Single-cell analysis of Mycobacterium tuberculosis with diverse drug resistance using surface-enhanced Raman spectroscopy (SERS)
In this work, we investigated individual bacteria M. tuberculosis belonging to strains of the Beijing family with different drug sensitivity (sensitive, multi and extensive drug-resistant) by surface-enhanced Raman spectroscopy (SERS) in the fingerprint region. The latter is focused on the spectral...
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PeerJ Inc.
2025-01-01
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| author | Andrey Zyubin Anastasia Lavrova Marine Dogonadze Evgenii Borisov Eugene B. Postnikov |
| author_facet | Andrey Zyubin Anastasia Lavrova Marine Dogonadze Evgenii Borisov Eugene B. Postnikov |
| author_sort | Andrey Zyubin |
| collection | DOAJ |
| description | In this work, we investigated individual bacteria M. tuberculosis belonging to strains of the Beijing family with different drug sensitivity (sensitive, multi and extensive drug-resistant) by surface-enhanced Raman spectroscopy (SERS) in the fingerprint region. The latter is focused on the spectral bands, which correspond to a set of glutathione bands and DNA methylation patterns revealed due to 5-methylcytosine spectral biomarkers. It is shown that these spectral features can be correlated with drug sensitivity and DNA methylation. Thus, since this kind of diagnostics is fast and operates with individual cells, it can be considered a promising tool, which significantly shortens the time required for a strain’s type identification necessary to prescribe adequate therapy. |
| format | Article |
| id | doaj-art-5fcda5f159af49ebbe5381598b0cbee4 |
| institution | Kabale University |
| issn | 2167-8359 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj-art-5fcda5f159af49ebbe5381598b0cbee42025-01-26T15:05:07ZengPeerJ Inc.PeerJ2167-83592025-01-0113e1883010.7717/peerj.18830Single-cell analysis of Mycobacterium tuberculosis with diverse drug resistance using surface-enhanced Raman spectroscopy (SERS)Andrey Zyubin0Anastasia Lavrova1Marine Dogonadze2Evgenii Borisov3Eugene B. Postnikov4Immanuel Kant Baltic Federal University, Kaliningrad, RussiaSaint-Petersburg State Research Institute of Phthisiopulmonology, Saint-Petersburg, RussiaSaint-Petersburg State Research Institute of Phthisiopulmonology, Saint-Petersburg, RussiaSaint-Petersburg State University, Saint-Petersburg, RussiaKursk State University, Kursk, RussiaIn this work, we investigated individual bacteria M. tuberculosis belonging to strains of the Beijing family with different drug sensitivity (sensitive, multi and extensive drug-resistant) by surface-enhanced Raman spectroscopy (SERS) in the fingerprint region. The latter is focused on the spectral bands, which correspond to a set of glutathione bands and DNA methylation patterns revealed due to 5-methylcytosine spectral biomarkers. It is shown that these spectral features can be correlated with drug sensitivity and DNA methylation. Thus, since this kind of diagnostics is fast and operates with individual cells, it can be considered a promising tool, which significantly shortens the time required for a strain’s type identification necessary to prescribe adequate therapy.https://peerj.com/articles/18830.pdfRaman spectroscopyTuberculosisMycobacterium tuberculosis |
| spellingShingle | Andrey Zyubin Anastasia Lavrova Marine Dogonadze Evgenii Borisov Eugene B. Postnikov Single-cell analysis of Mycobacterium tuberculosis with diverse drug resistance using surface-enhanced Raman spectroscopy (SERS) PeerJ Raman spectroscopy Tuberculosis Mycobacterium tuberculosis |
| title | Single-cell analysis of Mycobacterium tuberculosis with diverse drug resistance using surface-enhanced Raman spectroscopy (SERS) |
| title_full | Single-cell analysis of Mycobacterium tuberculosis with diverse drug resistance using surface-enhanced Raman spectroscopy (SERS) |
| title_fullStr | Single-cell analysis of Mycobacterium tuberculosis with diverse drug resistance using surface-enhanced Raman spectroscopy (SERS) |
| title_full_unstemmed | Single-cell analysis of Mycobacterium tuberculosis with diverse drug resistance using surface-enhanced Raman spectroscopy (SERS) |
| title_short | Single-cell analysis of Mycobacterium tuberculosis with diverse drug resistance using surface-enhanced Raman spectroscopy (SERS) |
| title_sort | single cell analysis of mycobacterium tuberculosis with diverse drug resistance using surface enhanced raman spectroscopy sers |
| topic | Raman spectroscopy Tuberculosis Mycobacterium tuberculosis |
| url | https://peerj.com/articles/18830.pdf |
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