A Lectin from Dioclea violacea Interacts with Midgut Surface of Lutzomyia migonei, Unlike Its Homologues, Cratylia floribunda Lectin and Canavalia gladiata Lectin
Leishmaniasis is a vector-borne disease transmitted by phlebotomine sand fly. Susceptibility and refractoriness to Leishmania depend on the outcome of multiple interactions that take place within the sand fly gut. Promastigote attachment to sand fly midgut epithelium is essential to avoid being excr...
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2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/239208 |
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| author | Juliana Montezuma Barbosa Monteiro Tínel Melina Fechine Costa Benevides Mércia Sindeaux Frutuoso Camila Farias Rocha Francisco Vassiliepe Sousa Arruda Mayron Alves Vasconcelos Francisco Nascimento Pereira-Junior João Batista Cajazeiras Kyria Santiago do Nascimento Jorge Luiz Martins Edson Holanda Teixeira Benildo Sousa Cavada Ricardo Pires dos Santos Margarida Maria Lima Pompeu |
| author_facet | Juliana Montezuma Barbosa Monteiro Tínel Melina Fechine Costa Benevides Mércia Sindeaux Frutuoso Camila Farias Rocha Francisco Vassiliepe Sousa Arruda Mayron Alves Vasconcelos Francisco Nascimento Pereira-Junior João Batista Cajazeiras Kyria Santiago do Nascimento Jorge Luiz Martins Edson Holanda Teixeira Benildo Sousa Cavada Ricardo Pires dos Santos Margarida Maria Lima Pompeu |
| author_sort | Juliana Montezuma Barbosa Monteiro Tínel |
| collection | DOAJ |
| description | Leishmaniasis is a vector-borne disease transmitted by phlebotomine sand fly. Susceptibility and refractoriness to Leishmania depend on the outcome of multiple interactions that take place within the sand fly gut. Promastigote attachment to sand fly midgut epithelium is essential to avoid being excreted together with the digested blood meal. Promastigote and gut sand fly surface glycans are important ligands in this attachment. The purpose of the present study was to evaluate the interaction of three lectins isolated from leguminous seeds (Diocleinae subtribe), D-glucose and D-mannose-binding, with glycans on Lutzomyia migonei midgut. To study this interaction the lectins were labeled with FITC and a fluorescence assay was performed. The results showed that only Dioclea violacea lectin (DVL) was able to interact with midgut glycans, unlike Cratylia floribunda lectin (CFL) and Canavalia gladiata lectin (CGL). Furthermore, when DVL was blocked with D-mannose the interaction was inhibited. Differences of spatial arrangement of residues and volume of carbohydrate recognition domain (CRD) may be the cause of the fine specificity of DVL for glycans in the surface on Lu. migonei midgut. The findings in this study showed the presence of glycans in the midgut with glucose/mannose residues in its composition and these residues may be important in interaction between Lu. migonei midgut and Leishmania. |
| format | Article |
| id | doaj-art-5e2ab94ac5b842c2918b09ef7681bf14 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-5e2ab94ac5b842c2918b09ef7681bf142025-02-03T05:43:49ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/239208239208A Lectin from Dioclea violacea Interacts with Midgut Surface of Lutzomyia migonei, Unlike Its Homologues, Cratylia floribunda Lectin and Canavalia gladiata LectinJuliana Montezuma Barbosa Monteiro Tínel0Melina Fechine Costa Benevides1Mércia Sindeaux Frutuoso2Camila Farias Rocha3Francisco Vassiliepe Sousa Arruda4Mayron Alves Vasconcelos5Francisco Nascimento Pereira-Junior6João Batista Cajazeiras7Kyria Santiago do Nascimento8Jorge Luiz Martins9Edson Holanda Teixeira10Benildo Sousa Cavada11Ricardo Pires dos Santos12Margarida Maria Lima Pompeu13Laboratory of Parasitology, Faculty of Medicine, Federal University of Ceará, 60430-160 Fortaleza, CE, BrazilLaboratory of Parasitology, Faculty of Medicine, Federal University of Ceará, 60430-160 Fortaleza, CE, BrazilLaboratory of Parasitology, Faculty of Medicine, Federal University of Ceará, 60430-160 Fortaleza, CE, BrazilLaboratory of Parasitology, Faculty of Medicine, Federal University of Ceará, 60430-160 Fortaleza, CE, BrazilIntegrated Laboratory of Biomolecules (LIBS), Department of Pathology and Legal Medicine, Faculty of Medicine, Federal University of Ceará, 60430-160 Fortaleza, CE, BrazilIntegrated Laboratory of Biomolecules (LIBS), Department of Pathology and Legal Medicine, Faculty of