Egyptian Novel Goose Parvovirus in Immune Organs of Naturally Infected Ducks: Next-Generation Sequencing, Immunohistochemical Signals, and Comparative Analysis of Pathological Changes Using Multiple Correspondence and Hierarchical Clustering Approach
The present study aims to better understand the nature of currently circulating GPV strains and their pathological impact on the immune system during natural outbreaks among different duck breeds in Egypt. For this purpose, 99 ducks (25 flocks) of different breeds, aged 14–75 days, were clinically e...
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| author | Mohamed A. Lebdah Amal A. M. Eid Reham M. ElBakrey Abd Elgalil. El-Gohary Mohamed G. Seadawy Mohamed R. Mousa Hagar F. Gouda Nehal I. A. Goda Mostafa F. El-Hosseny Ahmed S. El-tahlawy Rokayya Sami Rasha A. Al-Eisa Sarah S. Helal |
| author_facet | Mohamed A. Lebdah Amal A. M. Eid Reham M. ElBakrey Abd Elgalil. El-Gohary Mohamed G. Seadawy Mohamed R. Mousa Hagar F. Gouda Nehal I. A. Goda Mostafa F. El-Hosseny Ahmed S. El-tahlawy Rokayya Sami Rasha A. Al-Eisa Sarah S. Helal |
| author_sort | Mohamed A. Lebdah |
| collection | DOAJ |
| description | The present study aims to better understand the nature of currently circulating GPV strains and their pathological impact on the immune system during natural outbreaks among different duck breeds in Egypt. For this purpose, 99 ducks (25 flocks) of different breeds, aged 14–75 days, were clinically examined, and 75 tissue pools from the thymus, bursa of Fabricius, and spleen were submitted for virus detection and identification. Clinical and postmortem findings were suggestive of GPV infection. Concerning the immune system organs, atrophy in the thymus (60.6%), bursa (45.5%), and spleen (38.3%) was the most common gross lesion. Microscopically, the pathological impact of the virus was exhibited by a necrotic thymic cortex with Hassall’s corpuscle disintegration, the disappearance of normal bursal histological morphology accompanied by atrophied follicles and lymphocytic depletion, and apoptosis of B-lymphocytes in lymphoid follicles of the spleen. Furthermore, immunohistochemical examination revealed positive signals of the parvovirus detected in thymic lymphocytes in the cortex, bursa-dependent lymphoid follicle of the medulla, and diffuse positive expression of viral antigens in the spleen. GPV was detected in ducks using polymerase chain reaction, with the highest percentage of positive detection in the bursa of Fabricius (76%). Next-generation sequencing and phylogenetic analysis revealed that the detected virus was a variant of GPV, globally named novel GPV (NGPV), and closely related to Chinese NGPV isolates. To our knowledge, the current study is pioneering to address the immunopathological impact of NGPV among naturally infected ducks confirmed with full genome sequencing and immunohistochemical identification worldwide. |
| format | Article |
| id | doaj-art-5f8b14f42bd646a998baf292a7b6420a |
| institution | Kabale University |
| issn | 1999-4915 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj-art-5f8b14f42bd646a998baf292a7b6420a2025-01-24T13:52:35ZengMDPI AGViruses1999-49152025-01-011719610.3390/v17010096Egyptian Novel Goose Parvovirus in Immune Organs of Naturally Infected Ducks: Next-Generation Sequencing, Immunohistochemical Signals, and Comparative Analysis of Pathological Changes Using Multiple Correspondence and Hierarchical Clustering ApproachMohamed A. Lebdah0Amal A. M. Eid1Reham M. ElBakrey2Abd Elgalil. El-Gohary3Mohamed G. Seadawy4Mohamed R. Mousa5Hagar F. Gouda6Nehal I. A. Goda7Mostafa F. El-Hosseny8Ahmed S. El-tahlawy9Rokayya Sami10Rasha A. Al-Eisa11Sarah S. Helal12Department of Avian and Rabbit Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, EgyptDepartment of Avian and Rabbit Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, EgyptDepartment of Avian and Rabbit Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, EgyptDepartment of Poultry and Rabbit Diseases, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33511, EgyptBiodefense Center for Infectious and Emerging Diseases, Ministry of Defense, Cairo 11775, EgyptDepartment of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, EgyptDepartment of Animal Wealth Development (Biostatistics Subdivision), Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, EgyptDepartment of Histology and Cytology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, EgyptBiodefense Center for Infectious and Emerging Diseases, Ministry of Defense, Cairo 11775, EgyptFood Hygiene, Safety, and Technology Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, EgyptDepartment