Aggregation‐based dual‐target probe for dual‐colour super‐resolution monitoring mitophagy and evaluating drugs regulating mitochondria
Abstract Developing single fluorescent probe for simultaneously visualizing mitophagy flux and oxidative stress with super‐resolution is highly demanded yet quite challenging. Herein, a ratiometric HClO probe AHOH is designed and synthesized which is capable of simultaneously staining lysosomes and...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2025-01-01
|
| Series: | Aggregate |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/agt2.641 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832592351791415296 |
|---|---|
| author | Xiu‐Zhi Yang Hongbao Fang Shumeng Li Chengyan Chu Yunhua Zhang Ying Yang Weijiang He Yuncong Chen Zijian Guo |
| author_facet | Xiu‐Zhi Yang Hongbao Fang Shumeng Li Chengyan Chu Yunhua Zhang Ying Yang Weijiang He Yuncong Chen Zijian Guo |
| author_sort | Xiu‐Zhi Yang |
| collection | DOAJ |
| description | Abstract Developing single fluorescent probe for simultaneously visualizing mitophagy flux and oxidative stress with super‐resolution is highly demanded yet quite challenging. Herein, a ratiometric HClO probe AHOH is designed and synthesized which is capable of simultaneously staining lysosomes and mitochondria with red and green colour, respectively. AHOH could be selectivity oxidized by HClO, leading to a large emission blue shift (90 nm) and an over 1300‐fold enhancement of the emission ratio of Fl547nm/Fl637nm. We apply AHOH in super‐resolution microscopy and clearly visualize the dynamics of mitochondria–lysosomes interactions and the oxidative stress states upon different stimuli. Mitochondria dysfunction triggered by different drugs and genetic defect lead to elevated oxidative stress and higher levels of mitophagy. Moreover, AHOH could serve as a reliable tool for evaluating the efficacy of drugs regulating mitochondria dysfunction. This work provides a powerful dual‐colour super‐resolution imaging agent for real‐time monitoring the dynamics of organelle interactions and oxidative stress. |
| format | Article |
| id | doaj-art-5f7fe9924cc743c5b33f66f788c9f520 |
| institution | Kabale University |
| issn | 2692-4560 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Aggregate |
| spelling | doaj-art-5f7fe9924cc743c5b33f66f788c9f5202025-01-21T08:57:07ZengWileyAggregate2692-45602025-01-0161n/an/a10.1002/agt2.641Aggregation‐based dual‐target probe for dual‐colour super‐resolution monitoring mitophagy and evaluating drugs regulating mitochondriaXiu‐Zhi Yang0Hongbao Fang1Shumeng Li2Chengyan Chu3Yunhua Zhang4Ying Yang5Weijiang He6Yuncong Chen7Zijian Guo8State Key Laboratory of Coordination Chemistry School of Chemistry and Chemical Engineering Chemistry and Biomedicine Innovation Center (ChemBIC) Nanjing University Nanjing ChinaState Key Laboratory of Coordination Chemistry School of Chemistry and Chemical Engineering Chemistry and Biomedicine Innovation Center (ChemBIC) Nanjing University Nanjing ChinaState Key Laboratory of Coordination Chemistry School of Chemistry and Chemical Engineering Chemistry and Biomedicine Innovation Center (ChemBIC) Nanjing University Nanjing ChinaState Key Laboratory of Coordination Chemistry School of Chemistry and Chemical Engineering Chemistry and Biomedicine Innovation Center (ChemBIC) Nanjing University Nanjing ChinaState Key Laboratory of Coordination Chemistry School of Chemistry and Chemical Engineering Chemistry and Biomedicine Innovation Center (ChemBIC) Nanjing University Nanjing ChinaState Key Laboratory of Coordination Chemistry School of Chemistry and Chemical Engineering Chemistry and Biomedicine Innovation Center (ChemBIC) Nanjing University Nanjing ChinaState Key Laboratory of Coordination Chemistry School of Chemistry and Chemical Engineering Chemistry and Biomedicine Innovation Center (ChemBIC) Nanjing University Nanjing ChinaState Key Laboratory of Coordination Chemistry School of Chemistry and Chemical Engineering Chemistry and Biomedicine Innovation Center (ChemBIC) Nanjing University Nanjing ChinaState Key