Medicine, Federal University of Ceará, 60430-160 Fortaleza, CE, BrazilLaboratory of Biologically Actives Molecules, Biochemistry and Molecular Biology Department, Federal University of Ceará, 60440-970 Fortaleza, CE, BrazilLaboratory of Biologically Actives Molecules, Biochemistry and Molecular Biology Department, Federal University of Ceará, 60440-970 Fortaleza, CE, BrazilLaboratory of Biologically Actives Molecules, Biochemistry and Molecular Biology Department, Federal University of Ceará, 60440-970 Fortaleza, CE, BrazilInstitute of Chemical and Geosciences, Federal University of Pelotas Institute of Chemical and Geosciences, Federal University of Pelotas, 96160-000 Pelotas, RS, BrazilIntegrated Laboratory of Biomolecules (LIBS), Department of Pathology and Legal Medicine, Faculty of Medicine, Federal University of Ceará, 60430-160 Fortaleza, CE, BrazilLaboratory of Biologically Actives Molecules, Biochemistry and Molecular Biology Department, Federal University of Ceará, 60440-970 Fortaleza, CE, BrazilComputer Engineering of Sobral, Federal University of Ceará, 62042-280 Sobral, CE, BrazilLaboratory of Parasitology, Faculty of Medicine, Federal University of Ceará, 60430-160 Fortaleza, CE, BrazilLeishmaniasis is a vector-borne disease transmitted by phlebotomine sand fly. Susceptibility and refractoriness to Leishmania depend on the outcome of multiple interactions that take place within the sand fly gut. Promastigote attachment to sand fly midgut epithelium is essential to avoid being excreted together with the digested blood meal. Promastigote and gut sand fly surface glycans are important ligands in this attachment. The purpose of the present study was to evaluate the interaction of three lectins isolated from leguminous seeds (Diocleinae subtribe), D-glucose and D-mannose-binding, with glycans on Lutzomyia migonei midgut. To study this interaction the lectins were labeled with FITC and a fluorescence assay was performed. The results showed that only Dioclea violacea lectin (DVL) was able to interact with midgut glycans, unlike Cratylia floribunda lectin (CFL) and Canavalia gladiata lectin (CGL). Furthermore, when DVL was blocked with D-mannose the interaction was inhibited. Differences of spatial arrangement of residues and volume of carbohydrate recognition domain (CRD) may be the cause of the fine specificity of DVL for glycans in the surface on Lu. migonei midgut. The findings in this study showed the presence of glycans in the midgut with glucose/mannose residues in its composition and these residues may be important in interaction between Lu. migonei midgut and Leishmania.http://dx.doi.org/10.1155/2014/239208 |
| spellingShingle | Juliana Montezuma Barbosa Monteiro Tínel Melina Fechine Costa Benevides Mércia Sindeaux Frutuoso Camila Farias Rocha Francisco Vassiliepe Sousa Arruda Mayron Alves Vasconcelos Francisco Nascimento Pereira-Junior João Batista Cajazeiras Kyria Santiago do Nascimento Jorge Luiz Martins Edson Holanda Teixeira Benildo Sousa Cavada Ricardo Pires dos Santos Margarida Maria Lima Pompeu A Lectin from Dioclea violacea Interacts with Midgut Surface of Lutzomyia migonei, Unlike Its Homologues, Cratylia floribunda Lectin and Canavalia gladiata Lectin The Scientific World Journal |
| title | A Lectin from Dioclea violacea Interacts with Midgut Surface of Lutzomyia migonei, Unlike Its Homologues, Cratylia floribunda Lectin and Canavalia gladiata Lectin |
| title_full | A Lectin from Dioclea violacea Interacts with Midgut Surface of Lutzomyia migonei, Unlike Its Homologues, Cratylia floribunda Lectin and Canavalia gladiata Lectin |
| title_fullStr | A Lectin from Dioclea violacea Interacts with Midgut Surface of Lutzomyia migonei, Unlike Its Homologues, Cratylia floribunda Lectin and Canavalia gladiata Lectin |
| title_full_unstemmed | A Lectin from Dioclea violacea Interacts with Midgut Surface of Lutzomyia migonei, Unlike Its Homologues, Cratylia floribunda Lectin and Canavalia gladiata Lectin |
| title_short | A Lectin from Dioclea violacea Interacts with Midgut Surface of Lutzomyia migonei, Unlike Its Homologues, Cratylia floribunda Lectin and Canavalia gladiata Lectin |
| title_sort | lectin from dioclea violacea interacts with midgut surface of lutzomyia migonei unlike its homologues cratylia floribunda lectin and canavalia gladiata lectin |
| url | http://dx.doi.org/10.1155/2014/239208 |
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