Exogenous LEA proteins expression enhances cold tolerance in mammalian cells by reducing oxidative stress
Abstract Understanding the molecular mechanisms that confer cold resistance in mammalian cells might be relevant for advancing medical applications. This study aimed to exploit the protective function of Late Embryogenesis Abundant (LEA) proteins, known to provide resistance to low temperatures in e...
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| Format: | Article |
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-86499-6 |
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| author | Martina Lo Sterzo Domenico Iuso Luca Palazzese Margherita Moncada Francesca Boffa Aurora Scudieri Luisa Gioia Marta Czernik Pasqualino Loi |
| author_facet | Martina Lo Sterzo Domenico Iuso Luca Palazzese Margherita Moncada Francesca Boffa Aurora Scudieri Luisa Gioia Marta Czernik Pasqualino Loi |
| author_sort | Martina Lo Sterzo |
| collection | DOAJ |
| description | Abstract Understanding the molecular mechanisms that confer cold resistance in mammalian cells might be relevant for advancing medical applications. This study aimed to exploit the protective function of Late Embryogenesis Abundant (LEA) proteins, known to provide resistance to low temperatures in extremophiles and plants, by their exogenous expression in mammalian cells, and compare their effects with the well characterized antioxidant, vitamin E. Remarkably, the expression of LEA proteins in mammalian cells exerted cold-protective effect similar to Vitamin E. LEA proteins preserved cell viability during cold stress and ensured a normal metabolic activity after warming. Their protective action was due to the mitigation of cold-induced mitochondrial stress and the overproduction of reactive oxygen species (ROS), leading in turn to enhanced cytoskeleton stability and decreased DNA damage. Our studies showed that plant-derived LEA proteins exhibit remarkable cold protection effects in mammalian cells through their potent antioxidant properties, which was found comparable to Vitamin E. |
| format | Article |
| id | doaj-art-5ef1b5be9c914c59af571a4677184e6a |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-5ef1b5be9c914c59af571a4677184e6a2025-02-02T12:24:21ZengNature PortfolioScientific Reports2045-23222025-01-0115111510.1038/s41598-025-86499-6Exogenous LEA proteins expression enhances cold tolerance in mammalian cells by reducing oxidative stressMartina Lo Sterzo0Domenico Iuso1Luca Palazzese2Margherita Moncada3Francesca Boffa4Aurora Scudieri5Luisa Gioia6Marta Czernik7Pasqualino Loi8Department of Veterinary Medicine, University of TeramoDepartment of Veterinary Medicine, University of TeramoDepartment of Veterinary Medicine, University of TeramoDepartment of Veterinary Medicine, University of TeramoDepartment of Veterinary Medicine, University of TeramoDepartment of Veterinary Medicine, University of TeramoDepartment of Bioscience and Technology for Food, Agriculture and Environment, University of TeramoDepartment of Veterinary Medicine, University of TeramoDepartment of Veterinary Medicine, University of TeramoAbstract Understanding the molecular mechanisms that confer cold resistance in mammalian cells might be relevant for advancing medical applications. This study aimed to exploit the protective function of Late Embryogenesis Abundant (LEA) proteins, known to provide resistance to low temperatures in extremophiles and plants, by their exogenous expression in mammalian cells, and compare their effects with the well characterized antioxidant, vitamin E. Remarkably, the expression of LEA proteins in mammalian cells exerted cold-protective effect similar to Vitamin E. LEA proteins preserved cell viability during cold stress and ensured a normal metabolic activity after warming. Their protective action was due to the mitigation of cold-induced mitochondrial stress and the overproduction of reactive oxygen species (ROS), leading in turn to enhanced cytoskeleton stability and decreased DNA damage. Our studies showed that plant-derived LEA proteins exhibit remarkable cold protection effects in mammalian cells through their potent antioxidant properties, which was found comparable to Vitamin E.https://doi.org/10.1038/s41598-025-86499-6Late Embryogenesis Abundant proteinsCellsCryoprotectionAntioxidants |
| spellingShingle | Martina Lo Sterzo Domenico Iuso Luca Palazzese Margherita Moncada Francesca Boffa Aurora Scudieri Luisa Gioia Marta Czernik Pasqualino Loi Exogenous LEA proteins expression enhances cold tolerance in mammalian cells by reducing oxidative stress Scientific Reports Late Embryogenesis Abundant proteins Cells Cryoprotection Antioxidants |
| title | Exogenous LEA proteins expression enhances cold tolerance in mammalian cells by reducing oxidative stress |
| title_full | Exogenous LEA proteins expression enhances cold tolerance in mammalian cells by reducing oxidative stress |
| title_fullStr | Exogenous LEA proteins expression enhances cold tolerance in mammalian cells by reducing oxidative stress |
| title_full_unstemmed | Exogenous LEA proteins expression enhances cold tolerance in mammalian cells by reducing oxidative stress |
| title_short | Exogenous LEA proteins expression enhances cold tolerance in mammalian cells by reducing oxidative stress |
| title_sort | exogenous lea proteins expression enhances cold tolerance in mammalian cells by reducing oxidative stress |
| topic | Late Embryogenesis Abundant proteins Cells Cryoprotection Antioxidants |
| url | https://doi.org/10.1038/s41598-025-86499-6 |
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