Laboratory of Coordination Chemistry School of Chemistry and Chemical Engineering Chemistry and Biomedicine Innovation Center (ChemBIC) Nanjing University Nanjing ChinaAbstract Developing single fluorescent probe for simultaneously visualizing mitophagy flux and oxidative stress with super‐resolution is highly demanded yet quite challenging. Herein, a ratiometric HClO probe AHOH is designed and synthesized which is capable of simultaneously staining lysosomes and mitochondria with red and green colour, respectively. AHOH could be selectivity oxidized by HClO, leading to a large emission blue shift (90 nm) and an over 1300‐fold enhancement of the emission ratio of Fl547nm/Fl637nm. We apply AHOH in super‐resolution microscopy and clearly visualize the dynamics of mitochondria–lysosomes interactions and the oxidative stress states upon different stimuli. Mitochondria dysfunction triggered by different drugs and genetic defect lead to elevated oxidative stress and higher levels of mitophagy. Moreover, AHOH could serve as a reliable tool for evaluating the efficacy of drugs regulating mitochondria dysfunction. This work provides a powerful dual‐colour super‐resolution imaging agent for real‐time monitoring the dynamics of organelle interactions and oxidative stress.https://doi.org/10.1002/agt2.641drug evaluationdual‐colour imagingmitochondria–lysosomes interactionsnano‐aggregatesratiometric HClO sensing |
| spellingShingle | Xiu‐Zhi Yang Hongbao Fang Shumeng Li Chengyan Chu Yunhua Zhang Ying Yang Weijiang He Yuncong Chen Zijian Guo Aggregation‐based dual‐target probe for dual‐colour super‐resolution monitoring mitophagy and evaluating drugs regulating mitochondria Aggregate drug evaluation dual‐colour imaging mitochondria–lysosomes interactions nano‐aggregates ratiometric HClO sensing |
| title | Aggregation‐based dual‐target probe for dual‐colour super‐resolution monitoring mitophagy and evaluating drugs regulating mitochondria |
| title_full | Aggregation‐based dual‐target probe for dual‐colour super‐resolution monitoring mitophagy and evaluating drugs regulating mitochondria |
| title_fullStr | Aggregation‐based dual‐target probe for dual‐colour super‐resolution monitoring mitophagy and evaluating drugs regulating mitochondria |
| title_full_unstemmed | Aggregation‐based dual‐target probe for dual‐colour super‐resolution monitoring mitophagy and evaluating drugs regulating mitochondria |
| title_short | Aggregation‐based dual‐target probe for dual‐colour super‐resolution monitoring mitophagy and evaluating drugs regulating mitochondria |
| title_sort | aggregation based dual target probe for dual colour super resolution monitoring mitophagy and evaluating drugs regulating mitochondria |
| topic | drug evaluation dual‐colour imaging mitochondria–lysosomes interactions nano‐aggregates ratiometric HClO sensing |
| url | https://doi.org/10.1002/agt2.641 |
| work_keys_str_mv | AT xiuzhiyang aggregationbaseddualtargetprobefordualcoloursuperresolutionmonitoringmitophagyandevaluatingdrugsregulatingmitochondria AT hongbaofang aggregationbaseddualtargetprobefordualcoloursuperresolutionmonitoringmitophagyandevaluatingdrugsregulatingmitochondria AT shumengli aggregationbaseddualtargetprobefordualcoloursuperresolutionmonitoringmitophagyandevaluatingdrugsregulatingmitochondria AT chengyanchu aggregationbaseddualtargetprobefordualcoloursuperresolutionmonitoringmitophagyandevaluatingdrugsregulatingmitochondria AT yunhuazhang aggregationbaseddualtargetprobefordualcoloursuperresolutionmonitoringmitophagyandevaluatingdrugsregulatingmitochondria AT yingyang aggregationbaseddualtargetprobefordualcoloursuperresolutionmonitoringmitophagyandevaluatingdrugsregulatingmitochondria AT weijianghe aggregationbaseddualtargetprobefordualcoloursuperresolutionmonitoringmitophagyandevaluatingdrugsregulatingmitochondria AT yuncongchen aggregationbaseddualtargetprobefordualcoloursuperresolutionmonitoringmitophagyandevaluatingdrugsregulatingmitochondria AT zijianguo aggregationbaseddualtargetprobefordualcoloursuperresolutionmonitoringmitophagyandevaluatingdrugsregulatingmitochondria |