A Comparative Cytotoxic Evaluation of Acrylamide and Diacetone Acrylamide to Investigate Their Suitability for Holographic Photopolymer Formulations

The comparative cytotoxic evaluation of two monomers, diacetone acrylamide (DA) and acrylamide (AA) used in holographic photopolymer formulations, is reported. Two normal cell lines were used: BEAS-2B and HaCaT. Cellular viability was assessed using the MTT assay for three different exposure times....

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Main Authors: Dervil Cody, Alan Casey, Izabela Naydenova, Emilia Mihaylova
Format: Article
Language:English
Published: Wiley 2013-01-01
Series:International Journal of Polymer Science
Online Access:http://dx.doi.org/10.1155/2013/564319
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author Dervil Cody
Alan Casey
Izabela Naydenova
Emilia Mihaylova
author_facet Dervil Cody
Alan Casey
Izabela Naydenova
Emilia Mihaylova
author_sort Dervil Cody
collection DOAJ
description The comparative cytotoxic evaluation of two monomers, diacetone acrylamide (DA) and acrylamide (AA) used in holographic photopolymer formulations, is reported. Two normal cell lines were used: BEAS-2B and HaCaT. Cellular viability was assessed using the MTT assay for three different exposure times. A difference of two orders of magnitude is observed in the lethal dose (LD50) concentrations of the two monomers. Diacetone acrylamide exhibits a significantly lower toxicity profile in comparison to acrylamide at all exposure times. This result justifies the replacement of acrylamide with diacetone acrylamide in the photopolymer formulation, with the view to reducing occupational hazard risks for large-scale holographic device fabrication. A comparative study investigating the holographic recording ability of the two photopolymers in transmission mode showed that the DA photopolymer is capable of reaching refractive index modulation values of 3.3×10-3, which is 80% of the refractive index modulation achieved by the AA photopolymer. This makes the DA-based photopolymers suitable for a wide range of applications.
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institution Kabale University
issn 1687-9422
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language English
publishDate 2013-01-01
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series International Journal of Polymer Science
spelling doaj-art-607c01912bcd4ebe80c7d90fea7e272a2025-02-03T06:15:12ZengWileyInternational Journal of Polymer Science1687-94221687-94302013-01-01201310.1155/2013/564319564319A Comparative Cytotoxic Evaluation of Acrylamide and Diacetone Acrylamide to Investigate Their Suitability for Holographic Photopolymer FormulationsDervil Cody0Alan Casey1Izabela Naydenova2Emilia Mihaylova3Centre for Industrial and Engineering Optics, Dublin Institute of Technology, Dublin 8, IrelandSchool of Physics, College of Sciences and Health, Dublin Institute of Technology, Dublin 8, IrelandCentre for Industrial and Engineering Optics, Dublin Institute of Technology, Dublin 8, IrelandCentre for Industrial and Engineering Optics, Dublin Institute of Technology, Dublin 8, IrelandThe comparative cytotoxic evaluation of two monomers, diacetone acrylamide (DA) and acrylamide (AA) used in holographic photopolymer formulations, is reported. Two normal cell lines were used: BEAS-2B and HaCaT. Cellular viability was assessed using the MTT assay for three different exposure times. A difference of two orders of magnitude is observed in the lethal dose (LD50) concentrations of the two monomers. Diacetone acrylamide exhibits a significantly lower toxicity profile in comparison to acrylamide at all exposure times. This result justifies the replacement of acrylamide with diacetone acrylamide in the photopolymer formulation, with the view to reducing occupational hazard risks for large-scale holographic device fabrication. A comparative study investigating the holographic recording ability of the two photopolymers in transmission mode showed that the DA photopolymer is capable of reaching refractive index modulation values of 3.3×10-3, which is 80% of the refractive index modulation achieved by the AA photopolymer. This makes the DA-based photopolymers suitable for a wide range of applications.http://dx.doi.org/10.1155/2013/564319
spellingShingle Dervil Cody
Alan Casey
Izabela Naydenova
Emilia Mihaylova
A Comparative Cytotoxic Evaluation of Acrylamide and Diacetone Acrylamide to Investigate Their Suitability for Holographic Photopolymer Formulations
International Journal of Polymer Science
title A Comparative Cytotoxic Evaluation of Acrylamide and Diacetone Acrylamide to Investigate Their Suitability for Holographic Photopolymer Formulations
title_full A Comparative Cytotoxic Evaluation of Acrylamide and Diacetone Acrylamide to Investigate Their Suitability for Holographic Photopolymer Formulations
title_fullStr A Comparative Cytotoxic Evaluation of Acrylamide and Diacetone Acrylamide to Investigate Their Suitability for Holographic Photopolymer Formulations
title_full_unstemmed A Comparative Cytotoxic Evaluation of Acrylamide and Diacetone Acrylamide to Investigate Their Suitability for Holographic Photopolymer Formulations
title_short A Comparative Cytotoxic Evaluation of Acrylamide and Diacetone Acrylamide to Investigate Their Suitability for Holographic Photopolymer Formulations
title_sort comparative cytotoxic evaluation of acrylamide and diacetone acrylamide to investigate their suitability for holographic photopolymer formulations
url http://dx.doi.org/10.1155/2013/564319
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