Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901
Embryonic stem cells (ESCs) have the ability to grow indefinitely and retain their pluripotency in culture, and this self-renewal capacity is governed by several crucial molecular pathways controlled by specific regulatory genes and epigenetic modifications. It is reported that multiple epigenetic r...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/1792573 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832563775921717248 |
|---|---|
| author | Zhiying Ai Jingjing Shao Xinglong Shi Mengying Yu Yongyan Wu Juan Du Yong Zhang Zekun Guo |
| author_facet | Zhiying Ai Jingjing Shao Xinglong Shi Mengying Yu Yongyan Wu Juan Du Yong Zhang Zekun Guo |
| author_sort | Zhiying Ai |
| collection | DOAJ |
| description | Embryonic stem cells (ESCs) have the ability to grow indefinitely and retain their pluripotency in culture, and this self-renewal capacity is governed by several crucial molecular pathways controlled by specific regulatory genes and epigenetic modifications. It is reported that multiple epigenetic regulators, such as miRNA and pluripotency factors, can be tightly integrated into molecular pathways and cooperate to maintain self-renewal of ESCs. However, mouse ESCs in serum-containing medium seem to be heterogeneous due to the self-activating differentiation signal of MEK/ERK. Thus, to seek for the crucial miRNA and key regulatory genes that establish ESC properties in MEK/ERK pathway, we performed microarray analysis and small RNA deep-sequencing of J1 mESCs treated with or without PD0325901 (PD), a well-known inhibitor of MEK/ERK signal pathway, followed by verification of western blot analysis and quantitative real-time PCR verification; we found that PD regulated the transcript expressions related to self-renewal and differentiation and antagonized the action of retinoic acid- (RA-) induced differentiation. Moreover, PD can significantly modulate the expressions of multiple miRNAs that have crucial functions in ESC development. Overall, our results demonstrate that PD could enhance ESC self-renewal capacity both by key regulatory genes and ES cell-specific miRNA, which in turn influences ESC self-renewal and cellular differentiation. |
| format | Article |
| id | doaj-art-5d9a7e96f741466db53845462cf30a3a |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-5d9a7e96f741466db53845462cf30a3a2025-02-03T01:12:34ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/17925731792573Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901Zhiying Ai0Jingjing Shao1Xinglong Shi2Mengying Yu3Yongyan Wu4Juan Du5Yong Zhang6Zekun Guo7College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, ChinaCollege of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, ChinaCollege of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, ChinaKey Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, ChinaKey Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, ChinaCollege of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, ChinaKey Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, ChinaKey Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling, Shaanxi 712100, ChinaEmbryonic stem cells (ESCs) have the ability to grow indefinitely and retain their pluripotency in culture, and this self-renewal capacity is governed by several crucial molecular pathways controlled by specific regulatory genes and epigenetic modifications. It is reported that multiple epigenetic regulators, such as miRNA and pluripotency factors, can be tightly integrated into molecular pathways and cooperate to maintain self-renewal of ESCs. However, mouse ESCs in serum-containing medium seem to be heterogeneous due to the self-activating differentiation signal of MEK/ERK. Thus, to seek for the crucial miRNA and key regulatory genes that establish ESC properties in MEK/ERK pathway, we performed microarray analysis and small RNA deep-sequencing of J1 mESCs treated with or without PD0325901 (PD), a well-known inhibitor of MEK/ERK signal pathway, followed by verification of western blot analysis and quantitative real-time PCR verification; we found that PD regulated the transcript expressions related to self-renewal and differentiation and antagonized the action of retinoic acid- (RA-) induced differentiation. Moreover, PD can significantly modulate the expressions of multiple miRNAs that have crucial functions in ESC development. Overall, our results demonstrate that PD could enhance ESC self-renewal capacity both by key regulatory genes and ES cell-specific miRNA, which in turn influences ESC self-renewal and cellular differentiation.http://dx.doi.org/10.1155/2016/1792573 |
| spellingShingle | Zhiying Ai Jingjing Shao Xinglong Shi Mengying Yu Yongyan Wu Juan Du Yong Zhang Zekun Guo Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901 Stem Cells International |
| title | Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901 |
| title_full | Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901 |
| title_fullStr | Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901 |
| title_full_unstemmed | Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901 |
| title_short | Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901 |
| title_sort | maintenance of self renewal and pluripotency in j1 mouse embryonic stem cells through regulating transcription factor and microrna expression induced by pd0325901 |
| url | http://dx.doi.org/10.1155/2016/1792573 |
| work_keys_str_mv | AT zhiyingai maintenanceofselfrenewalandpluripotencyinj1mouseembryonicstemcellsthroughregulatingtranscriptionfactorandmicrornaexpressioninducedbypd0325901 AT jingjingshao maintenanceofselfrenewalandpluripotencyinj1mouseembryonicstemcellsthroughregulatingtranscriptionfactorandmicrornaexpressioninducedbypd0325901 AT xinglongshi maintenanceofselfrenewalandpluripotencyinj1mouseembryonicstemcellsthroughregulatingtranscriptionfactorandmicrornaexpressioninducedbypd0325901 AT mengyingyu maintenanceofselfrenewalandpluripotencyinj1mouseembryonicstemcellsthroughregulatingtranscriptionfactorandmicrornaexpressioninducedbypd0325901 AT yongyanwu maintenanceofselfrenewalandpluripotencyinj1mouseembryonicstemcellsthroughregulatingtranscriptionfactorandmicrornaexpressioninducedbypd0325901 AT juandu maintenanceofselfrenewalandpluripotencyinj1mouseembryonicstemcellsthroughregulatingtranscriptionfactorandmicrornaexpressioninducedbypd0325901 AT yongzhang maintenanceofselfrenewalandpluripotencyinj1mouseembryonicstemcellsthroughregulatingtranscriptionfactorandmicrornaexpressioninducedbypd0325901 AT zekunguo maintenanceofselfrenewalandpluripotencyinj1mouseembryonicstemcellsthroughregulatingtranscriptionfactorandmicrornaexpressioninducedbypd0325901 |