Chronic restraint stress affects the diurnal rhythms of gut microbial composition and metabolism in a mouse model of depression
Abstract Background Depression is a common mental disorder accompanied by gut microbiota dysbiosis, which disturbs the metabolism of the host. While diurnal oscillation of the intestinal microbiota is involved in regulating host metabolism, the characteristics of the intestinal microbial circadian r...
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BMC
2025-01-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-025-03764-4 |
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| author | Yue Wang Peijin Cui Maolin Cao Ling Ai Li Zeng Xue Li Dan Chen Fang Gong Liang Fang Chanjuan Zhou |
| author_facet | Yue Wang Peijin Cui Maolin Cao Ling Ai Li Zeng Xue Li Dan Chen Fang Gong Liang Fang Chanjuan Zhou |
| author_sort | Yue Wang |
| collection | DOAJ |
| description | Abstract Background Depression is a common mental disorder accompanied by gut microbiota dysbiosis, which disturbs the metabolism of the host. While diurnal oscillation of the intestinal microbiota is involved in regulating host metabolism, the characteristics of the intestinal microbial circadian rhythm in depression remain unknown. Our aim was to investigate the microbial circadian oscillation signature and related metabolic pathways in a mouse model with depression-like behaviours. Methods Chronic restraint stress (CRS) was used to induce depressive-like behaviours in C57BL/6J mice. The open field test (OFT) and forced swimming test (FST) were used to evaluate anxiety- and depressive-like behaviours in the control and CRS groups. Afterwards, faecal samples from the two groups were collected every four hours from ZT2 (9:00 am) to ZT22 (5:00 am). Faecal 16 S rRNA gene sequencing and metabolomics analysis were performed, and the microbial circadian rhythm was analysed via the MetaCycle package in R/RStudio. Results CRS mice exhibited depressive-like behaviours after 4 weeks of restriction. Alpha- and beta-diversity analyses revealed that the microbial composition in control and CRS mice oscillated throughout the day. The circadian rhythm analyses revealed that at the phylum level, Bacteroidota, Firmicutes, Cyanobacteria and Patescibacteria showed circadian rhythmicity in the CRS group. At the genus level, Dubosiella and Romboutsia showed circadian rhythmicity in the control group, and Dubosiella abundance was correlated with tryptophan and galactose metabolism. In the CRS group, Bacteroides, Parabacteroides, and Rikenellaceae_RC9_gut_group showed circadian rhythmicity; among these genera, Parabacteroides was related to tryptophan metabolism, axon regeneration, phenylalanine metabolism and tyrosine metabolism. Conclusion Our data highlight the importance of observing the diurnal oscillation of the microbiome in host with depressive-like states. Rhythmicity in the microbiome may affect the host by regulating distinct metabolic pathways during the light and dark phases. A better combination of microbiota composition and oscillation would help to offer novel insight into key genera and their potential effects on depression. |
| format | Article |
| id | doaj-art-5fd9f96a467d42819d2797884154b498 |
| institution | Kabale University |
| issn | 1471-2180 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj-art-5fd9f96a467d42819d2797884154b4982025-01-26T12:17:55ZengBMCBMC Microbiology1471-21802025-01-0125111210.1186/s12866-025-03764-4Chronic restraint stress affects the diurnal rhythms of gut microbial composition and metabolism in a mouse model of depressionYue Wang0Peijin Cui1Maolin Cao2Ling Ai3Li Zeng4Xue Li5Dan Chen6Fang Gong7Liang Fang8Chanjuan Zhou9Department of Pediatric, Yongchuan Hospital of Chongqing Medical UniversityDepartment of Neurology, Yongchuan Hospital of Chongqing Medical UniversityDepartment of General Practice, Yongchuan Hospital of Chongqing Medical UniversityDepartment of General Practice, Yongchuan Hospital of Chongqing Medical UniversityDepartment of Nephrology, The Second Affiliated Hospital of Chongqing Medical