Metabolic Alterations of the Zebrafish Brain after Acute Alcohol Treatment by 1H Nuclear Magnetic Resonance Spectroscopy
The purpose of this study is to investigate the metabolic alterations associated with acute alcohol treatment in zebrafish by 1H nuclear magnetic resonance spectroscopy (NMRS). The brain metabolism of zebrafish was investigated after acute alcohol treatment (one-hour long exposure of adult fish to 0...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Spectroscopy |
| Online Access: | http://dx.doi.org/10.1155/2013/971914 |
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| author | Dong-Cheol Woo Goo-Young Kim Hyun-Ju Kim Eunjung Bang Hyang-Shuk Rhim Sang-Young Kim Do-Wan Lee Chi-Bong Choi Youl-Hun Seoung Bo-Young Choe |
| author_facet | Dong-Cheol Woo Goo-Young Kim Hyun-Ju Kim Eunjung Bang Hyang-Shuk Rhim Sang-Young Kim Do-Wan Lee Chi-Bong Choi Youl-Hun Seoung Bo-Young Choe |
| author_sort | Dong-Cheol Woo |
| collection | DOAJ |
| description | The purpose of this study is to investigate the metabolic alterations associated with acute alcohol treatment in zebrafish by 1H nuclear magnetic resonance spectroscopy (NMRS). The brain metabolism of zebrafish was investigated after acute alcohol treatment (one-hour long exposure of adult fish to 0.00%, 0.25%, 0.50%, or 1.00% ethyl alcohol) with whole brain extraction. The results of this study showed that glutamate (Glu) was significantly decreased, scyllo-inositol (sIns) showed a small apparent increase only in the highest acute treatment dose group, and myoinositol (mIns) showed a significant decrease. [Glu]/[tCr] and [mIns]/[tCr] levels were significantly reduced regardless of the alcohol dose, and [sIns]/[tCr] was increased in the highest alcohol treatment dose group. The present NMR study revealed that specific metabolites, such as Glu and mIns, were substantially decreased in case of acute alcohol exposed zebrafish brain. |
| format | Article |
| id | doaj-art-5fedbb3c250649f89fc4e25f713f70c8 |
| institution | Kabale University |
| issn | 2314-4920 2314-4939 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Spectroscopy |
| spelling | doaj-art-5fedbb3c250649f89fc4e25f713f70c82025-02-03T05:46:29ZengWileyJournal of Spectroscopy2314-49202314-49392013-01-01201310.1155/2013/971914971914Metabolic Alterations of the Zebrafish Brain after Acute Alcohol Treatment by 1H Nuclear Magnetic Resonance SpectroscopyDong-Cheol Woo0Goo-Young Kim1Hyun-Ju Kim2Eunjung Bang3Hyang-Shuk Rhim4Sang-Young Kim5Do-Wan Lee6Chi-Bong Choi7Youl-Hun Seoung8Bo-Young Choe9Department of Radiology, UT Southwestern Medical Center, Dallas, TX, USADepartment of Molecular Biology, College of Medicine, The Catholic University of Korea, Republic of KoreaEnvironment & Metabolomics Research Team, Seoul Center, Korea Basic Science Institute, Seoul, Republic of KoreaEnvironment & Metabolomics Research Team, Seoul Center, Korea Basic Science Institute, Seoul, Republic of KoreaDepartment of Molecular Biology, College of Medicine, The Catholic University of Korea, Republic of KoreaDepartment of Biomedical Engineering, Research Institute of Biomedical Engineering, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of KoreaDepartment of Biomedical Engineering, Research Institute of Biomedical Engineering, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of KoreaDepartment of Biomedical Engineering, Research Institute of Biomedical Engineering, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of KoreaDepartment of Biomedical Engineering, Research Institute of Biomedical Engineering, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of KoreaDepartment of Biomedical Engineering, Research Institute of Biomedical Engineering, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of KoreaThe purpose of this study is to investigate the metabolic alterations associated with acute alcohol treatment in zebrafish by 1H nuclear magnetic resonance spectroscopy (NMRS). The brain metabolism of zebrafish was investigated after acute alcohol treatment (one-hour long exposure of adult fish to 0.00%, 0.25%, 0.50%, or 1.00% ethyl alcohol) with whole brain extraction. The results of this study showed that glutamate (Glu) was significantly decreased, scyllo-inositol (sIns) showed a small apparent increase only in the highest acute treatment dose group, and myoinositol (mIns) showed a significant decrease. [Glu]/[tCr] and [mIns]/[tCr] levels were significantly reduced regardless of the alcohol dose, and [sIns]/[tCr] was increased in the highest alcohol treatment dose group. The present NMR study revealed that specific metabolites, such as Glu and mIns, were substantially decreased in case of acute alcohol exposed zebrafish brain.http://dx.doi.org/10.1155/2013/971914 |
| spellingShingle | Dong-Cheol Woo Goo-Young Kim Hyun-Ju Kim Eunjung Bang Hyang-Shuk Rhim Sang-Young Kim Do-Wan Lee Chi-Bong Choi Youl-Hun Seoung Bo-Young Choe Metabolic Alterations of the Zebrafish Brain after Acute Alcohol Treatment by 1H Nuclear Magnetic Resonance Spectroscopy Journal of Spectroscopy |
| title | Metabolic Alterations of the Zebrafish Brain after Acute Alcohol Treatment by 1H Nuclear Magnetic Resonance Spectroscopy |
| title_full | Metabolic Alterations of the Zebrafish Brain after Acute Alcohol Treatment by 1H Nuclear Magnetic Resonance Spectroscopy |
| title_fullStr | Metabolic Alterations of the Zebrafish Brain after Acute Alcohol Treatment by 1H Nuclear Magnetic Resonance Spectroscopy |
| title_full_unstemmed | Metabolic Alterations of the Zebrafish Brain after Acute Alcohol Treatment by 1H Nuclear Magnetic Resonance Spectroscopy |
| title_short | Metabolic Alterations of the Zebrafish Brain after Acute Alcohol Treatment by 1H Nuclear Magnetic Resonance Spectroscopy |
| title_sort | metabolic alterations of the zebrafish brain after acute alcohol treatment by 1h nuclear magnetic resonance spectroscopy |
| url | http://dx.doi.org/10.1155/2013/971914 |
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