Ex Vivo Antioxidant Activity of Selected Medicinal Plants against Fenton Reaction-Mediated Oxidation of Biological Lipid Substrates
Free radical-mediated oxidation is often linked to various degenerative diseases. Biological substrates with lipids as major components are susceptible to oxygen-derived lipid peroxidation due to their composition. Lipid peroxide products act as biomarkers in evaluating the antioxidant potential of...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
|
| Series: | Biochemistry Research International |
| Online Access: | http://dx.doi.org/10.1155/2015/728621 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832560872187232256 |
|---|---|
| author | Namratha Pai Kotebagilu Vanitha Reddy Palvai Asna Urooj |
| author_facet | Namratha Pai Kotebagilu Vanitha Reddy Palvai Asna Urooj |
| author_sort | Namratha Pai Kotebagilu |
| collection | DOAJ |
| description | Free radical-mediated oxidation is often linked to various degenerative diseases. Biological substrates with lipids as major components are susceptible to oxygen-derived lipid peroxidation due to their composition. Lipid peroxide products act as biomarkers in evaluating the antioxidant potential of various plants and functional foods. The study focused on evaluation of the antioxidant potential of two extracts (methanol and 80% methanol) of four medicinal plants, Andrographis paniculata, Costus speciosus, Canthium parviflorum, and Abrus precatorius, against Fenton reaction-mediated oxidation of three biological lipid substrates; cholesterol, low-density lipoprotein, and brain homogenate. The antioxidant activity of the extracts was measured by thiobarbituric acid reactive substances method. Also, the correlation between the polyphenol, flavonoid content, and the antioxidant activity in biological substrates was analyzed. Results indicated highest antioxidant potential by 80% methanol extract of Canthium parviflorum (97.55%), methanol extract of Andrographis paniculata (72.15%), and methanol extract of Canthium parviflorum (49.55%) in cholesterol, low-density lipoprotein, and brain, respectively. The polyphenol and flavonoid contents of methanol extract of Andrographis paniculata in cholesterol (r=0.816) and low-density lipoprotein (r=0.948) and Costus speciosus in brain (r=0.977, polyphenols, and r=0.949, flavonoids) correlated well with the antioxidant activity. The findings prove the antioxidant potential of the selected medicinal plants against Fenton reaction in biological lipid substrates. |
| format | Article |
| id | doaj-art-5f8f7dc2696e4bc298eb0b9529f4047f |
| institution | Kabale University |
| issn | 2090-2247 2090-2255 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Biochemistry Research International |
| spelling | doaj-art-5f8f7dc2696e4bc298eb0b9529f4047f2025-02-03T01:26:28ZengWileyBiochemistry Research International2090-22472090-22552015-01-01201510.1155/2015/728621728621Ex Vivo Antioxidant Activity of Selected Medicinal Plants against Fenton Reaction-Mediated Oxidation of Biological Lipid SubstratesNamratha Pai Kotebagilu0Vanitha Reddy Palvai1Asna Urooj2Department of Studies in Food Science and Nutrition, University of Mysore, Manasagangothri, Mysuru, Karnataka 570006, IndiaDepartment of Studies in Food Science and Nutrition, University of Mysore, Manasagangothri, Mysuru, Karnataka 570006, IndiaDepartment of Studies in Food Science and Nutrition, University of Mysore, Manasagangothri, Mysuru, Karnataka 570006, IndiaFree radical-mediated oxidation is often linked to various degenerative diseases. Biological substrates with lipids as major components are susceptible to oxygen-derived lipid peroxidation due to their composition. Lipid peroxide products act as biomarkers in evaluating the antioxidant potential of various plants and functional foods. The study focused on evaluation of the antioxidant potential of two extracts (methanol and 80% methanol) of four medicinal plants, Andrographis paniculata, Costus speciosus, Canthium parviflorum, and Abrus precatorius, against Fenton reaction-mediated oxidation of three biological lipid substrates; cholesterol, low-density lipoprotein, and brain homogenate. The antioxidant activity of the extracts was measured by thiobarbituric acid reactive substances method. Also, the correlation between the polyphenol, flavonoid content, and the antioxidant activity in biological substrates was analyzed. Results indicated highest antioxidant potential by 80% methanol extract of Canthium parviflorum (97.55%), methanol extract of Andrographis paniculata (72.15%), and methanol extract of Canthium parviflorum (49.55%) in cholesterol, low-density lipoprotein, and brain, respectively. The polyphenol and flavonoid contents of methanol extract of Andrographis paniculata in cholesterol (r=0.816) and low-density lipoprotein (r=0.948) and Costus speciosus in brain (r=0.977, polyphenols, and r=0.949, flavonoids) correlated well with the antioxidant activity. The findings prove the antioxidant potential of the selected medicinal plants against Fenton reaction in biological lipid substrates.http://dx.doi.org/10.1155/2015/728621 |
| spellingShingle | Namratha Pai Kotebagilu Vanitha Reddy Palvai Asna Urooj Ex Vivo Antioxidant Activity of Selected Medicinal Plants against Fenton Reaction-Mediated Oxidation of Biological Lipid Substrates Biochemistry Research International |
| title | Ex Vivo Antioxidant Activity of Selected Medicinal Plants against Fenton Reaction-Mediated Oxidation of Biological Lipid Substrates |
| title_full | Ex Vivo Antioxidant Activity of Selected Medicinal Plants against Fenton Reaction-Mediated Oxidation of Biological Lipid Substrates |
| title_fullStr | Ex Vivo Antioxidant Activity of Selected Medicinal Plants against Fenton Reaction-Mediated Oxidation of Biological Lipid Substrates |
| title_full_unstemmed | Ex Vivo Antioxidant Activity of Selected Medicinal Plants against Fenton Reaction-Mediated Oxidation of Biological Lipid Substrates |
| title_short | Ex Vivo Antioxidant Activity of Selected Medicinal Plants against Fenton Reaction-Mediated Oxidation of Biological Lipid Substrates |
| title_sort | ex vivo antioxidant activity of selected medicinal plants against fenton reaction mediated oxidation of biological lipid substrates |
| url | http://dx.doi.org/10.1155/2015/728621 |
| work_keys_str_mv | AT namrathapaikotebagilu exvivoantioxidantactivityofselectedmedicinalplantsagainstfentonreactionmediatedoxidationofbiologicallipidsubstrates AT vanithareddypalvai exvivoantioxidantactivityofselectedmedicinalplantsagainstfentonreactionmediatedoxidationofbiologicallipidsubstrates AT asnaurooj exvivoantioxidantactivityofselectedmedicinalplantsagainstfentonreactionmediatedoxidationofbiologicallipidsubstrates |