Reinstatement of Morphine-Induced Conditioned Place Preference in Mice by Priming Injections
To construct a model of relapse of drug abuse in mice, the induction, we evaluated the extinction and reinstatement of morphine-induced place preference. In Experiment 1, we examined the effects of morphine (0, 2, 3, 5, 10, 20 and 40 mg/kg) in the conditioned place preference (CPP) paradigm. Mice sh...
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| Format: | Article |
| Language: | English |
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Wiley
2003-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/NP.2003.279 |
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| author | B. Ribeiro Do Couto M. A. Aguilar C. Manzanedo M. Rodríguez-Arias J. Miñarro |
| author_facet | B. Ribeiro Do Couto M. A. Aguilar C. Manzanedo M. Rodríguez-Arias J. Miñarro |
| author_sort | B. Ribeiro Do Couto |
| collection | DOAJ |
| description | To construct a model of relapse of drug
abuse in mice, the induction, we evaluated the
extinction and reinstatement of morphine-induced
place preference. In Experiment 1, we
examined the effects of morphine (0, 2, 3, 5, 10,
20 and 40 mg/kg) in the conditioned place
preference (CPP) paradigm. Mice showed CPP
with 5, 10, 20 and 40 mg/kg. In Experiment 2,
we evaluated the effects of two different extinction
procedures. After conditioning with 40
mg/kg of morphine, the mice underwent daily
extinction sessions of 60 or 15 min of duration.
CPP was extinguished after seven and nine
sessions, respectively. In Experiment 3, we
tested the reinstating effects of several priming
doses of morphine. Mice were conditioned with
40 mg/kg of morphine and underwent the daily
15 min extinction sessions until CPP was no
longer evident. Then, the effects of morphine (0,
2, 3, 5, 10, 20, 40 mg/kg, i.p.) were evaluated.
CPP was reinstated by doses from 5 mg/kg
upward. The results show that morphine priming
injections are effective in reactivating opiateseeking
behavior in mice, and thus, the CPP
paradigm might be useful to investigate the
mechanisms underlying relapse of drug abuse. |
| format | Article |
| id | doaj-art-607ee68a07774b118a3e578b780ebd10 |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2003-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-607ee68a07774b118a3e578b780ebd102025-02-03T01:26:48ZengWileyNeural Plasticity2090-59041687-54432003-01-0110427929010.1155/NP.2003.279Reinstatement of Morphine-Induced Conditioned Place Preference in Mice by Priming InjectionsB. Ribeiro Do Couto0M. A. Aguilar1C. Manzanedo2M. Rodríguez-Arias3J. Miñarro4Departamento de Psicobiología, Facultad de Psicología, Universitat de Valencia, Avda. Blasco Ibáñez, Valencia 21. 46010, SpainDepartamento de Psicobiología, Facultad de Psicología, Universitat de Valencia, Avda. Blasco Ibáñez, Valencia 21. 46010, SpainDepartamento de Psicobiología, Facultad de Psicología, Universitat de Valencia, Avda. Blasco Ibáñez, Valencia 21. 46010, SpainDepartamento de Psicobiología, Facultad de Psicología, Universitat de Valencia, Avda. Blasco Ibáñez, Valencia 21. 46010, SpainDepartamento de Psicobiología, Facultad de Psicología, Universitat de Valencia, Avda. Blasco Ibáñez, Valencia 21. 46010, SpainTo construct a model of relapse of drug abuse in mice, the induction, we evaluated the extinction and reinstatement of morphine-induced place preference. In Experiment 1, we examined the effects of morphine (0, 2, 3, 5, 10, 20 and 40 mg/kg) in the conditioned place preference (CPP) paradigm. Mice showed CPP with 5, 10, 20 and 40 mg/kg. In Experiment 2, we evaluated the effects of two different extinction procedures. After conditioning with 40 mg/kg of morphine, the mice underwent daily extinction sessions of 60 or 15 min of duration. CPP was extinguished after seven and nine sessions, respectively. In Experiment 3, we tested the reinstating effects of several priming doses of morphine. Mice were conditioned with 40 mg/kg of morphine and underwent the daily 15 min extinction sessions until CPP was no longer evident. Then, the effects of morphine (0, 2, 3, 5, 10, 20, 40 mg/kg, i.p.) were evaluated. CPP was reinstated by doses from 5 mg/kg upward. The results show that morphine priming injections are effective in reactivating opiateseeking behavior in mice, and thus, the CPP paradigm might be useful to investigate the mechanisms underlying relapse of drug abuse.http://dx.doi.org/10.1155/NP.2003.279 |
| spellingShingle | B. Ribeiro Do Couto M. A. Aguilar C. Manzanedo M. Rodríguez-Arias J. Miñarro Reinstatement of Morphine-Induced Conditioned Place Preference in Mice by Priming Injections Neural Plasticity |
| title | Reinstatement of Morphine-Induced Conditioned
Place Preference in Mice by Priming Injections |
| title_full | Reinstatement of Morphine-Induced Conditioned
Place Preference in Mice by Priming Injections |
| title_fullStr | Reinstatement of Morphine-Induced Conditioned
Place Preference in Mice by Priming Injections |
| title_full_unstemmed | Reinstatement of Morphine-Induced Conditioned
Place Preference in Mice by Priming Injections |
| title_short | Reinstatement of Morphine-Induced Conditioned
Place Preference in Mice by Priming Injections |
| title_sort | reinstatement of morphine induced conditioned place preference in mice by priming injections |
| url | http://dx.doi.org/10.1155/NP.2003.279 |
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