Wild pink bayberry free phenolic extract induces mitochondria-dependent apoptosis and G0/G1 cell cycle arrest through p38/MAPK and PI3K/Akt pathway in MDA-MB-231 cancer cells
Polyphenol-rich foods have been shown to be good for cancer prevention as powerful antioxidants. In this study, the mechanisms of wild pink bayberry free phenolic extract (WPBFE) inhibiting the proliferation and inducing apoptotic of MDA-MB-231 breast cancer cells was examined. The main phenolic aci...
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Tsinghua University Press
2023-09-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453023000149 |
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| author | Wen Xia Er-sheng Gong Yanyun Lin Bisheng Zheng Wenhan Yang Tong Li Sheng Zhang Peng Li Rui-hai Liu |
| author_facet | Wen Xia Er-sheng Gong Yanyun Lin Bisheng Zheng Wenhan Yang Tong Li Sheng Zhang Peng Li Rui-hai Liu |
| author_sort | Wen Xia |
| collection | DOAJ |
| description | Polyphenol-rich foods have been shown to be good for cancer prevention as powerful antioxidants. In this study, the mechanisms of wild pink bayberry free phenolic extract (WPBFE) inhibiting the proliferation and inducing apoptotic of MDA-MB-231 breast cancer cells was examined. The main phenolic acids and flavonols in WPBFE were gallic acid ((18.83 ± 0.44) μg/g FW) and myricetin ((1.52 ± 0.05) μg/g FW), respectively. The maximum inhibition rate of WPBFE at non-cytotoxicity dose (below 80 mg/mL) was 81 %. Western blotting analysis showed that WPBFE could cause the arrest of cell cycle in G0/G1 phase by down-regulating expression levels of PCNA, CDK4, cyclin D1 and up-regulating the expression level of p21. Meanwhile, WPBFE induced apoptosis through initiating the mitochondrial death pathway by up-regulating cleaved caspase-3 and enhancing the ratio of Bax/Bcl-2, with the maximum expression levels of 1.29 and 2.03 folds that of control group, respectively. Further study of the upstream protein, we found that WPBFE down-regulated TRAF2, while upregulated p-ASK1, p-p38 and p-p53. Furthermore, WPBFE could down-regulate the expression of p-PI3K and p-Akt. These observations indicated that WPBFE might play an anticancer role through regulating the p38 MAPK together with PI3K/Akt pathway. |
| format | Article |
| id | doaj-art-5edec3843c2e4a7c8625dd19846a8819 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-5edec3843c2e4a7c8625dd19846a88192025-02-03T00:27:37ZengTsinghua University PressFood Science and Human Wellness2213-45302023-09-0112515101518Wild pink bayberry free phenolic extract induces mitochondria-dependent apoptosis and G0/G1 cell cycle arrest through p38/MAPK and PI3K/Akt pathway in MDA-MB-231 cancer cellsWen Xia0Er-sheng Gong1Yanyun Lin2Bisheng Zheng3Wenhan Yang4Tong Li5Sheng Zhang6Peng Li7Rui-hai Liu8Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center), School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, ChinaSchool of Public Health and Health Management, Gannan Medical University, Ganzhou 341000, China; Corresponding author.Key Laboratory of Tropical Crop Products Processing of Ministry of Agriculture and Rural Affairs, Agricultural Products Processing Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524001, ChinaOverseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center), School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, ChinaOverseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center), School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, ChinaDepartment of Food Science, Cornell University, Ithaca 14853, USAOverseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center), School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, ChinaStem Cell Research and Cellular Therapy Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, ChinaDepartment of Food Science, Cornell University, Ithaca 14853, USA; Corresponding author.Polyphenol-rich foods have been shown to be good for cancer prevention as powerful antioxidants. In this study, the mechanisms of wild pink bayberry free phenolic extract (WPBFE) inhibiting the proliferation and inducing apoptotic of MDA-MB-231 breast cancer cells was examined. The main phenolic acids and flavonols in WPBFE were gallic acid ((18.83 ± 0.44) μg/g FW) and myricetin ((1.52 ± 0.05) μg/g FW), respectively. The maximum inhibition rate of WPBFE at non-cytotoxicity dose (below 80 mg/mL) was 81 %. Western blotting analysis showed that WPBFE could cause the arrest of cell cycle in G0/G1 phase by down-regulating expression levels of PCNA, CDK4, cyclin D1 and up-regulating the expression level of p21. Meanwhile, WPBFE induced apoptosis through initiating the mitochondrial death pathway by up-regulating cleaved caspase-3 and enhancing the ratio of Bax/Bcl-2, with the maximum expression levels of 1.29 and 2.03 folds that of control group, respectively. Further study of the upstream protein, we found that WPBFE down-regulated TRAF2, while upregulated p-ASK1, p-p38 and p-p53. Furthermore, WPBFE could down-regulate the expression of p-PI3K and p-Akt. These observations indicated that WPBFE might play an anticancer role through regulating the p38 MAPK together with PI3K/Akt pathway.http://www.sciencedirect.com/science/article/pii/S2213453023000149Phenolic extractBreast cancerCell cycleApoptosisCell proliferation |
| spellingShingle | Wen Xia Er-sheng Gong Yanyun Lin Bisheng Zheng Wenhan Yang Tong Li Sheng Zhang Peng Li Rui-hai Liu Wild pink bayberry free phenolic extract induces mitochondria-dependent apoptosis and G0/G1 cell cycle arrest through p38/MAPK and PI3K/Akt pathway in MDA-MB-231 cancer cells Food Science and Human Wellness Phenolic extract Breast cancer Cell cycle Apoptosis Cell proliferation |
| title | Wild pink bayberry free phenolic extract induces mitochondria-dependent apoptosis and G0/G1 cell cycle arrest through p38/MAPK and PI3K/Akt pathway in MDA-MB-231 cancer cells |
| title_full | Wild pink bayberry free phenolic extract induces mitochondria-dependent apoptosis and G0/G1 cell cycle arrest through p38/MAPK and PI3K/Akt pathway in MDA-MB-231 cancer cells |
| title_fullStr | Wild pink bayberry free phenolic extract induces mitochondria-dependent apoptosis and G0/G1 cell cycle arrest through p38/MAPK and PI3K/Akt pathway in MDA-MB-231 cancer cells |
| title_full_unstemmed | Wild pink bayberry free phenolic extract induces mitochondria-dependent apoptosis and G0/G1 cell cycle arrest through p38/MAPK and PI3K/Akt pathway in MDA-MB-231 cancer cells |
| title_short | Wild pink bayberry free phenolic extract induces mitochondria-dependent apoptosis and G0/G1 cell cycle arrest through p38/MAPK and PI3K/Akt pathway in MDA-MB-231 cancer cells |
| title_sort | wild pink bayberry free phenolic extract induces mitochondria dependent apoptosis and g0 g1 cell cycle arrest through p38 mapk and pi3k akt pathway in mda mb 231 cancer cells |
| topic | Phenolic extract Breast cancer Cell cycle Apoptosis Cell proliferation |
| url | http://www.sciencedirect.com/science/article/pii/S2213453023000149 |
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