Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells
In breast carcinoma, disruption of the p53 pathway is one of the most common genetic alterations. The observation that the p53 can express multiple protein isoforms adds a novel level of complexity to the outcome of p53 mutations. p53 expression was analysed by Western immunoblotting and immunohisto...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/618698 |
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| author | Zorka Milićević Vladan Bajić Lada Živković Jelena Kasapović Uroš Andjelković Biljana Spremo-Potparević |
| author_facet | Zorka Milićević Vladan Bajić Lada Živković Jelena Kasapović Uroš Andjelković Biljana Spremo-Potparević |
| author_sort | Zorka Milićević |
| collection | DOAJ |
| description | In breast carcinoma, disruption of the p53 pathway is one of the most common genetic alterations. The observation that the p53 can express multiple protein isoforms adds a novel level of complexity to the outcome of p53 mutations. p53 expression was analysed by Western immunoblotting and immunohistochemistry using monoclonal antibodies DO-7, Pab240, and polyclonal antiserum CM-1. The more frequently p53-positive nuclear staining has been found in the invasive breast tumors. One of the most intriguing findings is that mutant p53 appears as discrete dot-shaped regions within the nucleus of breast cancer cells. In many malignant cells, the nucleolar sequestration of p53 is evident. These observations support the view that the nucleolus is involved directly in the mediation of p53 function or indirectly by the control of the localization of p53 interplayers. p53 expressed in the nuclear fraction of breast cancer cells revealed a wide spectrum of isoforms. p53 isoforms ΔNp53 (47 kDa) and Δ133p53β (35 kDa), known as dominant-negative repressors of p53 function, were detected as the most predominant variants in nuclei of invasive breast carcinoma cells. The isoforms expressed also varied between individual tumors, indicating potential roles of these p53 variants in human breast cancer. |
| format | Article |
| id | doaj-art-6048fca83a02459690c5ddccccf5ee45 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-6048fca83a02459690c5ddccccf5ee452025-02-03T01:00:00ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/618698618698Identification of p53 and Its Isoforms in Human Breast Carcinoma CellsZorka Milićević0Vladan Bajić1Lada Živković2Jelena Kasapović3Uroš Andjelković4Biljana Spremo-Potparević5Laboratory of Molecular Biology and Endocrinology, “Vinča” Institute of Nuclear Sciences, University of Belgrade, Mihaila Petrovića Alasa 12-14, P.O. Box 522, 11 001 Belgrade, SerbiaBiomedical Department, Institute for Pharmaceutical Research and Development, Galenika a.d., Pasterova 2, 11 000 Belgrade, SerbiaDepartment of Biology and Human Genetics, Institute of Physiology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11 000 Belgrade, SerbiaLaboratory of Molecular Biology and Endocrinology, “Vinča” Institute of Nuclear Sciences, University of Belgrade, Mihaila Petrovića Alasa 12-14, P.O. Box 522, 11 001 Belgrade, SerbiaDepartment of Chemistry, Institute for Chemistry, Technology and Metallurgy, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, SerbiaDepartment of Biology and Human Genetics, Institute of Physiology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11 000 Belgrade, SerbiaIn breast carcinoma, disruption of the p53 pathway is one of the most common genetic alterations. The observation that the p53 can express multiple protein isoforms adds a novel level of complexity to the outcome of p53 mutations. p53 expression was analysed by Western immunoblotting and immunohistochemistry using monoclonal antibodies DO-7, Pab240, and polyclonal antiserum CM-1. The more frequently p53-positive nuclear staining has been found in the invasive breast tumors. One of the most intriguing findings is that mutant p53 appears as discrete dot-shaped regions within the nucleus of breast cancer cells. In many malignant cells, the nucleolar sequestration of p53 is evident. These observations support the view that the nucleolus is involved directly in the mediation of p53 function or indirectly by the control of the localization of p53 interplayers. p53 expressed in the nuclear fraction of breast cancer cells revealed a wide spectrum of isoforms. p53 isoforms ΔNp53 (47 kDa) and Δ133p53β (35 kDa), known as dominant-negative repressors of p53 function, were detected as the most predominant variants in nuclei of invasive breast carcinoma cells. The isoforms expressed also varied between individual tumors, indicating potential roles of these p53 variants in human breast cancer.http://dx.doi.org/10.1155/2014/618698 |
| spellingShingle | Zorka Milićević Vladan Bajić Lada Živković Jelena Kasapović Uroš Andjelković Biljana Spremo-Potparević Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells The Scientific World Journal |
| title | Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells |
| title_full | Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells |
| title_fullStr | Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells |
| title_full_unstemmed | Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells |
| title_short | Identification of p53 and Its Isoforms in Human Breast Carcinoma Cells |
| title_sort | identification of p53 and its isoforms in human breast carcinoma cells |
| url | http://dx.doi.org/10.1155/2014/618698 |
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