of Food Science and Nutrition, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Biology, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Avian and Rabbit Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, EgyptThe present study aims to better understand the nature of currently circulating GPV strains and their pathological impact on the immune system during natural outbreaks among different duck breeds in Egypt. For this purpose, 99 ducks (25 flocks) of different breeds, aged 14–75 days, were clinically examined, and 75 tissue pools from the thymus, bursa of Fabricius, and spleen were submitted for virus detection and identification. Clinical and postmortem findings were suggestive of GPV infection. Concerning the immune system organs, atrophy in the thymus (60.6%), bursa (45.5%), and spleen (38.3%) was the most common gross lesion. Microscopically, the pathological impact of the virus was exhibited by a necrotic thymic cortex with Hassall’s corpuscle disintegration, the disappearance of normal bursal histological morphology accompanied by atrophied follicles and lymphocytic depletion, and apoptosis of B-lymphocytes in lymphoid follicles of the spleen. Furthermore, immunohistochemical examination revealed positive signals of the parvovirus detected in thymic lymphocytes in the cortex, bursa-dependent lymphoid follicle of the medulla, and diffuse positive expression of viral antigens in the spleen. GPV was detected in ducks using polymerase chain reaction, with the highest percentage of positive detection in the bursa of Fabricius (76%). Next-generation sequencing and phylogenetic analysis revealed that the detected virus was a variant of GPV, globally named novel GPV (NGPV), and closely related to Chinese NGPV isolates. To our knowledge, the current study is pioneering to address the immunopathological impact of NGPV among naturally infected ducks confirmed with full genome sequencing and immunohistochemical identification worldwide.https://www.mdpi.com/1999-4915/17/1/96novel goose parvovirusimmunohistochemistrymultiple correspondencehierarchical clusteringnext-generation sequencingEgypt |
| spellingShingle | Mohamed A. Lebdah Amal A. M. Eid Reham M. ElBakrey Abd Elgalil. El-Gohary Mohamed G. Seadawy Mohamed R. Mousa Hagar F. Gouda Nehal I. A. Goda Mostafa F. El-Hosseny Ahmed S. El-tahlawy Rokayya Sami Rasha A. Al-Eisa Sarah S. Helal Egyptian Novel Goose Parvovirus in Immune Organs of Naturally Infected Ducks: Next-Generation Sequencing, Immunohistochemical Signals, and Comparative Analysis of Pathological Changes Using Multiple Correspondence and Hierarchical Clustering Approach Viruses novel goose parvovirus immunohistochemistry multiple correspondence hierarchical clustering next-generation sequencing Egypt |
| title | Egyptian Novel Goose Parvovirus in Immune Organs of Naturally Infected Ducks: Next-Generation Sequencing, Immunohistochemical Signals, and Comparative Analysis of Pathological Changes Using Multiple Correspondence and Hierarchical Clustering Approach |
| title_full | Egyptian Novel Goose Parvovirus in Immune Organs of Naturally Infected Ducks: Next-Generation Sequencing, Immunohistochemical Signals, and Comparative Analysis of Pathological Changes Using Multiple Correspondence and Hierarchical Clustering Approach |
| title_fullStr | Egyptian Novel Goose Parvovirus in Immune Organs of Naturally Infected Ducks: Next-Generation Sequencing, Immunohistochemical Signals, and Comparative Analysis of Pathological Changes Using Multiple Correspondence and Hierarchical Clustering Approach |
| title_full_unstemmed | Egyptian Novel Goose Parvovirus in Immune Organs of Naturally Infected Ducks: Next-Generation Sequencing, Immunohistochemical Signals, and Comparative Analysis of Pathological Changes Using Multiple Correspondence and Hierarchical Clustering Approach |
| title_short | Egyptian Novel Goose Parvovirus in Immune Organs of Naturally Infected Ducks: Next-Generation Sequencing, Immunohistochemical Signals, and Comparative Analysis of Pathological Changes Using Multiple Correspondence and Hierarchical Clustering Approach |
| title_sort | egyptian novel goose parvovirus in immune organs of naturally infected ducks next generation sequencing immunohistochemical signals and comparative analysis of pathological changes using multiple correspondence and hierarchical clustering approach |
| topic | novel goose parvovirus immunohistochemistry multiple correspondence hierarchical clustering next-generation sequencing Egypt |
| url | https://www.mdpi.com/1999-4915/17/1/96 |
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