Effects of Macleaya cordata extract supplementation in low-protein, low-fishmeal diets on growth, hematology, intestinal histology, and microbiota in largemouth bass (Micropterus salmoides)
The present study investigated the effects of supplementing Macleaya cordata extract (MCE) in low-fishmeal or low-protein diet on the growth performance, blood biochemical indices, intestinal morphology, and intestinal microbiota of juvenile largemouth bass. Two control diets were prepared: a low-pr...
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Elsevier
2025-03-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513425000043 |
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| author | Wenyuan Xu Ruijun Zhu Dianyu Huan Xiaoqin Li Xiangjun Leng |
| author_facet | Wenyuan Xu Ruijun Zhu Dianyu Huan Xiaoqin Li Xiangjun Leng |
| author_sort | Wenyuan Xu |
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| description | The present study investigated the effects of supplementing Macleaya cordata extract (MCE) in low-fishmeal or low-protein diet on the growth performance, blood biochemical indices, intestinal morphology, and intestinal microbiota of juvenile largemouth bass. Two control diets were prepared: a low-protein diet (LP-0) containing 360 g/kg fishmeal and 460 g/kg crude protein, and a low-fishmeal diet (LF-0) containing 270 g/kg fishmeal and 500 g/kg crude protein. Then, 500 mg/kg MCE (Sangrovit) was added to the above two diets resulting in LP-500 and LF-500 diets, respectively. Juvenile largemouth bass (initial weight 21.0 ± 1.0 g) were fed the four diets for 60 days. Results showed that supplementing 500 mg/kg MCE in the low-protein diet, but not in the low-fishmeal diet, significantly increased the weight gain of largemouth bass (P < 0.05). In Aeromonashydrophila challenge test, the LP-500 group showed higher survival than the LP-0 group.In serum indices, LP-500 group presented significantly lower activity of diamine oxidase than the LP-0 group(P < 0.05). Supplementing MCE in low-fishmeal or low-protein diets significantly increased the abundance of Firmicutes in the intestinal microbiota (P < 0.05). Additionally, the expression levels of the inflammatory factors IL-10 and TGF-β1 were reduced. In summary, supplementing MCE in low-protein diet significantly improved the weight gain (P < 0.05), and MCE supplementation in low-protein or low-fishmeal diet could modulate the intestinal microbiota composition and reduce the inflammation factors expression in largemouth bass. |
| format | Article |
| id | doaj-art-5fb6383aea5f43e19d9b916fc2499f04 |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-5fb6383aea5f43e19d9b916fc2499f042025-02-06T05:12:19ZengElsevierAquaculture Reports2352-51342025-03-0140102618Effects of Macleaya cordata extract supplementation in low-protein, low-fishmeal diets on growth, hematology, intestinal histology, and microbiota in largemouth bass (Micropterus salmoides)Wenyuan Xu0Ruijun Zhu1Dianyu Huan2Xiaoqin Li3Xiangjun Leng4National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; Centre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai 201306, ChinaNanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Organic Food Development and Certification Center of China, Nanjing 210042, China; Organic Food Development and Certification Center of China, Nanjing 210042, ChinaPhytobiotics(Jiangsu)Biotech Co.,LTD., Changzou 213200, ChinaNational Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; Centre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai 201306, China; Correspondence to: Hucheng Ring road 999, Lingang New City, Shanghai 201306, China.National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; Centre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai 201306, China; Correspondence to: Hucheng Ring road 999, Lingang New City, Shanghai 201306, China.The present study investigated the effects of supplementing Macleaya cordata extract (MCE) in low-fishmeal or low-protein diet on the growth performance, blood biochemical indices, intestinal morphology, and intestinal microbiota of juvenile largemouth bass. Two control diets were prepared: a low-protein diet (LP-0) containing 360 g/kg fishmeal and 460 g/kg crude protein, and a low-fishmeal diet (LF-0) containing 270 g/kg fishmeal and 500 g/kg crude protein. Then, 500 mg/kg MCE (Sangrovit) was added to the above two diets resulting in LP-500 and LF-500 diets, respectively. Juvenile largemouth bass (initial weight 21.0 ± 1.0 g) were fed the four diets for 60 days. Results showed that supplementing 500 mg/kg MCE in the low-protein diet, but not in the low-fishmeal diet, significantly increased the weight gain of largemouth bass (P < 0.05). In Aeromonashydrophila challenge test, the LP-500 group showed higher survival than the LP-0 group.In serum indices, LP-500 group presented significantly lower activity of diamine oxidase than the LP-0 group(P < 0.05). Supplementing MCE in low-fishmeal or low-protein diets significantly increased the abundance of Firmicutes in the intestinal microbiota (P < 0.05). Additionally, the expression levels of the inflammatory factors IL-10 and TGF-β1 were reduced. In summary, supplementing MCE in low-protein diet significantly improved the weight gain (P < 0.05), and MCE supplementation in low-protein or low-fishmeal diet could modulate the intestinal microbiota composition and reduce the inflammation factors expression in largemouth bass.http://www.sciencedirect.com/science/article/pii/S2352513425000043Macleaya cordata extractLargemouth bassGrowthInflammation factorsIntestinal microbiota |
| spellingShingle | Wenyuan Xu Ruijun Zhu Dianyu Huan Xiaoqin Li Xiangjun Leng Effects of Macleaya cordata extract supplementation in low-protein, low-fishmeal diets on growth, hematology, intestinal histology, and microbiota in largemouth bass (Micropterus salmoides) Aquaculture Reports Macleaya cordata extract Largemouth bass Growth Inflammation factors Intestinal microbiota |
| title | Effects of Macleaya cordata extract supplementation in low-protein, low-fishmeal diets on growth, hematology, intestinal histology, and microbiota in largemouth bass (Micropterus salmoides) |
| title_full | Effects of Macleaya cordata extract supplementation in low-protein, low-fishmeal diets on growth, hematology, intestinal histology, and microbiota in largemouth bass (Micropterus salmoides) |
| title_fullStr | Effects of Macleaya cordata extract supplementation in low-protein, low-fishmeal diets on growth, hematology, intestinal histology, and microbiota in largemouth bass (Micropterus salmoides) |
| title_full_unstemmed | Effects of Macleaya cordata extract supplementation in low-protein, low-fishmeal diets on growth, hematology, intestinal histology, and microbiota in largemouth bass (Micropterus salmoides) |
| title_short | Effects of Macleaya cordata extract supplementation in low-protein, low-fishmeal diets on growth, hematology, intestinal histology, and microbiota in largemouth bass (Micropterus salmoides) |
| title_sort | effects of macleaya cordata extract supplementation in low protein low fishmeal diets on growth hematology intestinal histology and microbiota in largemouth bass micropterus salmoides |
| topic | Macleaya cordata extract Largemouth bass Growth Inflammation factors Intestinal microbiota |
| url | http://www.sciencedirect.com/science/article/pii/S2352513425000043 |
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