Application of Three-Dimensional Imaging to the Intestinal Crypt Organoids and Biopsied Intestinal Tissues
Two-dimensional (2D) histopathology is the standard analytical method for intestinal biopsied tissues; however, the role of 3-dimensional (3D) imaging system in the analysis of the intestinal tissues is unclear. The 3D structure of the crypt organoids from the intestinal stem cell culture and intest...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/624342 |
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| author | Yun Chen Ya-Hui Tsai Yuan-An Liu Shih-Hua Lee Sheng-Hong Tseng Shiue-Cheng Tang |
| author_facet | Yun Chen Ya-Hui Tsai Yuan-An Liu Shih-Hua Lee Sheng-Hong Tseng Shiue-Cheng Tang |
| author_sort | Yun Chen |
| collection | DOAJ |
| description | Two-dimensional (2D) histopathology is the standard analytical method for intestinal biopsied tissues; however, the role of 3-dimensional (3D) imaging system in the analysis of the intestinal tissues is unclear. The 3D structure of the crypt organoids from the intestinal stem cell culture and intestinal tissues from the donors and recipients after intestinal transplantation was observed using a 3D imaging system and compared with 2D histopathology and immunohistochemistry. The crypt organoids and intestinal tissues showed well-defined 3D structures. The 3D images of the intestinal tissues with acute rejection revealed absence of villi and few crypts, which were consistent with the histopathological features. In the intestinal transplant for megacystis microcolon intestinal hypoperistalsis syndrome, the donor’s intestinal tissues had well-developed nerve networks and interstitial cells of Cajal (ICCs) in the muscle layer, while the recipient’s intestinal tissues had distorted nerve network and the ICCs were few and sparsely distributed, relative to those of the donor. The 3D images showed a clear spatial relationship between the microstructures of the small bowel and the features of graft rejection. In conclusion, integration of the 3D imaging and 2D histopathology provided a global view of the intestinal tissues from the transplant patients. |
| format | Article |
| id | doaj-art-5f93c058aca845a3b00456158d745cfd |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-5f93c058aca845a3b00456158d745cfd2025-02-03T01:31:30ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/624342624342Application of Three-Dimensional Imaging to the Intestinal Crypt Organoids and Biopsied Intestinal TissuesYun Chen0Ya-Hui Tsai1Yuan-An Liu2Shih-Hua Lee3Sheng-Hong Tseng4Shiue-Cheng Tang5Department of Surgery, Far Eastern Memorial Hospital, Pan-Chiao, New Taipei 220, TaiwanDepartment of Surgery, Far Eastern Memorial Hospital, Pan-Chiao, New Taipei 220, TaiwanDepartment of Chemical Engineering, National Tsing Hua University, Hsinchu 300, TaiwanDepartment of Surgery, Far Eastern Memorial Hospital, Pan-Chiao, New Taipei 220, TaiwanDepartment of Surgery, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei 100, TaiwanDepartment of Chemical Engineering, National Tsing Hua University, Hsinchu 300, TaiwanTwo-dimensional (2D) histopathology is the standard analytical method for intestinal biopsied tissues; however, the role of 3-dimensional (3D) imaging system in the analysis of the intestinal tissues is unclear. The 3D structure of the crypt organoids from the intestinal stem cell culture and intestinal tissues from the donors and recipients after intestinal transplantation was observed using a 3D imaging system and compared with 2D histopathology and immunohistochemistry. The crypt organoids and intestinal tissues showed well-defined 3D structures. The 3D images of the intestinal tissues with acute rejection revealed absence of villi and few crypts, which were consistent with the histopathological features. In the intestinal transplant for megacystis microcolon intestinal hypoperistalsis syndrome, the donor’s intestinal tissues had well-developed nerve networks and interstitial cells of Cajal (ICCs) in the muscle layer, while the recipient’s intestinal tissues had distorted nerve network and the ICCs were few and sparsely distributed, relative to those of the donor. The 3D images showed a clear spatial relationship between the microstructures of the small bowel and the features of graft rejection. In conclusion, integration of the 3D imaging and 2D histopathology provided a global view of the intestinal tissues from the transplant patients.http://dx.doi.org/10.1155/2013/624342 |
| spellingShingle | Yun Chen Ya-Hui Tsai Yuan-An Liu Shih-Hua Lee Sheng-Hong Tseng Shiue-Cheng Tang Application of Three-Dimensional Imaging to the Intestinal Crypt Organoids and Biopsied Intestinal Tissues The Scientific World Journal |
| title | Application of Three-Dimensional Imaging to the Intestinal Crypt Organoids and Biopsied Intestinal Tissues |
| title_full | Application of Three-Dimensional Imaging to the Intestinal Crypt Organoids and Biopsied Intestinal Tissues |
| title_fullStr | Application of Three-Dimensional Imaging to the Intestinal Crypt Organoids and Biopsied Intestinal Tissues |
| title_full_unstemmed | Application of Three-Dimensional Imaging to the Intestinal Crypt Organoids and Biopsied Intestinal Tissues |
| title_short | Application of Three-Dimensional Imaging to the Intestinal Crypt Organoids and Biopsied Intestinal Tissues |
| title_sort | application of three dimensional imaging to the intestinal crypt organoids and biopsied intestinal tissues |
| url | http://dx.doi.org/10.1155/2013/624342 |
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