Phylogeny of Flavobacteria Group Isolated from Freshwater Using Multilocus Sequencing Analysis
Sequence analysis of the 16S rRNA gene has been widely used for the classification of microorganisms. However, we have been unable to clearly identify five Flavobacterium species isolated from a freshwater by using the gene as a single marker, because the evolutionary history is incomplete and the p...
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BioMed Central
2013-12-01
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| Series: | Genomics & Informatics |
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| Online Access: | http://genominfo.org/upload/pdf/gni-11-272.pdf |
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| author | Seyoung Mun Jungnam Lee Siwon Lee Kyudong Han Tae-Young Ahn |
| author_facet | Seyoung Mun Jungnam Lee Siwon Lee Kyudong Han Tae-Young Ahn |
| author_sort | Seyoung Mun |
| collection | DOAJ |
| description | Sequence analysis of the 16S rRNA gene has been widely used for the classification of microorganisms. However, we have been unable to clearly identify five Flavobacterium species isolated from a freshwater by using the gene as a single marker, because the evolutionary history is incomplete and the pace of DNA substitutions is relatively rapid in the bacteria. In this study, we tried to classify Flavobacterium species through multilocus sequence analysis (MLSA), which is a practical and reliable technique for the identification or classification of bacteria. The five Flavobacterium species isolated from freshwater and 37 other strains were classified based on six housekeeping genes: gyrB, dnaK, tuf, murG, atpA, and glyA. The genes were amplified by PCR and subjected to DNA sequencing. Based on the combined DNA sequence (4,412 bp) of the six housekeeping genes, we analyzed the phylogenetic relationship among the Flavobacterium species. The results indicated that MLSA, based on the six housekeeping genes, is a trustworthy method for the identification of closely related Flavobacterium species. |
| format | Article |
| id | doaj-art-5e3c06788db74c078c8d6fa59da0db91 |
| institution | Kabale University |
| issn | 1598-866X 2234-0742 |
| language | English |
| publishDate | 2013-12-01 |
| publisher | BioMed Central |
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| series | Genomics & Informatics |
| spelling | doaj-art-5e3c06788db74c078c8d6fa59da0db912025-02-02T02:40:09ZengBioMed CentralGenomics & Informatics1598-866X2234-07422013-12-0111427227610.5808/GI.2013.11.4.27272Phylogeny of Flavobacteria Group Isolated from Freshwater Using Multilocus Sequencing AnalysisSeyoung Mun0Jungnam Lee1Siwon Lee2Kyudong Han3Tae-Young Ahn4Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan 330-714, Korea.Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan 330-714, Korea.Plant Quarantine Technology Center, Animal and Plant Quarantine Agency, Suwon 443-440, Korea.Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan 330-714, Korea.Department of Microbiology, Dankook University, Cheonan 330-714, Korea.Sequence analysis of the 16S rRNA gene has been widely used for the classification of microorganisms. However, we have been unable to clearly identify five Flavobacterium species isolated from a freshwater by using the gene as a single marker, because the evolutionary history is incomplete and the pace of DNA substitutions is relatively rapid in the bacteria. In this study, we tried to classify Flavobacterium species through multilocus sequence analysis (MLSA), which is a practical and reliable technique for the identification or classification of bacteria. The five Flavobacterium species isolated from freshwater and 37 other strains were classified based on six housekeeping genes: gyrB, dnaK, tuf, murG, atpA, and glyA. The genes were amplified by PCR and subjected to DNA sequencing. Based on the combined DNA sequence (4,412 bp) of the six housekeeping genes, we analyzed the phylogenetic relationship among the Flavobacterium species. The results indicated that MLSA, based on the six housekeeping genes, is a trustworthy method for the identification of closely related Flavobacterium species.http://genominfo.org/upload/pdf/gni-11-272.pdfmultilocus sequence typingphylogeny |
| spellingShingle | Seyoung Mun Jungnam Lee Siwon Lee Kyudong Han Tae-Young Ahn Phylogeny of Flavobacteria Group Isolated from Freshwater Using Multilocus Sequencing Analysis Genomics & Informatics multilocus sequence typing phylogeny |
| title | Phylogeny of Flavobacteria Group Isolated from Freshwater Using Multilocus Sequencing Analysis |
| title_full | Phylogeny of Flavobacteria Group Isolated from Freshwater Using Multilocus Sequencing Analysis |
| title_fullStr | Phylogeny of Flavobacteria Group Isolated from Freshwater Using Multilocus Sequencing Analysis |
| title_full_unstemmed | Phylogeny of Flavobacteria Group Isolated from Freshwater Using Multilocus Sequencing Analysis |
| title_short | Phylogeny of Flavobacteria Group Isolated from Freshwater Using Multilocus Sequencing Analysis |
| title_sort | phylogeny of flavobacteria group isolated from freshwater using multilocus sequencing analysis |
| topic | multilocus sequence typing phylogeny |
| url | http://genominfo.org/upload/pdf/gni-11-272.pdf |
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