Regulation of noradrenaline synthesis, uptake, and degradation in the left ventricle by fatty acid amide hydrolase (FAAH) inhibitor URB597 in the chronic unpredictable stress model of depression
Depression has been linked to the dysfunction of the autonomic nervous system, which may cause dysregulation of the cardiovascular system. One promising therapeutic strategy for treating different diseases is inhibiting the enzyme fatty acid amide hydrolase (FAAH), which increases the availability o...
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2024-01-01
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| Series: | Archives of Biological Sciences |
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| Online Access: | https://doiserbia.nb.rs/img/doi/0354-4664/2024/0354-46642400028F.pdf |
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| author | Ferizović Harisa Spasojević Nataša Janković Milica Stefanović Bojana Dronjak Slađana |
| author_facet | Ferizović Harisa Spasojević Nataša Janković Milica Stefanović Bojana Dronjak Slađana |
| author_sort | Ferizović Harisa |
| collection | DOAJ |
| description | Depression has been linked to the dysfunction of the autonomic nervous system, which may cause dysregulation of the cardiovascular system. One promising therapeutic strategy for treating different diseases is inhibiting the enzyme fatty acid amide hydrolase (FAAH), which increases the availability of endogenous cannabinoids. We examined the effect of chronic FAAH inhibition with URB597 treatment on the noradrenaline (NA) content, synthesis, transport, and degradation in the left ventricle of female and male rats exposed to chronic unpredictable stress (CUS). CUS decreased the levels of both NA and dopamine-β-hydroxylase (DBH) protein in male rats and decreased NA transporter (NET) protein levels in female rats while elevating monoamine oxidase A (MAO-A) in both sexes. Intraperitoneal URB597 application led to increased expression of DBH in stressed males, as well as elevated NET protein levels and decreased MAO-A protein levels in the left ventricle of stressed rats of both sexes. URB597 treatment may have a beneficial effect on the cardiovascular system in an animal model of depression with heightened sympathoneural activity. |
| format | Article |
| id | doaj-art-5f567c9512b14cf6b256f61806584f30 |
| institution | Kabale University |
| issn | 0354-4664 1821-4339 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj-art-5f567c9512b14cf6b256f61806584f302025-01-30T06:38:34ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392024-01-0176335936710.2298/ABS240731028F0354-46642400028FRegulation of noradrenaline synthesis, uptake, and degradation in the left ventricle by fatty acid amide hydrolase (FAAH) inhibitor URB597 in the chronic unpredictable stress model of depressionFerizović Harisa0https://orcid.org/0000-0001-5667-7254Spasojević Nataša1https://orcid.org/0000-0001-8843-7976Janković Milica2https://orcid.org/0000-0001-5189-9472Stefanović Bojana3https://orcid.org/0000-0002-1360-5514Dronjak Slađana4https://orcid.org/0000-0002-4334-8002Department of Molecular Biology and Endocrinology, Institute of Nuclear Sciences “Vinča”, National Institute of the Republic of Serbia, University of Belgrade, Belgrade, SerbiaDepartment of Molecular Biology and Endocrinology, Institute of Nuclear Sciences “Vinča”, National Institute of the Republic of Serbia, University of Belgrade, Belgrade, SerbiaDepartment of Molecular Biology and Endocrinology, Institute of Nuclear Sciences “Vinča”, National Institute of the Republic of Serbia, University of Belgrade, Belgrade, SerbiaDepartment of Molecular Biology and Endocrinology, Institute of Nuclear Sciences “Vinča”, National Institute of the Republic of Serbia, University of Belgrade, Belgrade, SerbiaDepartment of Molecular Biology and Endocrinology, Institute of Nuclear Sciences “Vinča”, National Institute of the Republic of Serbia, University of Belgrade, Belgrade, SerbiaDepression has been linked to the dysfunction of the autonomic nervous system, which may cause dysregulation of the cardiovascular system. One promising therapeutic strategy for treating different diseases is inhibiting the enzyme fatty acid amide hydrolase (FAAH), which increases the availability of endogenous cannabinoids. We examined the effect of chronic FAAH inhibition with URB597 treatment on the noradrenaline (NA) content, synthesis, transport, and degradation in the left ventricle of female and male rats exposed to chronic unpredictable stress (CUS). CUS decreased the levels of both NA and dopamine-β-hydroxylase (DBH) protein in male rats and decreased NA transporter (NET) protein levels in female rats while elevating monoamine oxidase A (MAO-A) in both sexes. Intraperitoneal URB597 application led to increased expression of DBH in stressed males, as well as elevated NET protein levels and decreased MAO-A protein levels in the left ventricle of stressed rats of both sexes. URB597 treatment may have a beneficial effect on the cardiovascular system in an animal model of depression with heightened sympathoneural activity.https://doiserbia.nb.rs/img/doi/0354-4664/2024/0354-46642400028F.pdfnoradrenalineleft ventriclechronic unpredictable stressendocannabinoidsexperimental rat |
| spellingShingle | Ferizović Harisa Spasojević Nataša Janković Milica Stefanović Bojana Dronjak Slađana Regulation of noradrenaline synthesis, uptake, and degradation in the left ventricle by fatty acid amide hydrolase (FAAH) inhibitor URB597 in the chronic unpredictable stress model of depression Archives of Biological Sciences noradrenaline left ventricle chronic unpredictable stress endocannabinoids experimental rat |
| title | Regulation of noradrenaline synthesis, uptake, and degradation in the left ventricle by fatty acid amide hydrolase (FAAH) inhibitor URB597 in the chronic unpredictable stress model of depression |
| title_full | Regulation of noradrenaline synthesis, uptake, and degradation in the left ventricle by fatty acid amide hydrolase (FAAH) inhibitor URB597 in the chronic unpredictable stress model of depression |
| title_fullStr | Regulation of noradrenaline synthesis, uptake, and degradation in the left ventricle by fatty acid amide hydrolase (FAAH) inhibitor URB597 in the chronic unpredictable stress model of depression |
| title_full_unstemmed | Regulation of noradrenaline synthesis, uptake, and degradation in the left ventricle by fatty acid amide hydrolase (FAAH) inhibitor URB597 in the chronic unpredictable stress model of depression |
| title_short | Regulation of noradrenaline synthesis, uptake, and degradation in the left ventricle by fatty acid amide hydrolase (FAAH) inhibitor URB597 in the chronic unpredictable stress model of depression |
| title_sort | regulation of noradrenaline synthesis uptake and degradation in the left ventricle by fatty acid amide hydrolase faah inhibitor urb597 in the chronic unpredictable stress model of depression |
| topic | noradrenaline left ventricle chronic unpredictable stress endocannabinoids experimental rat |
| url | https://doiserbia.nb.rs/img/doi/0354-4664/2024/0354-46642400028F.pdf |
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