the apoptosis-inducing effects of methanol/dichloromethane (MeOH/CH<sub>2</sub>Cl<sub>2</sub>) extracts of <i>P. japonicum</i> roots and their components in HL-60 human leukemia cells. Compounds were isolated using solvent extraction and reverse-phase column chromatography, and their structures were confirmed by nuclear magnetic resonance (NMR) spectroscopy. The cytotoxicity effect of the compounds was tested on various cancer cell lines (HL-60, A549, and MCF-7). Two coumarins, (−)-isosamidin (<b>1</b>) and 3′S,4′S-disenecioylkhellactone (<b>2</b>), were isolated through bioactivity-guided fractionation. Compound <b>2</b> significantly induced apoptosis in HL-60 cells, as evidenced by an increase in the sub-G1 cell population and the initiation of both early and late apoptosis. Additional apoptotic markers, including reduced mitochondrial membrane potential (MMP) and increased cleavage of caspase-3, -8, and -9, were observed. These findings suggest that compound <b>2</b> shows potential as a candidate for leukemia treatment, providing a promising natural-product-based approach to cancer therapy. |
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| ISSN: | 2073-4409 |