A Triple-Precursor Blend as a Topical Solution to Protect the Skin Against Environmental Damage

The epidermis acts as the body’s primary defense, relying on components like lipids, HA and GSH for skin barrier function, hydration and resistance to oxidative stress. However, limitations in the topical application of these biomolecules call for novel approaches. This study investigates the effica...

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Bibliographic Details
Main Authors: Ping Gao, Xue Xiao, Zhuang Zhou, Hong Zhang, Raghupathi Subramanian, Anuchai Sinsawat, Xuelan Gu
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Biology
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Online Access:https://www.mdpi.com/2079-7737/14/3/266
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Summary:The epidermis acts as the body’s primary defense, relying on components like lipids, HA and GSH for skin barrier function, hydration and resistance to oxidative stress. However, limitations in the topical application of these biomolecules call for novel approaches. This study investigates the efficacy of Pro-GHL, a blend of free fatty acids, acetylglucosamine and GSH amino acid precursors (GAPs), designed to replenish skin lipids, HA and GSH through de novo biosynthesis. Using primary human keratinocytes, Pro-GHL demonstrated superior antioxidant and anti-inflammatory capacities compared to each individual component under the challenge of UVB or blue light. In 3D skin equivalent models (EpiKutis<sup>®</sup>), Pro-GHL enhanced skin barrier function. In addition, Pro-GHL prevented the development of pigmentation in pigmented 3D skin equivalent models (MelaKutis<sup>®</sup>) subjected to UVB irradiation or Benzo[a]pyrene exposure. Together, these results highlight Pro-GHL’s potential as a novel, effective and comprehensive skincare approach to fortify the skin’s defense system from within and prevent the accumulation of tissue damage in response to extrinsic stressors.
ISSN:2079-7737