Comparative chemical investigation and evaluation of antioxidant and tyrosinase inhibitory effects of Withania somnifera (L.) Dunal and Solanum nigrum (L.) berries
In the present study, berries of two different species of Solanaceae family, Withania somnifera (WS) and Solanum nigrum (SN), were extracted in methanol and then fractionated with solvents, ranging from non-polar to polar, for their phytochemical profiling and investigation of antioxidant and tyrosi...
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2018-03-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.2478/acph-2018-0007 |
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| author | Hameed Abdul Akhtar Naveed |
| author_facet | Hameed Abdul Akhtar Naveed |
| author_sort | Hameed Abdul |
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| description | In the present study, berries of two different species of Solanaceae family, Withania somnifera (WS) and Solanum nigrum (SN), were extracted in methanol and then fractionated with solvents, ranging from non-polar to polar, for their phytochemical profiling and investigation of antioxidant and tyrosinase enzyme inhibition capacity. The methanolic extract and n-hexane, ethyl acetate (WSEA, SNEA) and aqueous fractions were chemically analyzed and evaluated for biological activity. Total flavonoids and total phenolics were quantified in WSEA (96.91 ± 1.56 μg QE mg-1 sample and 178.45 ± 2.78 μg GAE mg-1 s ample, r esp.) and S NEA (89.58 ± 0.98 μg QE mg-1 sample and 120.15 ± 2.33 μg GAE mg-1 sample, resp.). HPLC-DAD analysis of ethyl acetate fractions of WS and SN measured 13.74 and 5.34 μg GAE mg-1 dry fraction and 3.72 and 3.41 μg QE mg-1 dry fraction, resp. WSEA and SNEA fractions showed the highest 2,2-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging, total antioxidant capacity and iron reducing power activity. The highest inhibition of tyrosinase enzyme was also exhibited by WSEA and SNEA (59.6 and 58.7 %) resp. This investigation justifies the medicinal value of W. somnifera and S. nigrum berry extracts as potential and readily available sources of natural antioxidants. Marked tyrosinase enzyme inhibition activity and antioxidant activity of both plant extracts might be due to polyphenols and flavonoids. |
| format | Article |
| id | doaj-art-5ef36ce321e84152820a873dc2cf1778 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2018-03-01 |
| publisher | Sciendo |
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| series | Acta Pharmaceutica |
| spelling | doaj-art-5ef36ce321e84152820a873dc2cf17782025-02-02T15:49:11ZengSciendoActa Pharmaceutica1846-95582018-03-01681476010.2478/acph-2018-0007acph-2018-0007Comparative chemical investigation and evaluation of antioxidant and tyrosinase inhibitory effects of Withania somnifera (L.) Dunal and Solanum nigrum (L.) berriesHameed Abdul0Akhtar Naveed1Faculty of Pharmacy and Alternative Medicine The Islamia University of Bahawalpur 63100, PakistanFaculty of Pharmacy and Alternative Medicine The Islamia University of Bahawalpur 63100, PakistanIn the present study, berries of two different species of Solanaceae family, Withania somnifera (WS) and Solanum nigrum (SN), were extracted in methanol and then fractionated with solvents, ranging from non-polar to polar, for their phytochemical profiling and investigation of antioxidant and tyrosinase enzyme inhibition capacity. The methanolic extract and n-hexane, ethyl acetate (WSEA, SNEA) and aqueous fractions were chemically analyzed and evaluated for biological activity. Total flavonoids and total phenolics were quantified in WSEA (96.91 ± 1.56 μg QE mg-1 sample and 178.45 ± 2.78 μg GAE mg-1 s ample, r esp.) and S NEA (89.58 ± 0.98 μg QE mg-1 sample and 120.15 ± 2.33 μg GAE mg-1 sample, resp.). HPLC-DAD analysis of ethyl acetate fractions of WS and SN measured 13.74 and 5.34 μg GAE mg-1 dry fraction and 3.72 and 3.41 μg QE mg-1 dry fraction, resp. WSEA and SNEA fractions showed the highest 2,2-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging, total antioxidant capacity and iron reducing power activity. The highest inhibition of tyrosinase enzyme was also exhibited by WSEA and SNEA (59.6 and 58.7 %) resp. This investigation justifies the medicinal value of W. somnifera and S. nigrum berry extracts as potential and readily available sources of natural antioxidants. Marked tyrosinase enzyme inhibition activity and antioxidant activity of both plant extracts might be due to polyphenols and flavonoids.https://doi.org/10.2478/acph-2018-0007withania somniferasolanum nigrumpolyphenolsantioxidanttyrosinase inhibitionhplc-dad |
| spellingShingle | Hameed Abdul Akhtar Naveed Comparative chemical investigation and evaluation of antioxidant and tyrosinase inhibitory effects of Withania somnifera (L.) Dunal and Solanum nigrum (L.) berries Acta Pharmaceutica withania somnifera solanum nigrum polyphenols antioxidant tyrosinase inhibition hplc-dad |
| title | Comparative chemical investigation and evaluation of antioxidant and tyrosinase inhibitory effects of Withania somnifera (L.) Dunal and Solanum nigrum (L.) berries |
| title_full | Comparative chemical investigation and evaluation of antioxidant and tyrosinase inhibitory effects of Withania somnifera (L.) Dunal and Solanum nigrum (L.) berries |
| title_fullStr | Comparative chemical investigation and evaluation of antioxidant and tyrosinase inhibitory effects of Withania somnifera (L.) Dunal and Solanum nigrum (L.) berries |
| title_full_unstemmed | Comparative chemical investigation and evaluation of antioxidant and tyrosinase inhibitory effects of Withania somnifera (L.) Dunal and Solanum nigrum (L.) berries |
| title_short | Comparative chemical investigation and evaluation of antioxidant and tyrosinase inhibitory effects of Withania somnifera (L.) Dunal and Solanum nigrum (L.) berries |
| title_sort | comparative chemical investigation and evaluation of antioxidant and tyrosinase inhibitory effects of withania somnifera l dunal and solanum nigrum l berries |
| topic | withania somnifera solanum nigrum polyphenols antioxidant tyrosinase inhibition hplc-dad |
| url | https://doi.org/10.2478/acph-2018-0007 |
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