Recent Advances in Three-Dimensional Stem Cell Culture Systems and Applications
Cell culture is one of the most core and fundamental techniques employed in the fields of biology and medicine. At present, although the two-dimensional cell culture method is commonly used in vitro, it is quite different from the cell growth microenvironment in vivo. In recent years, the limitation...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2021/9477332 |
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| author | Xiaowen Wu Junxiang Su Jizhen Wei Nan Jiang Xuejun Ge |
| author_facet | Xiaowen Wu Junxiang Su Jizhen Wei Nan Jiang Xuejun Ge |
| author_sort | Xiaowen Wu |
| collection | DOAJ |
| description | Cell culture is one of the most core and fundamental techniques employed in the fields of biology and medicine. At present, although the two-dimensional cell culture method is commonly used in vitro, it is quite different from the cell growth microenvironment in vivo. In recent years, the limitations of two-dimensional culture and the advantages of three-dimensional culture have increasingly attracted more and more attentions. Compared to two-dimensional culture, three-dimensional culture system is better to realistically simulate the local microenvironment of cells, promote the exchange of information among cells and the extracellular matrix (ECM), and retain the original biological characteristics of stem cells. In this review, we first present three-dimensional cell culture methods from two aspects: a scaffold-free culture system and a scaffold-based culture system. The culture method and cell characterizations will be summarized. Then the application of three-dimensional cell culture system is further explored, such as in the fields of drug screening, organoids and assembloids. Finally, the directions for future research of three-dimensional cell culture are stated briefly. |
| format | Article |
| id | doaj-art-5dc9101906b04f0da0ba8ce1c04e78a6 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-5dc9101906b04f0da0ba8ce1c04e78a62025-02-03T01:05:30ZengWileyStem Cells International1687-966X1687-96782021-01-01202110.1155/2021/94773329477332Recent Advances in Three-Dimensional Stem Cell Culture Systems and ApplicationsXiaowen Wu0Junxiang Su1Jizhen Wei2Nan Jiang3Xuejun Ge4Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Shanxi Medical University School and Hospital of Stomatology, Taiyuan 030001, ChinaShanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Shanxi Medical University School and Hospital of Stomatology, Taiyuan 030001, ChinaShanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Shanxi Medical University School and Hospital of Stomatology, Taiyuan 030001, ChinaCentral Laboratory, Peking University School and Hospital of Stomatology & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, ChinaShanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Shanxi Medical University School and Hospital of Stomatology, Taiyuan 030001, ChinaCell culture is one of the most core and fundamental techniques employed in the fields of biology and medicine. At present, although the two-dimensional cell culture method is commonly used in vitro, it is quite different from the cell growth microenvironment in vivo. In recent years, the limitations of two-dimensional culture and the advantages of three-dimensional culture have increasingly attracted more and more attentions. Compared to two-dimensional culture, three-dimensional culture system is better to realistically simulate the local microenvironment of cells, promote the exchange of information among cells and the extracellular matrix (ECM), and retain the original biological characteristics of stem cells. In this review, we first present three-dimensional cell culture methods from two aspects: a scaffold-free culture system and a scaffold-based culture system. The culture method and cell characterizations will be summarized. Then the application of three-dimensional cell culture system is further explored, such as in the fields of drug screening, organoids and assembloids. Finally, the directions for future research of three-dimensional cell culture are stated briefly.http://dx.doi.org/10.1155/2021/9477332 |
| spellingShingle | Xiaowen Wu Junxiang Su Jizhen Wei Nan Jiang Xuejun Ge Recent Advances in Three-Dimensional Stem Cell Culture Systems and Applications Stem Cells International |
| title | Recent Advances in Three-Dimensional Stem Cell Culture Systems and Applications |
| title_full | Recent Advances in Three-Dimensional Stem Cell Culture Systems and Applications |
| title_fullStr | Recent Advances in Three-Dimensional Stem Cell Culture Systems and Applications |
| title_full_unstemmed | Recent Advances in Three-Dimensional Stem Cell Culture Systems and Applications |
| title_short | Recent Advances in Three-Dimensional Stem Cell Culture Systems and Applications |
| title_sort | recent advances in three dimensional stem cell culture systems and applications |
| url | http://dx.doi.org/10.1155/2021/9477332 |
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