Stability Indicating Analytical Method Development and Validation of Efavirenz Quantification by High Performance Liquid Chromatographic Technique
A stability-indicating high performance liquid chromatography (HPLC) method is developed for the quantification of efavirenz drug substance. Selected mobile phase is a combination of 50 volume of acetonitrile and 50 volume of 0.86% w/v solution of ammonium dihydrogen phosphate, pH adjusted to 3.0 wi...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2011/605381 |
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| _version_ | 1832564476742729728 |
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| author | R. Kumar M. Sharma G. R. Verma |
| author_facet | R. Kumar M. Sharma G. R. Verma |
| author_sort | R. Kumar |
| collection | DOAJ |
| description | A stability-indicating high performance liquid chromatography (HPLC) method is developed for the quantification of efavirenz drug substance. Selected mobile phase is a combination of 50 volume of acetonitrile and 50 volume of 0.86% w/v solution of ammonium dihydrogen phosphate, pH adjusted to 3.0 with orthophosphoric acid. Optimized column is a stainless steel column packed with base deactivated octa decylsilyl silica gel and at 252 nm wavelength. Linearity of the method is found 40 μg/mL to 160 μg/mL with regression coefficient of 0.9995. The method is validated according to ICH guidelines. |
| format | Article |
| id | doaj-art-5fecfb9bd7e24c4db32333a8c31c9fef |
| institution | Kabale University |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-5fecfb9bd7e24c4db32333a8c31c9fef2025-02-03T01:10:58ZengWileyE-Journal of Chemistry0973-49452090-98102011-01-01841498150310.1155/2011/605381Stability Indicating Analytical Method Development and Validation of Efavirenz Quantification by High Performance Liquid Chromatographic TechniqueR. Kumar0M. Sharma1G. R. Verma2Department of Chemistry, S. D. (P.G.) College Muzaffarnagar 250001 (U.P.), IndiaDepartment of Pure and Applied Chemistry, M.D.S. University, Ajmer (Rajasthan), IndiaDepartment of Chemistry, S. D. (P.G.) College Muzaffarnagar 250001 (U.P.), IndiaA stability-indicating high performance liquid chromatography (HPLC) method is developed for the quantification of efavirenz drug substance. Selected mobile phase is a combination of 50 volume of acetonitrile and 50 volume of 0.86% w/v solution of ammonium dihydrogen phosphate, pH adjusted to 3.0 with orthophosphoric acid. Optimized column is a stainless steel column packed with base deactivated octa decylsilyl silica gel and at 252 nm wavelength. Linearity of the method is found 40 μg/mL to 160 μg/mL with regression coefficient of 0.9995. The method is validated according to ICH guidelines.http://dx.doi.org/10.1155/2011/605381 |
| spellingShingle | R. Kumar M. Sharma G. R. Verma Stability Indicating Analytical Method Development and Validation of Efavirenz Quantification by High Performance Liquid Chromatographic Technique E-Journal of Chemistry |
| title | Stability Indicating Analytical Method Development and Validation of Efavirenz Quantification by High Performance Liquid Chromatographic Technique |
| title_full | Stability Indicating Analytical Method Development and Validation of Efavirenz Quantification by High Performance Liquid Chromatographic Technique |
| title_fullStr | Stability Indicating Analytical Method Development and Validation of Efavirenz Quantification by High Performance Liquid Chromatographic Technique |
| title_full_unstemmed | Stability Indicating Analytical Method Development and Validation of Efavirenz Quantification by High Performance Liquid Chromatographic Technique |
| title_short | Stability Indicating Analytical Method Development and Validation of Efavirenz Quantification by High Performance Liquid Chromatographic Technique |
| title_sort | stability indicating analytical method development and validation of efavirenz quantification by high performance liquid chromatographic technique |
| url | http://dx.doi.org/10.1155/2011/605381 |
| work_keys_str_mv | AT rkumar stabilityindicatinganalyticalmethoddevelopmentandvalidationofefavirenzquantificationbyhighperformanceliquidchromatographictechnique AT msharma stabilityindicatinganalyticalmethoddevelopmentandvalidationofefavirenzquantificationbyhighperformanceliquidchromatographictechnique AT grverma stabilityindicatinganalyticalmethoddevelopmentandvalidationofefavirenzquantificationbyhighperformanceliquidchromatographictechnique |