UniversityCentral Laboratory, Yongchuan Hospital of Chongqing Medical UniversityCentral Laboratory, Yongchuan Hospital of Chongqing Medical UniversityDepartment of Pediatric, Yongchuan Hospital of Chongqing Medical UniversityDepartment of Neurology, Yongchuan Hospital of Chongqing Medical UniversityDepartment of Neurology, Yongchuan Hospital of Chongqing Medical UniversityAbstract Background Depression is a common mental disorder accompanied by gut microbiota dysbiosis, which disturbs the metabolism of the host. While diurnal oscillation of the intestinal microbiota is involved in regulating host metabolism, the characteristics of the intestinal microbial circadian rhythm in depression remain unknown. Our aim was to investigate the microbial circadian oscillation signature and related metabolic pathways in a mouse model with depression-like behaviours. Methods Chronic restraint stress (CRS) was used to induce depressive-like behaviours in C57BL/6J mice. The open field test (OFT) and forced swimming test (FST) were used to evaluate anxiety- and depressive-like behaviours in the control and CRS groups. Afterwards, faecal samples from the two groups were collected every four hours from ZT2 (9:00 am) to ZT22 (5:00 am). Faecal 16 S rRNA gene sequencing and metabolomics analysis were performed, and the microbial circadian rhythm was analysed via the MetaCycle package in R/RStudio. Results CRS mice exhibited depressive-like behaviours after 4 weeks of restriction. Alpha- and beta-diversity analyses revealed that the microbial composition in control and CRS mice oscillated throughout the day. The circadian rhythm analyses revealed that at the phylum level, Bacteroidota, Firmicutes, Cyanobacteria and Patescibacteria showed circadian rhythmicity in the CRS group. At the genus level, Dubosiella and Romboutsia showed circadian rhythmicity in the control group, and Dubosiella abundance was correlated with tryptophan and galactose metabolism. In the CRS group, Bacteroides, Parabacteroides, and Rikenellaceae_RC9_gut_group showed circadian rhythmicity; among these genera, Parabacteroides was related to tryptophan metabolism, axon regeneration, phenylalanine metabolism and tyrosine metabolism. Conclusion Our data highlight the importance of observing the diurnal oscillation of the microbiome in host with depressive-like states. Rhythmicity in the microbiome may affect the host by regulating distinct metabolic pathways during the light and dark phases. A better combination of microbiota composition and oscillation would help to offer novel insight into key genera and their potential effects on depression.https://doi.org/10.1186/s12866-025-03764-4DepressionGut microbiotaCircadian rhythmsMetabolism |
| spellingShingle | Yue Wang Peijin Cui Maolin Cao Ling Ai Li Zeng Xue Li Dan Chen Fang Gong Liang Fang Chanjuan Zhou Chronic restraint stress affects the diurnal rhythms of gut microbial composition and metabolism in a mouse model of depression BMC Microbiology Depression Gut microbiota Circadian rhythms Metabolism |
| title | Chronic restraint stress affects the diurnal rhythms of gut microbial composition and metabolism in a mouse model of depression |
| title_full | Chronic restraint stress affects the diurnal rhythms of gut microbial composition and metabolism in a mouse model of depression |
| title_fullStr | Chronic restraint stress affects the diurnal rhythms of gut microbial composition and metabolism in a mouse model of depression |
| title_full_unstemmed | Chronic restraint stress affects the diurnal rhythms of gut microbial composition and metabolism in a mouse model of depression |
| title_short | Chronic restraint stress affects the diurnal rhythms of gut microbial composition and metabolism in a mouse model of depression |
| title_sort | chronic restraint stress affects the diurnal rhythms of gut microbial composition and metabolism in a mouse model of depression |
| topic | Depression Gut microbiota Circadian rhythms Metabolism |
| url | https://doi.org/10.1186/s12866-025-03764-4 |
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