Dietary Nannochloropsis oculata ameliorates lead acetate induced reproductive toxicity in Barki rams: NF-κB and cytokines pathways
The study aimed to explore the protective effect of Nannochloropsis oculata (N.oculata) on lead induced reproductive toxicity in rams. Sixteen Barki rams were divided equally in to 4 groups; group A: control, group B: was administered 3 % dietary N. oculate, group C: was gavaged 5 mg/kg/day lead ace...
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324015288 |
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| author | Doaa H. Elsayed Sameer A. Nagadi Heba M.A. Abdelrazek Ahmed. S. El‑Hawy Moharram F. El-Bassiony Seham A. Helmy Yasmina K. Mahmoud Ibrahim E. Helal Shady T. Hassan |
| author_facet | Doaa H. Elsayed Sameer A. Nagadi Heba M.A. Abdelrazek Ahmed. S. El‑Hawy Moharram F. El-Bassiony Seham A. Helmy Yasmina K. Mahmoud Ibrahim E. Helal Shady T. Hassan |
| author_sort | Doaa H. Elsayed |
| collection | DOAJ |
| description | The study aimed to explore the protective effect of Nannochloropsis oculata (N.oculata) on lead induced reproductive toxicity in rams. Sixteen Barki rams were divided equally in to 4 groups; group A: control, group B: was administered 3 % dietary N. oculate, group C: was gavaged 5 mg/kg/day lead acetate and group D: was supplemented with 3 % dietary N. oculata and gavaged with 5 mg/kg/day lead acetate. The experiment lasted 180 days. Semen and blood samples were obtained. Ejaculates were examined for semen criteria besides, analysis of testosterone. Testicular oxidant/antioxidant markers, cytokines, gene expression of interleukin-6 (IL-6) and nuclear factor Kappa B (NF-κB) were estimated. Alga-treated rams revealed significant upgrades in semen criteria, serum testosterone and reduced glutathione (GSH); meanwhile, downregulation in malondialdehyde (MDA), IL-4, IL-2 as well as gene expressions of IL-6 and NF-κB as compared to other treated groups. However, lead-treated rams showed significant deteriorations in semen criteria and reduced GSH while significant elevations in MDA, IL-4, IL-2 as well as gene expression of IL-6 and NF-κB were noticed than control. Alga supplementation to lead-intoxicated rams significantly upgraded the lead induced alterations that were manifested by improvement in testicular histopathology. In conclusion, the addition of alga mitigated lead induced reproductive toxicity in rams via improving testosterone, oxidant/antioxidant status, semen criteria and reducing inflammatory cytokines. |
| format | Article |
| id | doaj-art-60886b692ddf4882af69a308c7a4aeb5 |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-60886b692ddf4882af69a308c7a4aeb52025-01-23T05:25:37ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01289117452Dietary Nannochloropsis oculata ameliorates lead acetate induced reproductive toxicity in Barki rams: NF-κB and cytokines pathwaysDoaa H. Elsayed0Sameer A. Nagadi1Heba M.A. Abdelrazek2Ahmed. S. El‑Hawy3Moharram F. El-Bassiony4Seham A. Helmy5Yasmina K. Mahmoud6Ibrahim E. Helal7Shady T. Hassan8Department of Theriogenology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, EgyptDepartment of Agriculture, Faculty of Environment Sciences, King Abdulaziz University, Jeddah 80269, Saudi ArabiaDepartment of Physiology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt; Corresponding author.Animal and Poultry Production Division, Desert Research Center, Cairo, EgyptAnimal and Poultry Production Division, Desert Research Center, Cairo, EgyptDepartment of Cytology and Histology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, EgyptDepartment of Biochemistry, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, EgyptDepartment of Agriculture, Faculty of Environment Sciences, King Abdulaziz University, Jeddah 80269, Saudi Arabia; Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, EgyptDepartment of Theriogenology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, EgyptThe study aimed to explore the protective effect of Nannochloropsis oculata (N.oculata) on lead induced reproductive toxicity in rams. Sixteen Barki rams were divided equally in to 4 groups; group A: control, group B: was administered 3 % dietary N. oculate, group C: was gavaged 5 mg/kg/day lead acetate and group D: was supplemented with 3 % dietary N. oculata and gavaged with 5 mg/kg/day lead acetate. The experiment lasted 180 days. Semen and blood samples were obtained. Ejaculates were examined for semen criteria besides, analysis of testosterone. Testicular oxidant/antioxidant markers, cytokines, gene expression of interleukin-6 (IL-6) and nuclear factor Kappa B (NF-κB) were estimated. Alga-treated rams revealed significant upgrades in semen criteria, serum testosterone and reduced glutathione (GSH); meanwhile, downregulation in malondialdehyde (MDA), IL-4, IL-2 as well as gene expressions of IL-6 and NF-κB as compared to other treated groups. However, lead-treated rams showed significant deteriorations in semen criteria and reduced GSH while significant elevations in MDA, IL-4, IL-2 as well as gene expression of IL-6 and NF-κB were noticed than control. Alga supplementation to lead-intoxicated rams significantly upgraded the lead induced alterations that were manifested by improvement in testicular histopathology. In conclusion, the addition of alga mitigated lead induced reproductive toxicity in rams via improving testosterone, oxidant/antioxidant status, semen criteria and reducing inflammatory cytokines.http://www.sciencedirect.com/science/article/pii/S0147651324015288LeadMicroalgaNannochloropsis oculataSemenToxicity |
| spellingShingle | Doaa H. Elsayed Sameer A. Nagadi Heba M.A. Abdelrazek Ahmed. S. El‑Hawy Moharram F. El-Bassiony Seham A. Helmy Yasmina K. Mahmoud Ibrahim E. Helal Shady T. Hassan Dietary Nannochloropsis oculata ameliorates lead acetate induced reproductive toxicity in Barki rams: NF-κB and cytokines pathways Ecotoxicology and Environmental Safety Lead Microalga Nannochloropsis oculata Semen Toxicity |
| title | Dietary Nannochloropsis oculata ameliorates lead acetate induced reproductive toxicity in Barki rams: NF-κB and cytokines pathways |
| title_full | Dietary Nannochloropsis oculata ameliorates lead acetate induced reproductive toxicity in Barki rams: NF-κB and cytokines pathways |
| title_fullStr | Dietary Nannochloropsis oculata ameliorates lead acetate induced reproductive toxicity in Barki rams: NF-κB and cytokines pathways |
| title_full_unstemmed | Dietary Nannochloropsis oculata ameliorates lead acetate induced reproductive toxicity in Barki rams: NF-κB and cytokines pathways |
| title_short | Dietary Nannochloropsis oculata ameliorates lead acetate induced reproductive toxicity in Barki rams: NF-κB and cytokines pathways |
| title_sort | dietary nannochloropsis oculata ameliorates lead acetate induced reproductive toxicity in barki rams nf κb and cytokines pathways |
| topic | Lead Microalga Nannochloropsis oculata Semen Toxicity |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324015288 |
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