The Correlation between Gut Microbiota and Serum Metabolomic in Elderly Patients with Chronic Heart Failure
Objective. Chronic heart failure (CHF) refers to a state of persistent heart failure that can be stable, deteriorated, or decompensated. The mechanism and pathogenesis of myocardial remodeling remain unknown. Based on 16S rDNA sequencing and metabolomics technology, this study analyzed the gut micro...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2021/5587428 |
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| author | Zhenhua Wang Zhaoling Cai Markus W. Ferrari Yilong Liu Chengyi Li Tianzhang Zhang Guorong Lyu |
| author_facet | Zhenhua Wang Zhaoling Cai Markus W. Ferrari Yilong Liu Chengyi Li Tianzhang Zhang Guorong Lyu |
| author_sort | Zhenhua Wang |
| collection | DOAJ |
| description | Objective. Chronic heart failure (CHF) refers to a state of persistent heart failure that can be stable, deteriorated, or decompensated. The mechanism and pathogenesis of myocardial remodeling remain unknown. Based on 16S rDNA sequencing and metabolomics technology, this study analyzed the gut microbiota and serum metabolome in elderly patients with CHF to provide new insights into the microbiota and metabolic phenotypes of CHF. Methods. Blood and fecal samples were collected from 25 elderly patients with CHF and 25 healthy subjects. The expression of inflammatory factors in blood was detected by ELISA. 16S rDNA sequencing was used to analyze the changes in microorganisms in the samples. The changes of small molecular metabolites in serum samples were analyzed by LC-MS/MS. Spearman correlation coefficients were used to analyze the correlation between gut microbiota and serum metabolites. Results. Our results showed that the IL-6, IL-8, and TNF-α levels were significantly increased, and the IL-10 level was significantly decreased in the elderly patients with CHF compared with the healthy subjects. The diversity of the gut microbiota was decreased in the elderly patients with CHF. Moreover, Escherichia Shigella was negatively correlated with biocytin and RIBOFLAVIN. Haemophilus was negatively correlated with alpha-lactose, cellobiose, isomaltose, lactose, melibiose, sucrose, trehalose, and turanose. Klebsiella was positively correlated with bilirubin and ethylsalicylate. Klebsiella was negatively correlated with citramalate, hexanoylcarnitine, inosine, isovalerylcarnitine, methylmalonate, and riboflavin. Conclusion. The gut microbiota is simplified by the disease, and serum small-molecule metabolites evidently change in elderly patients with CHF. Serum and fecal biomarkers could be used for elderly patients with CHF screening. |
| format | Article |
| id | doaj-art-5deae955cc9e4e6c836a029d00449a19 |
| institution | Kabale University |
| issn | 1466-1861 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-5deae955cc9e4e6c836a029d00449a192025-02-03T06:06:31ZengWileyMediators of Inflammation1466-18612021-01-01202110.1155/2021/5587428The Correlation between Gut Microbiota and Serum Metabolomic in Elderly Patients with Chronic Heart FailureZhenhua Wang0Zhaoling Cai1Markus W. Ferrari2Yilong Liu3Chengyi Li4Tianzhang Zhang5Guorong Lyu6Department of CardiologyDepartment of CardiologyDepartment of Internal Medicine 1Department of CardiologyDepartment of CardiologyDepartment of CardiologyDepartment of Ultrasound MedicineObjective. Chronic heart failure (CHF) refers to a state of persistent heart failure that can be stable, deteriorated, or decompensated. The mechanism and pathogenesis of myocardial remodeling remain unknown. Based on 16S rDNA sequencing and metabolomics technology, this study analyzed the gut microbiota and serum metabolome in elderly patients with CHF to provide new insights into the microbiota and metabolic phenotypes of CHF. Methods. Blood and fecal samples were collected from 25 elderly patients with CHF and 25 healthy subjects. The expression of inflammatory factors in blood was detected by ELISA. 16S rDNA sequencing was used to analyze the changes in microorganisms in the samples. The changes of small molecular metabolites in serum samples were analyzed by LC-MS/MS. Spearman correlation coefficients were used to analyze the correlation between gut microbiota and serum metabolites. Results. Our results showed that the IL-6, IL-8, and TNF-α levels were significantly increased, and the IL-10 level was significantly decreased in the elderly patients with CHF compared with the healthy subjects. The diversity of the gut microbiota was decreased in the elderly patients with CHF. Moreover, Escherichia Shigella was negatively correlated with biocytin and RIBOFLAVIN. Haemophilus was negatively correlated with alpha-lactose, cellobiose, isomaltose, lactose, melibiose, sucrose, trehalose, and turanose. Klebsiella was positively correlated with bilirubin and ethylsalicylate. Klebsiella was negatively correlated with citramalate, hexanoylcarnitine, inosine, isovalerylcarnitine, methylmalonate, and riboflavin. Conclusion. The gut microbiota is simplified by the disease, and serum small-molecule metabolites evidently change in elderly patients with CHF. Serum and fecal biomarkers could be used for elderly patients with CHF screening.http://dx.doi.org/10.1155/2021/5587428 |
| spellingShingle | Zhenhua Wang Zhaoling Cai Markus W. Ferrari Yilong Liu Chengyi Li Tianzhang Zhang Guorong Lyu The Correlation between Gut Microbiota and Serum Metabolomic in Elderly Patients with Chronic Heart Failure Mediators of Inflammation |
| title | The Correlation between Gut Microbiota and Serum Metabolomic in Elderly Patients with Chronic Heart Failure |
| title_full | The Correlation between Gut Microbiota and Serum Metabolomic in Elderly Patients with Chronic Heart Failure |
| title_fullStr | The Correlation between Gut Microbiota and Serum Metabolomic in Elderly Patients with Chronic Heart Failure |
| title_full_unstemmed | The Correlation between Gut Microbiota and Serum Metabolomic in Elderly Patients with Chronic Heart Failure |
| title_short | The Correlation between Gut Microbiota and Serum Metabolomic in Elderly Patients with Chronic Heart Failure |
| title_sort | correlation between gut microbiota and serum metabolomic in elderly patients with chronic heart failure |
| url | http://dx.doi.org/10.1155/2021/5587428 |
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