High-throughput Identification of Phage-derived Imaging Agents
The use of phage-displayed peptide libraries is a powerful method for selecting peptides with desired binding properties. However, the validation and prioritization of “hits” obtained from this screening approach remains challenging. Here, we describe the development and testing of a new analysis me...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2006-01-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2006.00003 |
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| _version_ | 1832544615161397248 |
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| author | Kimberly A. Kelly Paul A. Clemons Amy M. Yu Ralph Weissleder |
| author_facet | Kimberly A. Kelly Paul A. Clemons Amy M. Yu Ralph Weissleder |
| author_sort | Kimberly A. Kelly |
| collection | DOAJ |
| description | The use of phage-displayed peptide libraries is a powerful method for selecting peptides with desired binding properties. However, the validation and prioritization of “hits” obtained from this screening approach remains challenging. Here, we describe the development and testing of a new analysis method to identify and display hits from phage-display experiments and high-throughput enzyme-linked immunosorbent assay screens. We test the method using a phage screen against activated macrophages to develop imaging agents with higher specificity for active disease processes. The new methodology should be useful in identifying phage hits and is extendable to other library screening methods such as small-molecule and nanoparticle libraries. |
| format | Article |
| id | doaj-art-5ff7768cefa444f38d2ae8947658f792 |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2006-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-5ff7768cefa444f38d2ae8947658f7922025-02-03T10:08:00ZengSAGE PublishingMolecular Imaging1536-01212006-01-01510.2310/7290.2006.0000310.2310_7290.2006.00003High-throughput Identification of Phage-derived Imaging AgentsKimberly A. Kelly0Paul A. Clemons1Amy M. Yu2Ralph Weissleder3Massachusetts General Hospital and Harvard Medical School, USAThe Eli & Edythe Broad Institute of Harvard & MIT, USAMassachusetts General Hospital and Harvard Medical School, USAThe Eli & Edythe Broad Institute of Harvard & MIT, USAThe use of phage-displayed peptide libraries is a powerful method for selecting peptides with desired binding properties. However, the validation and prioritization of “hits” obtained from this screening approach remains challenging. Here, we describe the development and testing of a new analysis method to identify and display hits from phage-display experiments and high-throughput enzyme-linked immunosorbent assay screens. We test the method using a phage screen against activated macrophages to develop imaging agents with higher specificity for active disease processes. The new methodology should be useful in identifying phage hits and is extendable to other library screening methods such as small-molecule and nanoparticle libraries.https://doi.org/10.2310/7290.2006.00003 |
| spellingShingle | Kimberly A. Kelly Paul A. Clemons Amy M. Yu Ralph Weissleder High-throughput Identification of Phage-derived Imaging Agents Molecular Imaging |
| title | High-throughput Identification of Phage-derived Imaging Agents |
| title_full | High-throughput Identification of Phage-derived Imaging Agents |
| title_fullStr | High-throughput Identification of Phage-derived Imaging Agents |
| title_full_unstemmed | High-throughput Identification of Phage-derived Imaging Agents |
| title_short | High-throughput Identification of Phage-derived Imaging Agents |
| title_sort | high throughput identification of phage derived imaging agents |
| url | https://doi.org/10.2310/7290.2006.00003 |
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